Phosphoproteomic studies in Arabidopsis and tobacco male gametophytes

2014 ◽  
Vol 42 (2) ◽  
pp. 383-387 ◽  
Author(s):  
Jan Fíla ◽  
Věra Čapková ◽  
David Honys
Keyword(s):  
Amino Acids ◽  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Nicotiana Tabacum ◽  
Pollen Tube ◽  
Protein Phosphorylation ◽  
Pollen Tube Growth ◽  
Mature Pollen ◽  
Phosphorylation Sites ◽  
Post Translational Modifications

Mature pollen represents an extremely resistant quiescent structure surrounded by a tough cell wall. After its hydration on stigma papillary cells, pollen tube growth starts rapidly. Massive metabolic changes are likely to be accompanied by changes in protein phosphorylation. Protein phosphorylation belongs among the most rapid post-translational modifications. To date, only Arabidopsis thaliana and tobacco (Nicotiana tabacum) mature pollen have been subjected to phosphoproteomic studies in order to identify the phosphoproteins present. In the present mini-review, Arabidopsis and tobacco datasets were compared with each other. The representation of the O-phosphorylated amino acids was compared between these two datasets, and the putative pollen-specific or pollen-abundant phosphopeptides were highlighted. Finally, the phosphorylation sites common for both Arabidopsis and tobacco phosphoproteins are listed as well as the phosphorylation motifs identified.

BcSKS11, a SKS gene from pollinated pistils of Chinese cabbage pak choi, is expressed continuously during the male gametophyte development, pollen germination and pollen-tube growth processes

2013 ◽  
Vol 61 (6) ◽  
pp. 446
Author(s):  
Zhixian Zhang ◽  
Jiashu Cao ◽  
Lin Qiu ◽  
Jingjing Jiang ◽  
Baohua Wu
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Chinese Cabbage ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
In Situ Hybridisation ◽  
Pollen Grains ◽  
Tube Growth ◽  
Mature Pollen ◽  
Pak Choi

Cell wall-related genes and proteins are crucial for fertilisation-related events such as pollen germination and pollen-tube growth. Recent studies have focussed on the molecular mechanism of cell-wall synthesis and regulation of these processes; however, limited information is available on the mechanism and modification of the crucial cell-wall materials. We isolated a gene that is potentially involved in cell-wall metabolism from pollinated pistils of the male sterile line ‘Bcajh97-A’ in Chinese cabbage pak choi (Brassica campestris SKewed5 Similar 11, BcSKS11). BcSKS11 has several features of SKewed5 Similar (SKS) proteins, which are structures that are similar to plant multicopper oxidase but lack the 10 necessary residues of two copper-binding sites. A phylogenetic tree constructed from 49 SKS proteins found four clades, with BcSKS11 being in a clade with tobacco sequences. Reverse transcription–polymerase chain reaction and in situ hybridisation revealed that BcSKS11 was specifically expressed in uninucleate microspores, mature pollen grains and growing pollen tubes of pollinated pistils. These results suggest that BcSKS11 might be stored in mature pollen grains, in preparation for pollen germination and pollen-tube growth.

scholarly journals Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 249
Author(s):  
Weimiao Liu ◽  
Liai Xu ◽  
Hui Lin ◽  
Jiashu Cao
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Cell Walls ◽  
Root Elongation ◽  
Expression Patterns ◽  
Primary Root ◽  
Pollen Grains ◽  
Tube Growth ◽  
Cell Wall Binding

The growth of plant cells is inseparable from relaxation and expansion of cell walls. Expansins are a class of cell wall binding proteins, which play important roles in the relaxation of cell walls. Although there are many members in expansin gene family, the functions of most expansin genes in plant growth and development are still poorly understood. In this study, the functions of two expansin genes, AtEXPA4 and AtEXPB5 were characterized in Arabidopsis thaliana. AtEXPA4 and AtEXPB5 displayed consistent expression patterns in mature pollen grains and pollen tubes, but AtEXPA4 also showed a high expression level in primary roots. Two single mutants, atexpa4 and atexpb5, showed normal reproductive development, whereas atexpa4atexpb5 double mutant was defective in pollen tube growth. Moreover, AtEXPA4 overexpression enhanced primary root elongation, on the contrary, knocking out AtEXPA4 made the growth of primary root slower. Our results indicated that AtEXPA4 and AtEXPB5 were redundantly involved in pollen tube growth and AtEXPA4 was required for primary root elongation.

scholarly journals Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube

Plants ◽  
2013 ◽  
Vol 2 (3) ◽  
pp. 429-440 ◽  
Author(s):  
Mário da Costa ◽  
Luís Pereira ◽  
Sílvia Coimbra
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Pollen Tube ◽  
Growth Media

scholarly journals Transcriptome and translatome changes in germinated pollen under heat stress uncover roles of transporter genes involved in pollen tube growth

2020 ◽  
Author(s):  
Laetitia Poidevin ◽  
Javier Forment ◽  
Dilek Unal ◽  
Alejandro Ferrando
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Heat Shock Genes ◽  
Germinated Pollen

ABSTRACTPlant reproduction is one key biological process very sensitive to heat stress and, as a consequence, enhanced global warming poses serious threats to food security worldwide. In this work we have used a high-resolution ribosome profiling technology to study how heat affects both the transcriptome and the translatome of Arabidopsis thaliana pollen germinated in vitro. Overall, a high correlation between transcriptional and translational responses to high temperature was found, but specific regulations at the translational level were also present. We show that bona fide heat shock genes are induced by high temperature indicating that in vitro germinated pollen is a suitable system to understand the molecular basis of heat responses. Concurrently heat induced significant down-regulation of key membrane transporters required for pollen tube growth, thus uncovering heat-sensitive targets. We also found that a large subset of the heat-repressed transporters is specifically up-regulated, in a coordinated manner, with canonical heat-shock genes in pollen tubes grown in vitro and semi in vivo, based on published transcriptomes from Arabidopsis thaliana. Ribosome footprints were also detected in gene sequences annotated as non-coding, highlighting the potential for novel translatable genes and translational dynamics.

scholarly journals Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O‐arabinosyltransferase mutants

2020 ◽  
Vol 103 (4) ◽  
pp. 1399-1419
Author(s):  
Steven Beuder ◽  
Alexandria Dorchak ◽  
Ashwini Bhide ◽  
Svenning Rune Moeller ◽  
Bent L. Petersen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Structural Defects

scholarly journals A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Ferdousse Laggoun ◽  
Flavien Dardelle ◽  
Jérémy Dehors ◽  
Denis Falconet ◽  
Azeddine Driouich ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Chemical Screen

scholarly journals To preserve or to destroy, that is the question: the role of the cell wall integrity pathway in pollen tube growth

2019 ◽  
Vol 52 ◽  
pp. 131-139 ◽  
Author(s):  
Hannes Vogler ◽  
Gorka Santos-Fernandez ◽  
Martin A Mecchia ◽  
Ueli Grossniklaus
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Cell Wall Integrity ◽  
Tube Growth ◽  
Cell Wall Integrity Pathway

Ethylene response to pollen tube growth in Nicotiana tabacum flowers

Planta ◽  
2002 ◽  
Vol 214 (5) ◽  
pp. 806-812 ◽  
Author(s):  
D. De Martinis ◽  
Cotti G. ◽  
S. te Lintel Hekker ◽  
Harren F. ◽  
Mariani C.
Keyword(s):  
Nicotiana Tabacum ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Ethylene Response

scholarly journals Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e55411 ◽  
Author(s):  
Li Wang ◽  
Xueqin Lv ◽  
Hong Li ◽  
Min Zhang ◽  
Hong Wang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Calcium Homeostasis ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Cedrus Deodara
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