The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei

2005 ◽  
Vol 33 (5) ◽  
pp. 983 ◽  
Author(s):  
T.K. Smith ◽  
K.L. Martin
Keyword(s):  
Trypanosoma Brucei ◽  
Inositol 1 ◽  
Phosphate Synthase

The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei

2005 ◽  
Vol 33 (5) ◽  
pp. 983-985 ◽  
Author(s):  
K.L. Martin ◽  
T.K. Smith
Keyword(s):  
Trypanosoma Brucei ◽  
De Novo ◽  
Essential Gene ◽  
Recombinant Expression ◽  
Conditional Knockout ◽  
Cellular Functions ◽  
Genome Database ◽  
Inositol 1 ◽  
Catalytically Active ◽  
Phosphate Synthase

The de novo synthesis of myo-inositol occurs via a two-step process: first, glucose 6-phosphate is converted into inositol 1-phosphate by an INO1 (myo-inositol-1-phosphate synthase; EC 5.5.1.4); then, it is dephosphorylated by an inositol monophosphatase. The myo-inositol can then be incorporated into PI (phosphatidylinositol), which is utilized in a variety of cellular functions, including the biosynthesis of GPI (glycosylphosphatidylinositol) anchors. A putative INO1 was identified in the Trypanosoma brucei genome database and, by recombinant expression in Escherichia coli, was shown to be a catalytically active INO1. To investigate the importance of INO1, we created a conditional knockout, which, under non-permissive conditions, showed that INO1 is an essential gene in bloodstream form T. brucei and that the de novo synthesized myo-inositol is used for the formation of PI and GPI anchors.

scholarly journals A Novel Salt-tolerant l-myo-Inositol-1-phosphate Synthase fromPorteresia coarctata(Roxb.) Tateoka, a Halophytic Wild Rice

2004 ◽  
Vol 279 (27) ◽  
pp. 28539-28552 ◽  
Author(s):  
Manoj Majee ◽  
Susmita Maitra ◽  
Krishnarup Ghosh Dastidar ◽  
Sitakanta Pattnaik ◽  
Anirban Chatterjee ◽  
...  
Keyword(s):  
Wild Rice ◽  
Salt Tolerant ◽  
Inositol 1 ◽  
Phosphate Synthase

Myo-inositol-1-phosphate synthase from streptomyces griseus (Studies on the biosynthesis of cyclitols, XXXVIII1)

1979 ◽  
Vol 25 (1) ◽  
Author(s):  
Fritz Pittner ◽  
IrinaI. Tovarova ◽  
ElenaYa. Kornitskaya ◽  
AleksanderS. Khokhlov ◽  
Otto Hoffmann-Ostenhof
Keyword(s):  
Streptomyces Griseus ◽  
Inositol 1 ◽  
Phosphate Synthase

scholarly journals Chloroplast as a Locale of L-myo-Inositol-1-Phosphate Synthase

1987 ◽  
Vol 85 (3) ◽  
pp. 611-614 ◽  
Author(s):  
Jukta Adhikari ◽  
Asima Lahiri Majumder ◽  
Tirtha Jyoti Bhaduri ◽  
Sarmila DasGupta ◽  
Arun Lahiri Majumder
Keyword(s):  
Inositol 1 ◽  
Phosphate Synthase
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