scholarly journals The ‘super-resolution’ revolution

2009 ◽  
Vol 37 (5) ◽  
pp. 1042-1044 ◽  
Author(s):  
Ilan Davis
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Light Sheet ◽  
Live Cell ◽  
Stimulated Emission ◽  
Structured Illumination ◽  
Light Sheet Microscopy ◽  
Resolution Imaging ◽  
New Instruments

We are currently in the midst of an exciting revolution in microscopy. In many ways, this has been happening for several decades, but it is the rate of development of new methods that has increased recently. The last few years have seen an impressive proliferation of new instruments for imaging at higher resolution, imaging single molecules and faster and more sensitive multidimensional live cell imaging. These include light sheet microscopy, stimulated emission depletion, structured illumination and live cell imaging on the OMX (optical microscopy experimental) platform. However, new probes and image analysis methods have also been crucial for the development of these revolutionary methods.

scholarly journals Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging

Biosensors ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 330
Author(s):  
Jia Zhang ◽  
Xinwei Gao ◽  
Luwei Wang ◽  
Yong Guo ◽  
Yinru Zhu ◽  
...  
Keyword(s):  
Low Power ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Stimulated Emission ◽  
Live Cells ◽  
Sted Microscopy ◽  
Resolution Imaging ◽  
Stimulated Emission Depletion

Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria and microtubules in live cells, respectively, with 0.8 mW depletion power. These results highlight the great potential of the novel digitally enhanced two-color STED microscopy for long-term dynamic imaging of live cells.

Live Cell Super-Resolution Imaging with N-SIM

2012 ◽  
Vol 20 (4) ◽  
pp. 18-21
Author(s):  
Christopher B. O'Connell
Keyword(s):  
Light Microscope ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Specific Proteins ◽  
Resolution Imaging ◽  
Patterned Illumination

The ability to visualize the distributions of specific proteins with a light microscope and fluorescent probes is largely responsible for our current understanding of cellular structure. A major limitation of this approach arises from the blurring effects of diffraction, which decreases resolution and limits the ability to obtain information at the nanoscale. There has been a tremendous drive to develop optical and computational methods that improve the resolution of the light microscope, and structured illumination microscopy (SIM) is one solution. This method uses patterned illumination to double both lateral and axial resolution. Nikon's N-SIM is a commercial system that integrates the most desirable features of light microscopy, specific labeling of molecules, and live cell imaging, with structured illumination. This provides the ability to achieve super resolution suitable for a range of biological applications.

A millisecond structured illumination microscope for super-resolution live cell imaging

2020 ◽  
Vol 13 (4) ◽  
pp. 045002
Author(s):  
Tomu Suzuki ◽  
Shinji Kajimoto ◽  
Narufumi Kitamura ◽  
Mayumi Takano-Kasuya ◽  
Naoko Furusawa ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Structured Illumination

scholarly journals Advances in High-Speed Structured Illumination Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Tianyu Zhao ◽  
Zhaojun Wang ◽  
Tongsheng Chen ◽  
Ming Lei ◽  
Baoli Yao ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Power ◽  
Recent Developments ◽  
Super Resolution Microscopy

Super-resolution microscopy surpasses the diffraction limit to enable the observation of the fine details in sub-cellular structures and their dynamics in diverse biological processes within living cells. Structured illumination microscopy (SIM) uses a relatively low illumination light power compared with other super-resolution microscopies and has great potential to meet the demands of live-cell imaging. However, the imaging acquisition and reconstruction speeds limit its further applications. In this article, recent developments all targeted at improving the overall speed of SIM are reviewed. These comprise both hardware and software improvements, which include a reduction in the number of raw images, GPU acceleration, deep learning and the spatial domain reconstruction. We also discuss the application of these developments in live-cell imaging.

Advances in Microscopy Techniques

2011 ◽  
Vol 135 (2) ◽  
pp. 255-263
Author(s):  
Daniel B Schmolze ◽  
Clive Standley ◽  
Kevin E Fogarty ◽  
Andrew H Fischer
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Near Field ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Molecular Techniques ◽  
Live Cell ◽  
Stimulated Emission ◽  
Structured Illumination ◽  
Microscopy Techniques

Abstract Context.—Advances in microscopy enable visualization of a broad range of new morphologic features. Objective.—To review and illustrate advances in microscopy with relevance to pathologists. Data Sources.—Literature review and new observations. Results.—Fluorescence microscopy enables multiantigen detection; allows novel optical-sectioning techniques, with some advantages compared to paraffin sectioning; and permits live-cell imaging. Live-cell imaging allows pathologists to move from a period when all diagnostic expertise was reliant on interpreting static images to a period when cellular dynamics can play a role in diagnosis. New techniques have bypassed by about 100-fold what had long been believed to be a limit to the resolution of light microscopy. Fluorescence resonance energy transfer (FRET) appears capable of visualizing diagnostically relevant molecular events in living or fixed cells that are immeasurable by other molecular techniques. We describe applications of 2-photon microscopy, FRET, structured illumination, and the subdiffraction techniques of near-field microscopy, photoactivated localization microscopy, stochastic optical reconstruction microscopy, and stimulated emission depletion microscopy. Conclusion.—New microscopy techniques present opportunities for pathologists to develop improved diagnostic tests.

scholarly journals Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging

2017 ◽  
Vol 67 (2) ◽  
pp. 282-293.e7 ◽  
Author(s):  
Tadasu Nozaki ◽  
Ryosuke Imai ◽  
Mai Tanbo ◽  
Ryosuke Nagashima ◽  
Sachiko Tamura ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Chromatin Domains ◽  
Dynamic Organization

scholarly journals A Novel Digital-Micromirror Device Based Structured Illumination Microscope for Multicolor Live Cell Imaging

2021 ◽  
Vol 120 (3) ◽  
pp. 179a
Author(s):  
Peter T. Brown ◽  
Rory Kruithoff ◽  
Gregory J. Seedorf ◽  
Douglas P. Shepherd
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Digital Micromirror Device ◽  
Structured Illumination
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