Moving proteins from the cytosol into mitochondria

2004 ◽  
Vol 32 (5) ◽  
pp. 774-776 ◽  
Author(s):  
A. Chacinska ◽  
P. Rehling
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Function ◽  
Mitochondrial Membranes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Great Complexity ◽  
Cytosolic Ribosomes

Mitochondria of the yeast Saccharomyces cerevisiae contain at least 750 different proteins, which perform diverse roles. Most of these proteins (approx. 99%) are translated on cytosolic ribosomes, and their import into mitochondria is essential for mitochondrial function. Proteinaceous machineries of great complexity, the so-called translocases, in the mitochondrial membranes mediate the import of these proteins.

scholarly journals Anhydrobiosis in Yeasts: Changes in Mitochondrial Membranes Improve the Resistance of Saccharomyces cerevisiae Cells to Dehydration–Rehydration

Fermentation ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 82 ◽  
Author(s):  
Galina Khroustalyova ◽  
Alexander Rapoport
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Cell ◽  
Cell Survival ◽  
Yeast Species ◽  
Lithocholic Acid ◽  
Yeast Cells ◽  
Mitochondrial Membranes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Modern Biotechnology ◽  
Main Factors

Anhydrobiosis is a unique state of live organisms in which their metabolism is temporary reversibly suspended as the result of strong dehydration of their cells. This state is widely used currently during large-capacity production of active dry baker’s yeast. Other strains of the yeast Saccharomyces cerevisiae, as well as other yeast species that could potentially find use in modern biotechnology, are not resistant to dehydration–rehydration treatments. To improve their resistance, the main factors that influence cell survival during such treatment need to be revealed. This study showed the importance of mitochondria for yeast cell survival during transfer into anhydrobiosis, a factor that was strongly underestimated until this study. It was revealed that the external introduction inside yeast cells of 50 μM of lithocholic acid (LCA), an agent that induces changes in glycerophospholipids in mitochondrial membranes, in combination with 1% DMSO, may improve the survival rate of dehydrated cells. The influence of LCA upon yeast cell resistance to dehydration–rehydration was not linked with changes in the state of the cells’ plasma membrane.

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