The life and work of Dermot Hedley (‘Derek’) Williamson (1929–1998)

2001 ◽  
Vol 29 (2) ◽  
pp. 237-240
Author(s):  
R. D. Evans ◽  
M. Stubbs ◽  
G. F. Gibbons ◽  
E. A. Newsholme
Keyword(s):  
Metabolic Pathways ◽  
Metabolic Regulation ◽  
Ketone Body ◽  
Scientific Discovery ◽  
Lipid Synthesis ◽  
Acid Oxidation ◽  
Citation Classic ◽  
Integrated Regulation ◽  
Ketone Body Metabolism

Derek Williamson's scientific career spanned the ‘Golden Age’ of research into metabolic regulation, to which he made an important and sustained contribution. Derek joined Hans Krebs' laboratory at Sheffield University in 1946 and moved to Krebs' MRC Unit in Oxford in 1960. He elaborated an enzymic method for the determination of acetoacetate and 3-hydroxybutyrate [Williamson, Mellanby and Krebs, Biochem. J. (1962) 82, 90–96], which opened up the field of ketone body metabolism and its regulation and became a Citation Classic. Another Citation Classic followed [Williamson, Lund and Krebs, Biochem. J. (1967) 103, 514–527]. He moved with Krebs to the Metabolic Research Laboratory at the Radcliffe Infirmary in 1967, where he blossomed, formulating his ideas about the integrated regulation of metabolic pathways, particularly with regard to fatty acid oxidation, lipid synthesis and ketone body metabolism. His success was illustrated by more than 200 publications. Derek implanted and nurtured a sense of the excitement of scientific discovery in his colleagues and students, and he worked hard to provide a friendly, supportive and encouraging environment. Many lives have been enriched by the privilege of working with him.

KETONE BODY METABOLISM IN NUTRITIONAL MYOPATHY

1964 ◽  
Vol 42 (8) ◽  
pp. 1153-1160 ◽  
Author(s):  
K. J. Jenkins
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Ketone Body ◽  
Ketone Bodies ◽  
Pectoral Muscle ◽  
Acid Oxidation ◽  
Dystrophic Muscle ◽  
Liver Homogenates ◽  
Ketone Body Metabolism

A study was conducted on the metabolism of ketone bodies in tissue preparations from normal and dystrophic chicks. The data indicated that the production of ketone bodies in liver homogenates, as a result of fatty acid oxidation, was not markedly altered by development of the dystrophic condition. Whereas acetoacetate was oxidized by normal and degenerative pectoral muscle to approximately the same extent, utilization of β-hydroxybutyrate in dystrophic muscle was markedly poorer. In view of present concepts of the reactions involved in the metabolism of ketone bodies the results suggest that in the chick myopathy the conversion of β-hydroxybutyrate to acetoacetate may be impaired.

Ketone body metabolism in NIDDM. Effect of sulfonylurea treatment

Diabetes ◽  
1992 ◽  
Vol 41 (8) ◽  
pp. 968-974 ◽  
Author(s):  
A. Avogaro ◽  
A. Valerio ◽  
L. Gnudi ◽  
A. Maran ◽  
M. Zolli ◽  
...  
Keyword(s):  
Ketone Body ◽  
Ketone Body Metabolism

Authenticity of sugar – Identification of plant source and determination of geographical origin of sucrose

2015 ◽  
pp. 40-43 ◽  
Author(s):  
Andreas G. Degenhardt
Keyword(s):  
Metabolic Pathways ◽  
Isotope Ratio ◽  
Geographical Origin ◽  
Hydrological Cycle ◽  
Isotope Ratios ◽  
Saccharum Officinarum ◽  
Mass Spectrometric ◽  
C3 And C4 Plants ◽  
Plant Source

The isotope ratios of water, organic matter and micronutrients from food are dependent on the circumstances and sites of their origin and production. Analytical methods, based on mass spectrometry, are established for routine determination of isotopes. Differentiation between metabolic pathways of C3 and C4 plants is realizable by determination 13C/12C ratios which can distinguish and identify sucrose from pure beet (Beta vulgaris) and pure cane (Saccharum officinarum). Influenced by the worldwide hydrological cycle the isotope ratios of 2H/1H and 18O/16O vary systematically, the variations give information about geographical origin. The exemplarily determination of authenticity is demonstrated by using mass spectrometric isotope ratio evaluation for identification of plant source and geographical origin with the help of selected sugar samples with known origin.

scholarly journals Enzymes of Ketone Body Metabolism

1960 ◽  
Vol 235 (2) ◽  
pp. 318-325 ◽  
Author(s):  
George I. Drummond ◽  
Joseph R. Stern
Keyword(s):  
Ketone Body ◽  
Ketone Body Metabolism

scholarly journals Variations in Triterpenoid Deposition in Cuticular Waxes during Development and Maturation of Selected Fruits of Rosaceae Family

2020 ◽  
Vol 21 (24) ◽  
pp. 9762
Author(s):  
Soyol Dashbaldan ◽  
Cezary Pączkowski ◽  
Anna Szakiel
Keyword(s):  
Metabolic Pathways ◽  
Cuticular Waxes ◽  
Rosa Rugosa ◽  
Phenological Stages ◽  
Aronia Melanocarpa ◽  
Fruit Samples ◽  
Black Chokeberry ◽  
Rosaceae Family ◽  
Fruit Surface

The process of fruit ripening involves many chemical changes occurring not only in the mesocarp but also in the epicarp, including changes in the triterpenoid content of fruit cuticular waxes that can modify the susceptibility to pathogens and mechanical properties of the fruit surface. The aim of the study was the determination of the ripening-related changes in the triterpenoid content of fruit cuticular waxes of three plant species from the Rosaceae family, including rugosa rose (Rosa rugosa), black chokeberry (Aronia melanocarpa var. “Galicjanka”) and apple (Malus domestica var. “Antonovka”). The triterpenoid and steroid content in chloroform-soluble cuticular waxes was determined by a GC-MS/FID method at four different phenological stages. The profile of identified compounds was rather similar in selected fruit samples with triterpenoids with ursane-, oleanane- and lupane-type carbon skeletons, prevalence of ursolic acid and the composition of steroids. Increasing accumulation of triterpenoids and steroids, as well as the progressive enrichment of the composition of these compounds in cuticular wax during fruit development, was observed. The changes in triterpenoid content resulted from modifications of metabolic pathways, particularly hydroxylation and esterification, that can alter interactions with complementary functional groups of aliphatic constituents and lead to important changes in fruit surface quality.

Colorimetric Determination of Hydroxyproline as Measure of Collagen Content in Meat and Meat Products: NMKL Collaborative Study

1990 ◽  
Vol 73 (1) ◽  
pp. 54-57 ◽  
Author(s):  
Kurt Kolar
Keyword(s):  
Collaborative Study ◽  
Colorimetric Method ◽  
Meat Products ◽  
Acid Oxidation ◽  
Colorimetric Determination ◽  
Mean Values ◽  
Collaborative Studies ◽  
Freeze Dried ◽  
Analytical Result

Abstract A colorimetric method for the determination of hydroxyproline as a measure of collagen in meat and meat products has been collaboratively studied in 18 laboratories. The method includes hydrolysis with sulfuric acid, oxidation with chloramine- T, and formation of a reddish purple complex with 4- dimethylaminobenzaldehyde. Five frozen and 3 freeze-dried samples were tested, ranging in content from 0.11 to 0.88% and from 0.39 to 4.0% hydroxyproline, respectively. The mean values of 2 identical samples were 0.245 and 0.251 %. The average recovery from a spiked sample was 96.1 %. The hydroxyproline content of a known sample (a mixture of 2 samples in the ratio 5:2) was calculated to 1.42%, which agrees well with the analytical result, 1.40%. In comparison with other collaborative studies, based on the ISO analytical method, the repeatability and reproducibility of this method agree well with the other results. This method was accepted as an official NMKL method by all national Committees, and has been adopted official first action by AOAC as an NMKLAOAC method.

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