Investigation of functional aspects of the N-terminal region of Escherichia coli T-protein of the glycine cleavage system

2000 ◽  
Vol 28 (5) ◽  
pp. A421-A421
Author(s):  
K. Okamura-Ikeda ◽  
K. Fujiwara ◽  
Y. Motokawa
Keyword(s):  
Escherichia Coli ◽  
Terminal Region ◽  
Glycine Cleavage System ◽  
Functional Aspects ◽  
Glycine Cleavage

scholarly journals Inhibition of glycine cleavage system by pyridoxine 5′‐phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli

2019 ◽  
Vol 113 (1) ◽  
pp. 270-284 ◽  
Author(s):  
Tomokazu Ito ◽  
Ran Hori ◽  
Hisashi Hemmi ◽  
Diana M. Downs ◽  
Tohru Yoshimura
Keyword(s):  
Escherichia Coli ◽  
Synthetic Lethality ◽  
Glycine Cleavage System ◽  
Glycine Cleavage

Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system

1993 ◽  
Vol 216 (2) ◽  
pp. 539-548 ◽  
Author(s):  
Kazuko OKAMURA-IKEDA ◽  
Yosuke OHMURA ◽  
Kazuko FUJIWARA ◽  
Yutaro MOTOKAWA
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Glycine Cleavage System ◽  
Glycine Cleavage ◽  
Gcv Operon

scholarly journals Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage

Viruses ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 90
Author(s):  
Denghui Yang ◽  
Zhaofei Wang ◽  
Jingjiao Ma ◽  
Qiang Fu ◽  
Lifei Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Receptor Protein ◽  
Type I ◽  
Glycine Cleavage System ◽  
Camp Receptor Protein ◽  
Regulatory Pathway ◽  
Camp Concentration ◽  
Methylene Tetrahydrofolate ◽  
Glycine Cleavage ◽  
Camp Receptor

The CRISPR/Cas system protects bacteria against bacteriophage and plasmids through a sophisticated mechanism where cas operon plays a crucial role consisting of cse1 and cas3. However, comprehensive studies on the regulation of cas3 operon of the Type I-E CRISPR/Cas system are scarce. Herein, we investigated the regulation of cas3 in Escherichia coli. The mutation in gcvP or crp reduced the CRISPR/Cas system interference ability and increased bacterial susceptibility to phage, when the casA operon of the CRISPR/Cas system was activated. The silence of the glycine cleavage system (GCS) encoded by gcvTHP operon reduced cas3 expression. Adding N5, N10-methylene tetrahydrofolate (N5, N10-mTHF), which is the product of GCS-catalyzed glycine, was able to activate cas3 expression. In addition, a cAMP receptor protein (CRP) encoded by crp activated cas3 expression via binding to the cas3 promoter in response to cAMP concentration. Since N5, N10-mTHF provides one-carbon unit for purine, we assumed GCS regulates cas3 through associating with CRP. It was evident that the mutation of gcvP failed to further reduce the cas3 expression with the crp deletion. These results illustrated a novel regulatory pathway which GCS and CRP co-regulate cas3 of the CRISPR/Cas system and contribute to the defence against invasive genetic elements, where CRP is indispensable for GCS regulation of cas3 expression.

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