LIV-1 breast cancer protein belongs to a new family of histidine-rich membrane proteins with potential to control intracellular zinc

2000 ◽  
Vol 28 (5) ◽  
pp. A394-A394
Author(s):  
K. M. Taylor ◽  
H. E. Morgan ◽  
R. I. Nicholson
Keyword(s):  
Breast Cancer ◽  
Membrane Proteins ◽  
Intracellular Zinc ◽  
New Family

scholarly journals Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epithelium

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ting Wu ◽  
Yinghua Wang ◽  
Tianxiong Xiao ◽  
Yirui Ai ◽  
Jinsong Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Membrane Proteins ◽  
Epithelial Cells ◽  
Adult Stem Cells ◽  
Vascular System ◽  
Negative Regulator ◽  
Breast Cancer Cell Lines ◽  
Screening System ◽  
Membrane Expression ◽  
Sorting Technique

Abstract Background Protein C receptor (Procr) has recently been shown to mark resident adult stem cells in the mammary gland, vascular system, and pancreatic islets. More so, high Procr expression was also detected and used as indicator for subsets of triple-negative breast cancers (TNBCs). Previous study has revealed Procr as a target of Wnt/β-catenin signaling; however, direct upstream regulatory mechanism of Procr remains unknown. To comprehend the molecular role of Procr during physiology and pathology, elucidating the upstream effectors of Procr is necessary. Here, we provide a system for screening negative regulators of Procr, which could be adapted for broad molecular analysis on membrane proteins. Results We established a screening system which combines CRISPR-Cas9 guided gene disruption with fluorescence activated cell sorting technique (FACS). CommaDβ (murine epithelial cells line) was used for the initial Procr upstream effector screening using lentiviral CRISPR-gRNA library. Shortlisted genes were further validated through individual lentiviral gRNA infection followed by Procr expression evaluation. Adam17 was identified as a specific negative inhibitor of Procr expression. In addition, MDA-MB-231 cells and Hs578T cells (human breast cancer cell lines) were used to verify the conserved regulation of ADAM17 over PROCR expression. Conclusion We established an efficient CRISPR-Cas9/FACS screening system, which identifies the regulators of membrane proteins. Through this system, we identified Adam17 as the negative regulator of Procr membrane expression both in mammary epithelial cells and breast cancer cells.

Leukocyte-mimicking nanovesicles for effective doxorubicin delivery to treat breast cancer and melanoma

2020 ◽  
Vol 8 (1) ◽  
pp. 333-341 ◽  
Author(s):  
Roberto Molinaro ◽  
Jonathan O. Martinez ◽  
Assaf Zinger ◽  
Alessandro De Vita ◽  
Gianluca Storci ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Membrane Proteins ◽  
Immune Cells ◽  
Inflammatory Pathway ◽  
Doxorubicin Delivery ◽  
Cancer Lesion ◽  
Treat Breast Cancer

Biomimetic nanovesicles deriving from leukocytes membrane proteins, called leukosomes, exhibit increased targeting of cancer vasculature and stroma by exploiting the inflammatory pathway responsible for recruiting immune cells to the cancer lesion.

scholarly journals ZIP7-Mediated Intracellular Zinc Transport Contributes to Aberrant Growth Factor Signaling in Antihormone-Resistant Breast Cancer Cells

Endocrinology ◽  
2008 ◽  
Vol 149 (10) ◽  
pp. 4912-4920 ◽  
Author(s):  
Kathryn M. Taylor ◽  
Petra Vichova ◽  
Nicola Jordan ◽  
Stephen Hiscox ◽  
Rhiannon Hendley ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Growth Factor Signaling ◽  
Zinc Transport ◽  
Intracellular Zinc

scholarly journals A new family of integral membrane proteins involved in transport of aromatic amino acids in Escherichia coli.

1991 ◽  
Vol 173 (10) ◽  
pp. 3231-3234 ◽  
Author(s):  
J P Sarsero ◽  
P J Wookey ◽  
P Gollnick ◽  
C Yanofsky ◽  
A J Pittard
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Membrane Proteins ◽  
Aromatic Amino Acids ◽  
Integral Membrane Proteins ◽  
New Family

Plasma membrane proteins: A new probe for the characterization of breast cancer

Life Sciences ◽  
2019 ◽  
Vol 234 ◽  
pp. 116777
Author(s):  
Heba M. Fahmy ◽  
Alaa M. Ismail ◽  
Amena S. El-Feky ◽  
Esraa S. Abu Serea ◽  
Wael M. Elshemey
Keyword(s):  
Breast Cancer ◽  
Plasma Membrane ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins

scholarly journals Subtype-specific accumulation of intracellular zinc pools is associated with the malignant phenotype in breast cancer

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Paige Chandler ◽  
Bose S. Kochupurakkal ◽  
Samina Alam ◽  
Andrea L. Richardson ◽  
David I. Soybel ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Malignant Phenotype ◽  
Intracellular Zinc ◽  
Specific Accumulation
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