An immunochemical study of the binding of blood coagulation proteins to artificial surfaces

2000 ◽  
Vol 28 (5) ◽  
pp. A362-A362
Author(s):  
B. J. Mion ◽  
B. F. Masterson
Keyword(s):  
Blood Coagulation ◽  
Immunochemical Study ◽  
Artificial Surfaces ◽  
Coagulation Proteins ◽  
Blood Coagulation Proteins

scholarly journals Protein Corona Gold Nanoparticles Fingerprinting Reveals a Profile of Blood Coagulation Proteins in the Serum of HER2-Overexpressing Breast Cancer Patients

2020 ◽  
Vol 21 (22) ◽  
pp. 8449
Author(s):  
María del Pilar Chantada-Vázquez ◽  
Antonio Castro López ◽  
María García-Vence ◽  
Benigno Acea-Nebril ◽  
Susana B. Bravo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Blood Coagulation ◽  
Correct Identification ◽  
Breast Cancer Patients ◽  
Luminal A ◽  
Her2 Positive ◽  
Luminal B ◽  
Coagulation Proteins ◽  
Blood Coagulation Proteins

Breast cancer (BC) is a molecularly heterogeneous disease that encompasses five major molecular subtypes (luminal A (LA), luminal B HER2 negative (LB-), luminal B HER2 positive (LB+), HER2 positive (HER2+) and triple negative breast cancer (TNBC)). BC treatment mainly depends on the identification of the specific subtype. Despite the correct identification, therapies could fail in some patients. Thus, further insights into the genetic and molecular status of the different BC subtypes could be very useful to improve the response of BC patients to the range of available therapies. In this way, we used gold nanoparticles (AuNPs, 12.96 ± 0.72 nm) as a scavenging tool in combination with Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH-MS) to quantitatively analyze the serum proteome alterations in the different breast cancer intrinsic subtypes. The differentially regulated proteins specific of each subtype were further analyzed with the bioinformatic tools STRING and PANTHER to identify the major molecular function, biological processes, cellular origin, protein class and biological pathways altered due to the heterogeneity in proteome of the different BC subtypes. Importantly, a profile of blood coagulation proteins was identified in the serum of HER2-overexpressing BC patients.

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