Dual functions of the N-terminal domain of S. pombe DNA ligase I

2000 ◽  
Vol 28 (5) ◽  
pp. A251-A251
Author(s):  
I. V. Martin ◽  
S. A. MacNeill
Keyword(s):  
Dna Ligase ◽  
Terminal Domain ◽  
Dna Ligase I ◽  
Dual Functions

scholarly journals The ligation of pol β mismatch insertion products governs the formation of promutagenic base excision DNA repair intermediates

2020 ◽  
Vol 48 (7) ◽  
pp. 3708-3721 ◽  
Author(s):  
Melike Çağlayan
Keyword(s):  
Excision Repair ◽  
Dna Ligase ◽  
Active Site Residues ◽  
Dna Ligases ◽  
Terminal Domain ◽  
Dna Ligase I ◽  
Nucleotide Insertion ◽  
Base Excision ◽  
Ligation Step

Abstract DNA ligase I and DNA ligase III/XRCC1 complex catalyze the ultimate ligation step following DNA polymerase (pol) β nucleotide insertion during base excision repair (BER). Pol β Asn279 and Arg283 are the critical active site residues for the differentiation of an incoming nucleotide and a template base and the N-terminal domain of DNA ligase I mediates its interaction with pol β. Here, we show inefficient ligation of pol β insertion products with mismatched or damaged nucleotides, with the exception of a Watson–Crick-like dGTP insertion opposite T, using BER DNA ligases in vitro. Moreover, pol β N279A and R283A mutants deter the ligation of the promutagenic repair intermediates and the presence of N-terminal domain of DNA ligase I in a coupled reaction governs the channeling of the pol β insertion products. Our results demonstrate that the BER DNA ligases are compromised by subtle changes in all 12 possible noncanonical base pairs at the 3′-end of the nicked repair intermediate. These findings contribute to understanding of how the identity of the mismatch affects the substrate channeling of the repair pathway and the mechanism underlying the coordination between pol β and DNA ligase at the final ligation step to maintain the BER efficiency.

scholarly journals The human checkpoint sensor and alternative DNA clamp Rad9–Rad1–Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery

2005 ◽  
Vol 389 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Ekaterina SMIRNOVA ◽  
Magali TOUEILLE ◽  
Enni MARKKANEN ◽  
Ulrich HÜBSCHER
Keyword(s):  
Quality Control ◽  
Dna Polymerase ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Dna Ligase ◽  
Dna Polymerase Β ◽  
Flap Endonuclease 1 ◽  
Important Target ◽  
Dna Ligase I ◽  
Base Excision

The human checkpoint sensor and alternative clamp Rad9–Rad1–Hus1 can interact with and specifically stimulate DNA ligase I. The very recently described interactions of Rad9–Rad1–Hus1 with MutY DNA glycosylase, DNA polymerase β and Flap endonuclease 1 now complete our view that the long-patch base excision machinery is an important target of the Rad9–Rad1–Hus1 complex, thus enhancing the quality control of DNA.

scholarly journals An N-terminal domain helical motif of Prototype Foamy Virus Gag with dual functions essential for particle egress and viral infectivity

Retrovirology ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 45 ◽  
Author(s):  
Juliane Reh ◽  
Annett Stange ◽  
Anne Götz ◽  
Marlene Rönitz ◽  
Arend Große ◽  
...  
Keyword(s):  
Foamy Virus ◽  
Viral Infectivity ◽  
Prototype Foamy Virus ◽  
Terminal Domain ◽  
Dual Functions

Cloning and sequence analysis of a cDNA coding for the murine DNA ligase I enzyme

Gene ◽  
1994 ◽  
Vol 144 (2) ◽  
pp. 253-257 ◽  
Author(s):  
Elena Savini ◽  
Giuseppe Biamonti ◽  
Giovanni Ciarrocchi ◽  
Alessandra Montecucco
Keyword(s):  
Sequence Analysis ◽  
Dna Ligase ◽  
Dna Ligase I

scholarly journals Dual mode of inhibition of purified DNA ligase I from human cells by 9-beta-D-arabinofuranosyl-2-fluoroadenine triphosphate.

1992 ◽  
Vol 267 (4) ◽  
pp. 2345-2349 ◽  
Author(s):  
S W Yang ◽  
P Huang ◽  
W Plunkett ◽  
F F Becker ◽  
J Y Chan
Keyword(s):  
Human Cells ◽  
Dna Ligase ◽  
Dual Mode ◽  
Dna Ligase I

scholarly journals Thermodynamics of Human DNA Ligase I Trimerization and Association with DNA Polymerase β

1998 ◽  
Vol 273 (32) ◽  
pp. 20540-20550 ◽  
Author(s):  
Emilios K. Dimitriadis ◽  
Rajendra Prasad ◽  
Mary K. Vaske ◽  
Ling Chen ◽  
Alan E. Tomkinson ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Ligase ◽  
Dna Polymerase Β ◽  
Human Dna ◽  
Dna Ligase I
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