Influence of the nucleotide excision repair on growth properties of E. coli harbouring triplet repeats containing plasmids

2000 ◽  
Vol 28 (5) ◽  
pp. A166-A166
Author(s):  
M. J. Majchrzak ◽  
M. P. Staczek ◽  
A. Jaworski ◽  
P. Parniewski
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Triplet Repeats ◽  
E Coli ◽  
Nucleotide Excision ◽  
Growth Properties

scholarly journals Processing closely spaced lesions during Nucleotide Excision Repair triggers mutagenesis in E. coli

PLoS Genetics ◽  
2017 ◽  
Vol 13 (7) ◽  
pp. e1006881 ◽  
Author(s):  
Régine Janel-Bintz ◽  
Rita L. Napolitano ◽  
Asako Isogawa ◽  
Shingo Fujii ◽  
Robert P. Fuchs
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
E Coli ◽  
Nucleotide Excision

Nucleotide excision repair: From E. coli to man

Biochimie ◽  
1999 ◽  
Vol 81 (1-2) ◽  
pp. 15-25 ◽  
Author(s):  
Claude Petit ◽  
Aziz Sancar
Keyword(s):  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
E Coli ◽  
Nucleotide Excision

The alkyltransferase-like ybaZ gene product enhances nucleotide excision repair of O6-alkylguanine adducts in E. coli

DNA Repair ◽  
2009 ◽  
Vol 8 (6) ◽  
pp. 697-703 ◽  
Author(s):  
Gerard Mazon ◽  
Gaëlle Philippin ◽  
Jean Cadet ◽  
Didier Gasparutto ◽  
Robert P. Fuchs
Keyword(s):  
Gene Product ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
E Coli ◽  
Nucleotide Excision

scholarly journals Interactions between yeast photolyase and nucleotide excision repair proteins in Saccharomyces cerevisiae and Escherichia coli

1989 ◽  
Vol 9 (11) ◽  
pp. 4767-4776
Author(s):  
G B Sancar ◽  
F W Smith
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Yeast Cells ◽  
E Coli ◽  
Pyrimidine Dimers ◽  
Nucleotide Excision ◽  
Dark Survival

The PHR1 gene of Saccharomyces cerevisiae encodes a DNA photolyase that catalyzes the light-dependent repair of pyrimidine dimers. In the absence of photoreactivating light, this enzyme binds to pyrimidine dimers but is unable to repair them. We have assessed the effect of bound photolyase on the dark survival of yeast cells carrying mutations in genes that eliminate either nucleotide excision repair (RAD2) or mutagenic repair (RAD18). We found that a functional PHR1 gene enhanced dark survival in a rad18 background but failed to do so in a rad2 or rad2 rad18 background and therefore conclude that photolyase stimulates specifically nucleotide excision repair of dimers in S. cerevisiae. This effect is similar to the effect of Escherichia coli photolyase on excision repair in the bacterium. However, despite the functional and structural similarities between yeast photolyase and the E. coli enzyme and complementation of the photoreactivation deficiency of E. coli phr mutants by PHR1, yeast photolyase failed to enhance excision repair in the bacterium. Instead, Phr1 was found to be a potent inhibitor of dark repair in recA strains but had no effect in uvrA strains. The results of in vitro experiments indicate that inhibition of nucleotide excision repair results from competition between yeast photolyase and ABC excision nuclease for binding at pyrimidine dimers. In addition, the A and B subunits of the excision nuclease, when allowed to bind to dimers before photolyase, suppressed photoreactivation by Phr1. We propose that enhancement of nucleotide excision repair by photolyases is a general phenomenon and that photolyase should be considered an accessory protein in this pathway.

scholarly journals The effect of essential oil of basil (Ocimum basilicum L.) on UV-induced mutagenesis in Escherichia coli and Saccharomyces cerevisiae

2008 ◽  
Vol 60 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Jasna Stanojevic ◽  
Tanja Beric ◽  
Biljana Opacic ◽  
Branka Vukovic-Gacic ◽  
Draga Simic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Ocimum Basilicum ◽  
Major Constituent ◽  
Spontaneous Mutagenesis ◽  
E Coli ◽  
Antimutagenic Effect ◽  
Nucleotide Excision ◽  
Induced Mutagenesis

The antimutagenic potential of essential oil (EO) of basil (Ocimum basilicum L.) and its major constituent linalool were studied with the E. coli K12 and S.cerevisiae D7 assays. In the E. coli assay, EO and linalool inhibited UV-induced mutagenesis in a repair-proficient strain, but had no effect on spontaneous mutagenesis in repair-proficient, nucleotide excision repair-deficient, and mismatch-deficient strains. By testing participation of different mechanisms involved in antimutagenesis, it was concluded that the antimutagenic effect against UV-induced mutagenesis involved decrease of protein synthesis and cell proliferation which led to increased efficiency of nucleotide excision repair. An antimutagenic effect of basil derivatives in S. cerevisiae was not detected.

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