Towards the cloning of GLY1

2000 ◽  
Vol 28 (6) ◽  
pp. 675-677 ◽  
Author(s):  
M. Miquel
Keyword(s):  
Carbon Flux ◽  
Biochemical Study ◽  
Complete Sequence ◽  
Chromosome Walking ◽  
Ethyl Methane Sulphonate ◽  
Methane Sulphonate ◽  
Glycerol Treatment ◽  
Phosphate Supply ◽  
Chromosome Ii ◽  
Map Location

The Arabidopsis mutants designated gly1 exhibit a reduced carbon flux through the prokaryotic pathway that is compensated for by an increased carbon flux through the eukaryotic pathway. Biochemical approaches reveal that the gly1 phenotype cannot be explained by a deficiency in the enzymes of the prokaryotic pathway. The chemical complementation of the mutant phenotype by exogenous glycerol treatment of gly1 plants suggests a lesion affecting the glycerol 3-phosphate supply within the chloroplast. As an alternative to the biochemical study of the gly1 mutants we set out to map the GLY1 locus. The gly1 mutant being an EMS (ethyl methane sulphonate) mutant, we used a strategy based on the polymorphism existing between Arabidopsis ecotypes, here Columbia (gly1 background) and Landsberg erecta. We mapped gly1 on chromosome II. During the process of chromosome walking, the complete sequence of chromosome II was released, allowing us to make assumptions on candidate genes based on map location. We are currently sequencing the putative genes.

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

Evaluation of Yield Attributing Variants Developed Through Ethyl Methane Sulphonate in an Important Proteinaceous Crop-Vicia faba

2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam
Keyword(s):  
Vicia Faba ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

scholarly journals Screening for pod shattering in mutant population of mungbean (Vigna radiata (L.) Wilczek)

2017 ◽  
Vol 9 (3) ◽  
pp. 1787-1791 ◽  
Author(s):  
N. Vairam ◽  
S. Anandhi Lavanya ◽  
C. Vanniarajan
Keyword(s):  
Gamma Rays ◽  
Vigna Radiata ◽  
Visual Observation ◽  
Induced Mutations ◽  
Ethyl Methane Sulphonate ◽  
Pod Shattering ◽  
Indian Agriculture ◽  
Methane Sulphonate ◽  
Important Objective ◽  
Pod Dehiscence

Mungbean, (Vigna radiata (L.) Wilczek) occupies a unique position in Indian agriculture and has been grown under various agro-ecological conditions. It is cultivated in 1.61mha with production of 3.38MT and productivi-ty of 474kg/ha in India. Mungbean pods are thin and brittle when dry, so shattering is a major problem. The loss of seeds by pod dehiscence is one of the major reasons for low yield in mungbean; thus, reducing the frequency of pod dehiscence is an important objective in mungbean breeding. Induced mutations, have offered a single and short alternative to conventional breeding including isolation, screening, selection and testing generation after generation. In this study, variability was induced by gamma rays and Ethyl methane sulphonate (EMS) in two greengram geno-types viz., CO (Gg) 7 and NM 65. Screening for pod shattering was carried out in M2 and M3 populations of green-gram. The scoring for shattering was recorded at physiological maturity of the pod. The shattering percentage ranged from 14.56 (400 Gy) to 93.45 per cent (20 mM). A total of 100 shattering tolerant mutants were selected from field based on visual observation. These mutants were again scored under laboratory condition as per IITA method. A total of 12 mutants of CO (Gg) 7 and 10 mutants of NM 65 which were tolerant to pod shattering were identified in M2 generation and forwarded to M3 generation. These mutants were scored for pod shattering under laboratory con-dition and nine mutants viz., M26, M44, M46, M58, M70, M71, M84, M92 and M98 were found to be tolerant in M3 generation. This study on identification and screening of the mutants tolerant to pod shattering with high yielding potential will help to increase the production of the pods to a greater extent.

scholarly journals Induced Mutagenesis Enhances Lodging Resistance and Photosynthetic Efficiency of Kodomillet (Paspalum Scrobiculatum)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 227 ◽  
Author(s):  
James Poornima Jency ◽  
Ravikesavan Rajasekaran ◽  
Roshan Kumar Singh ◽  
Raveendran Muthurajan ◽  
Jeyakumar Prabhakaran ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Ethyl Methane Sulphonate ◽  
Lodging Resistance ◽  
Pyruvate Phosphate Dikinase ◽  
Photosynthetic System ◽  
Methane Sulphonate ◽  
Induced Mutagenesis

The present research was focused in the development of photosynthetically efficient (PhE) and non-lodging mutants by utilizing ethyl methane sulphonate (EMS) and gamma radiation in the kodomillet variety CO 3, prone to lodging. Striking variations in a number of anatomical characteristics of leaf anatomy for PhE and culm thickness for lodging resistance was recorded in M2 (second mutant) generation. The identified mutants were subjected to transcriptomic studies to understand their molecular basis. Expression profiling was undertaken for pyruvate phosphate dikinase (PPDK), Nicotinamide Adenine Dinucleotide Phosphate Hydrogen—(NADPH) and NADP-dependent malate dehydrogenase (NADP-MDH) in the mutants CO 3-100-7-12 (photosynthetically efficient) and in CO 3-200-13-4 (less efficient). For lodging trait, two mutants CO 3-100-18-22 (lodged) and CO 3-300-7-4 (non-lodged) were selected for expression profiling using genes GA2ox6 and Rht-B. The studies confirmed the expression of PPDK increased 30-fold, NADP-ME2 ~1-fold and NADP-MDH10 was also highly expressed in the mutant CO 3-100-7-12. These expression profiles suggest that kodomillet uses an NADP-malic enzyme subtype C4 photosynthetic system. The expression of Rht-B was significantly up regulated in CO 3-300-7-4. The study highlights the differential expression patterns of the same gene in different lines at different time points of stress as well as non-stress conditions. This infers that the mutation has some effect on their expression; otherwise the expression levels will be unaltered. Enhancement in grain yield could be best achieved by developing a phenotype with high PhE and culm with thick sclerenchyma cells.

Effect of seed treatment by ethyl methane sulphonate on growth, flowering and yield of papaya cv. Pusa Dwarf

2016 ◽  
Vol 7 (1) ◽  
pp. 64
Author(s):  
Mahesh Kumar ◽  
Mukesh Kumar ◽  
Satya Prakash ◽  
DK Gautam ◽  
Sanjeev Rao
Keyword(s):  
Seed Treatment ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

The complete sequence of a 19,482 bp segment located on the right arm of Chromosome II fromSaccharomyces cerevisiae

Yeast ◽  
1993 ◽  
Vol 9 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Marc Crouzet ◽  
Michel Aigle
Keyword(s):  
Complete Sequence ◽  
The Right ◽  
Chromosome Ii

scholarly journals Increased sensitivity to cell killing and mutagenesis by chemical mutagens in thymidine-kinase-deficient subclones of a Friend murine leukaemia cell line

1982 ◽  
Vol 40 (2) ◽  
pp. 207-212 ◽  
Author(s):  
P. G. McKenna ◽  
A. A. Yasseen
Keyword(s):  
Thymidine Kinase ◽  
Wild Type ◽  
Ethyl Methane Sulphonate ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Methane Sulphonate ◽  
Increased Sensitivity ◽  
Unit Dose

SUMMARYWild-type Friend murine leukaemia (clone 707) cells and two thymidinekinase-deficient subclones, 707BUE and 707BUF, were compared for sensitivity to killing and mutagenesis by the chemical mutagens, ethyl methane sulphonate (EMS),N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), and methyl methane sulphonate (MMS). The two thymidine-kinase-deficient subclones were significantly more sensitive to killing by each of the four chemical mutagens than were wild-type cells. The increased sensitivity to killing by the four mutagens was also reflected in increased mutagenesis (per unit dose of mutagen) to 6-thioguanine resistance. In the light of these results, the significance of thymidine kinase in DNA repair and mutagenesis is discussed.

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