Effects of manipulating expression of acetyl-CoA carboxylase I in Brassica napus L. embryos

2000 ◽  
Vol 28 (6) ◽  
pp. 598-600 ◽  
Author(s):  
C. Sellwood ◽  
A. R. Slabas ◽  
S. Rawsthorne
Keyword(s):  
Brassica Napus ◽  
Carbohydrate Metabolism ◽  
Oilseed Rape ◽  
Lipid Content ◽  
Seed Lipid ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Brassica Napus L ◽  
Carboxylase Activity ◽  
Antisense Expression

Acetyl-CoA carboxylase I (ACCase I) in developing oilseed rape embryos is predominantly cystosolic, based upon measurement of its propionyl-CoA carboxylase activity. Reduction of ACCase I by antisense expression reduces seed lipid content and affects carbohydrate metabolism.

scholarly journals Significant Reductions in Oil Quality and Lipid Content of Oilseed Rape (Brassica Napus L.) Under Climate Change

2015 ◽  
Vol 29 ◽  
pp. 121-122 ◽  
Author(s):  
Shahla Namazkar ◽  
Helge Egsgaard ◽  
Georg Frenck ◽  
Thilde Terkelsen ◽  
Rikke Bagger Jørgensen
Keyword(s):  
Climate Change ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Lipid Content ◽  
Oil Quality ◽  
Brassica Napus L

scholarly journals Biotin carboxyl carrier protein and carboxyltransferase subunits of the multi-subunit form of acetyl-CoA carboxylase from Brassica napus: cloning and analysis of expression during oilseed rape embryogenesis

1996 ◽  
Vol 315 (1) ◽  
pp. 103-112 ◽  
Author(s):  
Kieran M. ELBOROUGH ◽  
Robert WINZ ◽  
Ranjit K. DEKA ◽  
Jonathan E. MARKHAM ◽  
Andrew J. WHITE ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Embryo Development ◽  
Oilseed Rape ◽  
Northern Analysis ◽  
Carrier Protein ◽  
Type I ◽  
Type Ii ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Biotin Carboxyl Carrier Protein

In the oilseed rape Brassica napus there are two forms of acetyl-CoA carboxylase (ACCase). As in other dicotyledonous plants there is a type I ACCase, the single polypeptide 220 kDa form, and a type II multi-subunit complex analogous to that of Escherichia coli and Anabaena. This paper describes the cloning and characterization of a plant biotin carboxyl carrier protein (BCCP) from the type II ACCase complex that shows 61% identity/79% similarity with Anabaena BCCP at the amino acid level. Six classes of nuclear encoded oilseed rape BCCP cDNA were cloned, two of which contained the entire coding region. The BCCP sequences allowed the assignment of function to two previously unassigned Arabidopsis expressed sequence tag (EST) sequences. We also report the cloning of a second type II ACCase component from oilseed rape, the β-carboxyltransferase subunit (βCT), which is chloroplast-encoded. Northern analysis showed that although the relative levels of BCCP and βCT mRNA differed between different oilseed rape tissues, their temporal patterns of expression were identical during embryo development. At the protein level, expression of BCCP during embryo development was studied by Western blotting, using affinity-purified anti-biotin polyclonal sera. With this technique a 35 kDa protein thought to be BCCP was shown to reside within the chloroplast. This analysis also permitted us to view the differential expression of several unidentified biotinylated proteins during embryogenesis.

scholarly journals Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

2020 ◽  
Vol 21 (22) ◽  
pp. 8740
Author(s):  
Daria Chlebek ◽  
Artur Pinski ◽  
Joanna Żur ◽  
Justyna Michalska ◽  
Katarzyna Hupert-Kocurek
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Oilseed Rape ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Genome Mining ◽  
Plant Colonization ◽  
Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions

Planta ◽  
2004 ◽  
Vol 221 (3) ◽  
pp. 328-338 ◽  
Author(s):  
Jens Tilsner ◽  
Nina Kassner ◽  
Christine Struck ◽  
Gertrud Lohaus
Keyword(s):  
Amino Acid ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Amino Acid Contents ◽  
Nitrogen Conditions

scholarly journals Critical phosphorylation sites for acetyl-CoA carboxylase activity.

1994 ◽  
Vol 269 (35) ◽  
pp. 22162-22168 ◽  
Author(s):  
J. Ha ◽  
S. Daniel ◽  
S.S. Broyles ◽  
K.H. Kim
Keyword(s):  
Phosphorylation Sites ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Carboxylase Activity

Effects of abscisic acid and temperature on erucic acid accumulation in oilseed rape (Brassica napus L.)

1997 ◽  
Vol 150 (4) ◽  
pp. 414-419 ◽  
Author(s):  
Jeroen A. Wilmer ◽  
Johannes P.F.G. Helsper ◽  
Linus H.W. van der Plas
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Oilseed Rape ◽  
Brassica Napus L ◽  
Acid Accumulation
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