NF-κB activation in single living cells — Analysis of anti-apoptosis and kinetics of activation by IL-1β

1999 ◽  
Vol 27 (3) ◽  
pp. A94-A94
Author(s):  
Franço Carlotti ◽  
Lin Yang ◽  
Steven K. Dower ◽  
Eva E. Qwarnstrom
Keyword(s):  
Living Cells ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

scholarly journals The Rate of Proflavin Passage into Single Living Cells with Application to Permeability Studies

1960 ◽  
Vol 43 (4) ◽  
pp. 853-866 ◽  
Author(s):  
Elliott Robbins
Keyword(s):  
Tissue Culture ◽  
Physicochemical Properties ◽  
Single Cell ◽  
Uptake Rate ◽  
Living Cells ◽  
Dye Transport ◽  
Permeability Studies ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

A technique for the measurement of the uptake rate of proflavin by the single cell in tissue culture has been developed, and the kinetics of the dye transport are discussed in terms of its physicochemical properties. Some applications of the technique to the study of permeability are given.

scholarly journals Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells

2012 ◽  
Vol 4 (10) ◽  
pp. 846-853 ◽  
Author(s):  
Wei Wang ◽  
Yunze Yang ◽  
Shaopeng Wang ◽  
Vinay J. Nagaraj ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Living Cells ◽  
Binding Kinetics ◽  
Label Free ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

scholarly journals Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hideaki Ando ◽  
Matsumi Hirose ◽  
Gen Kurosawa ◽  
Soren Impey ◽  
Katsuhiko Mikoshiba
Keyword(s):  
Time Lapse ◽  
Living Cells ◽  
Single Living Cells ◽  
Time Lapse Imaging ◽  
Kinetics Of ◽  
Single Living

scholarly journals Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shan-Shan Li ◽  
Qi-Yuan Guan ◽  
Gang Meng ◽  
Xiao-Feng Chang ◽  
Ji-Wu Wei ◽  
...  
Keyword(s):  
Living Cells ◽  
Drug Action ◽  
Chemical Processes ◽  
Single Living Cells ◽  
Kinetics Of ◽  
Single Living

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

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