25 Role of isoprenylation in the inhibitory action of lovastatin on proliferation of SV40 immortalized human saphenous vein smooth muscle cells

1998 ◽  
Vol 26 (4) ◽  
pp. S324-S324
Author(s):  
SHEBNEM UNLU ◽  
CATHERINE D. MASON ◽  
ALUN D. HUGHES
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Saphenous Vein ◽  
Inhibitory Action ◽  
Muscle Cells ◽  
Human Saphenous Vein

scholarly journals Cross-talk between angiotensin II and IGF-1-induced connexin 43 expression in human saphenous vein smooth muscle cells

2011 ◽  
Vol 15 (8) ◽  
pp. 1695-1702 ◽  
Author(s):  
Guanghong Jia ◽  
Anshu Aggarwal ◽  
Amanuel Yohannes ◽  
Deepak M. Gangahar ◽  
Devendra K. Agrawal
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Smooth Muscle Cells ◽  
Cross Talk ◽  
Saphenous Vein ◽  
Connexin 43 ◽  
Muscle Cells ◽  
Human Saphenous Vein

Effect of recombinant growth factors on human saphenous vein smooth muscle cells

1998 ◽  
Vol 34 (6) ◽  
pp. 431-433 ◽  
Author(s):  
Paul Dunlop ◽  
Karen Porter ◽  
Kevin Varty ◽  
Peter Bell ◽  
Nicholas London
Keyword(s):  
Smooth Muscle ◽  
Growth Factors ◽  
Smooth Muscle Cells ◽  
Saphenous Vein ◽  
Muscle Cells ◽  
Human Saphenous Vein

Cyclic stretch-induced Crp3 sensitizes vascular smooth muscle cells to apoptosis during vein arterialization remodeling

2018 ◽  
Vol 132 (4) ◽  
pp. 449-459
Author(s):  
Luciene Cristina Gastalho Campos ◽  
João Carlos Ribeiro-Silva ◽  
Alessandra Santos Menegon ◽  
Valerio Garrone Barauna ◽  
Ayumi Aurea Miyakawa ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Saphenous Vein ◽  
Caspase 3 ◽  
Jugular Vein ◽  
Muscle Cells ◽  
Cyclic Stretch ◽  
Effector Caspase ◽  
Induced Apoptosis

Vein graft failure limits the long-term patency of the saphenous vein used as a conduit for coronary artery bypass graft. Early graft adaptation involves some degree of intima hyperplasia to sustain the hemodynamic stress, but the progress to occlusion in some veins remains unclear. We have demonstrated that stretch-induced up-regulation of cysteine and glycine-rich protein 3 (Crp3) in rat jugular vein and human saphenous vein in response to arterialization. Here, we developed a Crp3-knockout (KO) rat to investigate the role of Crp3 in vascular remodeling. After 28 days of jugular vein arterialization, the intima layer was three-fold thicker in the Crp3-KO that showed comparable smooth muscle cells (SMC) proliferation but an absence of early apoptosis observed in the wild-type (WT) rat. We then investigated the role of Crp3 in early integrin-mediated signaling apoptosis in isolated jugular SMC. Interestingly, under basal conditions, ceramide treatment failed to induce apoptosis in both WT and Crp3-KO SMC. Under stretch, Crp3 expression increased in WT SMC and ceramide-induced apoptosis. Immunoblotting analysis indicated that ceramide stretch-induced apoptosis in SMC is accompanied by a decrease in the phosphorylation status of both focal adhesion kinase (Fak) and protein kinase B (Akt), leading to an increase in Bax expression and caspase-3 cleavage. In contrast, ceramide failed to decrease Fak and Akt phosphorylation in Crp3-KO SMC and, therefore, there was no downstream induction of Bax expression and effector caspase-3 cleavage. Taken together, we provide evidence that stretch-induced Crp3 modulates vein remodeling in response to arterialization by sensitizing SMC to apoptosis.

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