Kinetics of Reactive Oxygen Intermediate Formation and Apoptosis in Human Glioma and Glioma C6 Cell Line: Effects of Radiation and n-6 Essential Fatty Acids

1998 ◽  
Vol 26 (2) ◽  
pp. S141-S141
Author(s):  
JR WILLIAMS ◽  
HA LEAVER ◽  
JW IRONSIDE ◽  
A GREGOR ◽  
E MILLER ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cell Line ◽  
Essential Fatty Acids ◽  
Intermediate Formation ◽  
Reactive Oxygen Intermediate ◽  
Human Glioma ◽  
Glioma C6 ◽  
Effects Of Radiation ◽  
Kinetics Of ◽  
C6 Cell Line

scholarly journals Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids

2013 ◽  
Vol 48 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Róberson Sakabe ◽  
Flávio Ruas de Moraes ◽  
Marco Antonio de Andrade Belo ◽  
Fabiana Pilarski ◽  
Julieta Rodini Engrácia de Moraes
Keyword(s):  
Fatty Acids ◽  
Growth Performance ◽  
Giant Cell ◽  
Nile Tilapia ◽  
Linseed Oil ◽  
Essential Fatty Acids ◽  
Cell Formation ◽  
Giant Cell Formation ◽  
Kinetics Of ◽  
Macrophage Accumulation

The objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus). The supplementation sources were soybean oil (SO, source of omega 6, n‑6) and linseed oil (LO, source of omega 3, n‑3), in the following proportions: 100% SO; 75% SO + 25% LO; 50% SO + 50% LO; 25% SO + 75% LO; and 100% LO (four replicates per treatment). After a feeding period of three months, growth performance was evaluated, and glass coverslips were implanted into the subcutaneous connective tissue of fish, being removed for examination at 2, 4, 6, and 8 days after implantation. Growth performance did not differ between treatments. Fish fed 100% linseed oil diet had the greatest macrophage accumulation and the fastest Langhans cell formation on the sixth day. On the eighth day, Langhans cells were predominant on the coverslips implanted in the fish feed 75 and 100% linseed oil. n‑3 fatty acids may contribute to macrophage recruitment and giant cell formation in fish chronic inflammatory response to foreign body.

scholarly journals P2Y1 nucleotide receptor silencing and its effect on glioma C6 calcium signaling.

2012 ◽  
Vol 59 (4) ◽  
Author(s):  
Dorota Wypych ◽  
Pawel Pomorski
Keyword(s):  
Calcium Signaling ◽  
Cell Line ◽  
Serum Deprivation ◽  
Serum Starvation ◽  
Nucleotide Receptors ◽  
Akt Phosphorylation ◽  
Glioma C6 ◽  
Protein Change ◽  
C6 Cell Line

In our earlier studies of the signaling cross-talk between nucleotide receptors in an in vitro glioma model (C6 cell line) under prolonged serum deprivation conditions, a growth arrest of the cells and expression shift from P2Y(1) to P2Y(12) receptors was found. The aim of the present work was to test if siRNA silencing of P2Y(1) receptor changes P2Y(12) expression similarly as following the serum deprivation and which physiological downstream pathways it affects. Here we demonstrate for the first time the efficiency of siRNA technology in silencing P2Y nucleotide receptors in glioma C6 cell line. Moreover, P2Y(12) proved to be insensitive to the P2Y(1) receptor silencing. The effect of the P2Y(1) silencing on calcium signaling was less pronounced then the extent of the protein change itself, exactly as was the case for the serum starvation experiments. Phosphorylation of ERK and Akt kinases were studied as the downstream effect of P2Y(1)-evoked signaling and similar effects as in the case of serum deprivation were found for ERK, and even stronger ones for Akt phosphorylation.

Neonatal nutritional strategy of a viviparous elasmobranch with extremely low reproductive output

2020 ◽  
Vol 638 ◽  
pp. 107-121 ◽  
Author(s):  
BS Rangel ◽  
NE Hussey ◽  
Y Niella ◽  
LA Martinelli ◽  
AD Gomes ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Reproductive Strategies ◽  
Trophic Ecology ◽  
Essential Fatty Acids ◽  
Physiological State ◽  
Maternal Investment ◽  
Early Life History ◽  
Nutritional State ◽  
Disc Width ◽  
Biomarker Analysis

Throughout evolutionary history, elasmobranchs have developed diverse reproductive strategies. Little focused work, however, has addressed how neonatal nutritional state is affected by differing degrees of maternal investment associated with these markedly different reproductive strategies. To investigate the effect of maternal investment on the nutritional quality of pups during the early life history of an extremely viviparous elasmobranch, quantitative biomarker analysis including lipids, fatty acids and stable isotopes was conducted. Using the cownose ray Rhinoptera bonasus (histotrophic viviparous) as a model, we found that pups were initially born in a positive nutritional state, enriched in physiologically important essential fatty acids and nitrogen and carbon stable isotope values (δ15N and δ13C), a result of maternal intrauterine transfer. A systematic decrease in some fatty acids and δ15N values, as well as a decrease in cholesterol with growth, confirmed that these substrates were derived from maternal resources and used in initial metabolic processes following birth. An observed increase in condition factor, plasma essential fatty acids and triglyceride:cholesterol ratio with increasing body size identified a progression towards successful independent foraging with pups not displaying marked nutritional deficiency or fasting phases. Our multi-tracer approach allowed the identification of 2 size classes of young rays (<50 and <70 cm disc width) that displayed distinct physiological states. Since prenatal maternal investment is critical for offspring condition and to promote successful foraging post birth, understanding the trophic ecology and physiological state of pups during their first year is critical to guide management and conservation within nursery grounds.

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