90 Report on a longitudinal study of hprt mutant frequency in vivo in a group of healthy humans

1998 ◽  
Vol 26 (1) ◽  
pp. S76-S76 ◽  
Author(s):  
CAROLINE A. WARNOCK ◽  
M. BARBARA E. LIVINGSTONE ◽  
EDWARD S. GILLESPIE ◽  
CHRISTOPHER R. BARNETT ◽  
YVONNE A. BARNETT
Keyword(s):  
Longitudinal Study ◽  
Healthy Humans ◽  
Mutant Frequency

Physical exercise increases autophagic signaling through ULK1 in human skeletal muscle

2015 ◽  
Vol 118 (8) ◽  
pp. 971-979 ◽  
Author(s):  
Andreas Buch Møller ◽  
Mikkel Holm Vendelbo ◽  
Britt Christensen ◽  
Berthil Forrest Clasen ◽  
Ann Mosegaard Bak ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Physical Exercise ◽  
Light Chain ◽  
Human Skeletal Muscle ◽  
Transgenic Animal ◽  
Dependent Manner ◽  
Healthy Humans ◽  
Transgenic Animal Models ◽  
Exercise Induced

Data from transgenic animal models suggest that exercise-induced autophagy is critical for adaptation to physical training, and that Unc-51 like kinase-1 (ULK1) serves as an important regulator of autophagy. Phosphorylation of ULK1 at Ser555 stimulates autophagy, whereas phosphorylation at Ser757 is inhibitory. To determine whether exercise regulates ULK1 phosphorylation in humans in vivo in a nutrient-dependent manner, we examined skeletal muscle biopsies from healthy humans after 1-h cycling exercise at 50% maximal O2 uptake on two occasions: 1) during a 36-h fast, and 2) during continuous glucose infusion at 0.2 kg/h. Physical exercise increased ULK1 phosphorylation at Ser555 and decreased lipidation of light chain 3B. ULK1 phosphorylation at Ser555 correlated positively with AMP-activated protein kinase-α Thr172 phosphorylation and negatively with light chain 3B lipidation. ULK1 phosphorylation at Ser757 was not affected by exercise. Fasting increased ULK1 and p62 protein expression, but did not affect exercise-induced ULK1 phosphorylation. These data demonstrate that autophagy signaling is activated in human skeletal muscle after 60 min of exercise, independently of nutritional status, and suggest that initiation of autophagy constitutes an important physiological response to exercise in humans.

scholarly journals The influence of environmental and core temperature on cyclooxygenase and PGE2 in healthy humans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Christopher J. Esh ◽  
Bryna C. R. Chrismas ◽  
Alexis R. Mauger ◽  
Anissa Cherif ◽  
John Molphy ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Skin Temperature ◽  
Effect Size ◽  
Mixed Models ◽  
Thermal Perception ◽  
Random Coefficient Models ◽  
Healthy Humans ◽  
Within Subjects ◽  
Thermogenic Response

AbstractWhether cyclooxygenase (COX)/prostaglandin E2 (PGE2) thermoregulatory pathways, observed in rodents, present in humans? Participants (n = 9) were exposed to three environments; cold (20 °C), thermoneutral (30 °C) and hot (40 °C) for 120 min. Core (Tc)/skin temperature and thermal perception were recorded every 15 min, with COX/PGE2 concentrations determined at baseline, 60 and 120 min. Linear mixed models identified differences between and within subjects/conditions. Random coefficient models determined relationships between Tc and COX/PGE2. Tc [mean (range)] increased in hot [+ 0.8 (0.4–1.2) °C; p < 0.0001; effect size (ES): 2.9], decreased in cold [− 0.5 (− 0.8 to − 0.2) °C; p < 0.0001; ES 2.6] and was unchanged in thermoneutral [+ 0.1 (− 0.2 to 0.4) °C; p = 0.3502]. A relationship between COX2/PGE2 in cold (p = 0.0012) and cold/thermoneutral [collapsed, condition and time (p = 0.0243)] was seen, with higher PGE2 associated with higher Tc. A within condition relationship between Tc/PGE2 was observed in thermoneutral (p = 0.0202) and cold/thermoneutral [collapsed, condition and time (p = 0.0079)] but not cold (p = 0.0631). The data suggests a thermogenic response of the COX/PGE2 pathway insufficient to defend Tc in cold. Further human in vivo research which manipulates COX/PGE2 bioavailability and participant acclimation/acclimatization are warranted to elucidate the influence of COX/PGE2 on Tc.

An analysis of in vivo hprt mutant frequency in circulating T-lymphocytes in the normal human population: a comparison of four datasets

1994 ◽  
Vol 313 (2-3) ◽  
pp. 227-247 ◽  
Author(s):  
Derek R. Robinson ◽  
Kevin Goodall ◽  
Richard J. Albertini ◽  
J.Patrick O'Neill ◽  
Barry Finette ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Human Population ◽  
Normal Human ◽  
Mutant Frequency

scholarly journals Inside the Decentralised Casino: A Longitudinal Study of Actual Cryptocurrency Gambling Transactions

2020 ◽  
Author(s):  
Oliver James Scholten ◽  
David Zendle ◽  
James Alfred Walker
Keyword(s):  
Data Analysis ◽  
Longitudinal Study ◽  
Large Scale ◽  
Online Gambling ◽  
Research Platform ◽  
Player Behaviour ◽  
Typical User

Decentralised gambling applications are a new way for individuals to engage in online gambling. Decentralised gambling applications are distinguished from traditional online casinos in that individuals use cryptocurrency as a stake. Furthermore, rather than being stored on a traditional server, decentralised gambling applications are stored on a cryptocurrency’s blockchain.Previous work in the player behaviour tracking literature has examined the spending profiles of gamblers on traditional online casinos. However, parallel work has not taken place in the decentralised gambling domain. The profile of gamblers on decentralised gambling applications are therefore not known.This paper explores 2,232,741 transactions from 24,234 unique addresses to three such applications operating atop the Ethereum cryptocurrency network over 583 days. We present spending profiles across these applications, providing the first detailed summary of spending behaviours in this technologically advanced domain. We find that the typical user spends approximately \$110 equivalent across a median of 6 bets in a single day, although heavily involved bettors spend approximately \$100,000 equivalent over a median of 644 bets across 35 days. Our findings suggest that the use of decentralised gambling applications typically involves lower and less frequent expenditures than other online casinos, but that the most heavily involved players in this new domain spend substantially more. Our findings also demonstrate the use of these applications as a research platform, specifically for large scale longitudinal in-vivo data analysis.

P.107 Common HTR2A variants and 5-HTTLPR are not associated with in-vivo serotonin 2A receptor binding in healthy humans

2020 ◽  
Vol 31 ◽  
pp. S6-S7
Author(s):  
M. Spies ◽  
A. Nasser ◽  
B. Ozenne ◽  
P.S. Jensen ◽  
G.M. Knudsen ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Serotonin 2A Receptor ◽  
Healthy Humans ◽  
Serotonin 2A

Rifaximin in Chronic Liver Disease-induced Hepatic Encephalopathy: An In Vivo Longitudinal Study of Brain Metabolism on BDL Rats

2017 ◽  
Vol 7 ◽  
pp. S55-S56
Author(s):  
Emmanuelle Flatt ◽  
Cristina Cudalbu ◽  
Olivier Braissant ◽  
Stefan Mitrea ◽  
Dario Sessa ◽  
...  
Keyword(s):  
Liver Disease ◽  
Longitudinal Study ◽  
Hepatic Encephalopathy ◽  
Chronic Liver Disease ◽  
Brain Metabolism ◽  
Chronic Liver

scholarly journals The Variability of Translocator Protein Signal in Brain and Blood of Genotyped Healthy Humans Using In Vivo 123 I-CLINDE SPECT Imaging: A Test–Retest Study

2016 ◽  
Vol 58 (6) ◽  
pp. 989-995 ◽  
Author(s):  
Ling Feng ◽  
Per Jensen ◽  
Gerda Thomsen ◽  
Agnete Dyssegaard ◽  
Claus Svarer ◽  
...  
Keyword(s):  
Spect Imaging ◽  
Translocator Protein ◽  
Healthy Humans

Surface Parameterization of an Abdominal Aortic Aneurysm to Characterize Geometrical Evolution During Longitudinal Study of Patients

2012 ◽  
Author(s):  
A. Dupay ◽  
P. Snyder ◽  
W. Lee ◽  
S. Baek
Keyword(s):  
Abdominal Aortic Aneurysm ◽  
Longitudinal Study ◽  
Aortic Aneurysm ◽  
Disease Progression ◽  
3D Models ◽  
Aortic Aneurysms ◽  
Surrounding Tissue ◽  
Geometrical Parameters ◽  
Abdominal Aortic

For an abdominal aortic aneurysm (AAA) in vivo there are multiple tissues contacting its boundary, none of which have been fully considered for their effect throughout disease progression. Trends such as arterial asymmetry, surface curvature flattening, and arterial tortuosity could be significantly influenced by both surrounding tissue and hemodynamic factors. In order to quantify either the combined or separate influence of such factors during disease progression a precise characterization of aneurysm geometry evolution is needed. Multiple methods for geometrical parameterization of abdominal aortic aneurysms (AAAs) have been previously developed using isolated patient CT scan data but the focus has been mainly on the association of such geometrical parameters with the rupture risk and the efficacy of the parameterization is not fully investigated for a longitudinal study yet (multiple CT scans per patient at progressive intervals) [1]. For this study we have produced a series of 3D models for AAAs in longitudinal studies, developed an arterial centerline generation algorithm, and automated a geometric parameterization procedure for the arterial surfaces. It should be noted that the caliber of our collection of data is relatively rare as it is high resolution, features many patients, and on average has 4–5 images per patient.

Portable and Rapid In Vivo Imaging of Tissue Oxygenation Changes Induced by Skin Perfusion Pressure

2019 ◽  
Vol 42 ◽  
pp. 59-66 ◽  
Author(s):  
Audrey Huong ◽  
Xavier Ngu
Keyword(s):  
Vascular Reactivity ◽  
Imaging System ◽  
Statistical Significance ◽  
Systolic Pressure ◽  
Improve Patient Care ◽  
Healthcare Management ◽  
Oxygen Saturation Level ◽  
Healthy Humans ◽  
Time Required

Current imaging systems available in the study of tissue hemodynamics and vascular reactivity are typically complex and bulky, hence limiting their applications to laboratory use. The aim of this study is to present the dynamics of skin oxygen level with changes in the microcirculatory perfusion monitored using a developed field portable, handheld tri-wavelength imaging system. The skin oxygen saturation level (StO2) was measured in-vivo in a span of 12 minutes in the ventral forearm of seven healthy humans at rest, before and after supra-diastolic and supra-systolic pressure inflations. The findings of this work showed statistical significance in the difference between the mean StO2 values in baseline and that following ischemic episodes with ρ ≤ 0.03. The values returned to baseline, although of different magnitude for each individual, within 2 min (ρ =0.217) during reperfusion could suggest the time required for the resume of normal autoregulation mechanisms and vasomotion reactivities in the recruits. This study concluded that the developed imaging system could find potential application in self-healthcare management and may help to improve patient care in remote or rural locations.

Sign in / Sign up

Export Citation Format

Share Document