Messenger RNA transcripts coding for progesterone receptor A and B isoforms are expressed in human osteoblast cells

1998 ◽  
Vol 26 (1) ◽  
pp. 1-7 ◽  
Author(s):  
P. MacNamara ◽  
H. C. Loughrey
Keyword(s):  
Progesterone Receptor ◽  
Messenger Rna ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Rna Transcripts

3 Studies on Progesterone Receptor expression in Human Osteoblast Cells

1998 ◽  
Vol 26 (1) ◽  
pp. S1-S1
Author(s):  
Peter Mac Namara ◽  
Jeremy Skillington ◽  
Helen C. Loughrey
Keyword(s):  
Progesterone Receptor ◽  
Receptor Expression ◽  
Progesterone Receptor Expression ◽  
Human Osteoblast ◽  
Osteoblast Cells

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

scholarly journals Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Haiping Zhang ◽  
Ziliang Yu ◽  
Farui Sun ◽  
Jin Jin
Keyword(s):  
Protein Expression ◽  
Underlying Mechanism ◽  
R Package ◽  
Treated Group ◽  
Gene Expression Omnibus ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Gene And Protein Expression ◽  
Osteoblast Apoptosis ◽  
Induced Apoptosis

Abstract Background The purpose of the current study was to explore the role and underlying mechanism of cellular retinoic acid binding protein 2 (CRABP2) in dexamethasone (DEX)-induced apoptosis in human osteoblast cells. Methods GSE10311 was downloaded from the Gene Expression Omnibus (GEO) database to identify the differentially expressed genes (DEGs) by the limma/R package. Primary human osteoblast was isolated and treated with different concentration of DEX (0, 10-8, 10-7, 10-6, 10-5, and 10-4 mol/L), and cell viability and flow cytometry were used to detect cell proliferation and apoptosis. A CRABP2 overexpression plasmid (oe-CRABP2) was used to overexpress CRABP2, and western blotting was conducted to detect protein expression. Results We found that CRABP2 was downregulated in the DEX-treated group. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that DEGs were associated with PI3K/Akt signaling pathway. DEX downregulated CRABP2 gene and protein expression, inhibited viability, and induced human osteoblast apoptosis. Overexpression of CRABP2 reversed DEX-induced apoptosis in human osteoblast. Moreover, overexpression of CRABP2 delayed the progression of DEX-induced osteonecrosis of the femoral head (ONFH) animal model. Conclusion In conclusion, CRABP2 is effective at inhibiting DEX-induced human osteoblast apoptosis and delayed ONFH progression.

Methylene blue mediated photobiomodulation on human osteoblast cells

2017 ◽  
Vol 32 (8) ◽  
pp. 1847-1855 ◽  
Author(s):  
Gamze Bölükbaşı Ateş ◽  
Ayşe Ak ◽  
Bora Garipcan ◽  
Murat Gülsoy
Keyword(s):  
Methylene Blue ◽  
Human Osteoblast ◽  
Osteoblast Cells

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

Cellulose ◽  
2017 ◽  
Vol 24 (10) ◽  
pp. 4235-4252 ◽  
Author(s):  
Selestina Gorgieva ◽  
Vera Vivod ◽  
Uroš Maver ◽  
Lidija Gradišnik ◽  
Jurij Dolenšek ◽  
...  
Keyword(s):  
Cellulose Nanocrystals ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Modified Cellulose

scholarly journals Messenger RNA transcripts of the meiotic regulator BOULE in the testis of azoospermic men and their application in predicting the success of sperm retrieval

2005 ◽  
Vol 20 (3) ◽  
pp. 782-788 ◽  
Author(s):  
Yung Ming Lin ◽  
Pao Lin Kuo ◽  
Ying Hung Lin ◽  
Yen Ni Teng ◽  
Johnny Shinn Nan Lin
Keyword(s):  
Messenger Rna ◽  
Sperm Retrieval ◽  
Rna Transcripts
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