123 Two-dimensional electrophoresis of human urine and cerebrospinal fluid following protein concentration by dye precipitation

1997 ◽  
Vol 25 (4) ◽  
pp. S657-S657 ◽  
Author(s):  
THOMAS MARSHALL ◽  
KATHERINE M. WILLIAMS
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Urine ◽  
Protein Concentration ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Enhancement techniques for detecting trace and fluid-specific components in two-dimensional electrophoresis patterns.

1982 ◽  
Vol 28 (4) ◽  
pp. 759-765 ◽  
Author(s):  
G B Dermer ◽  
L M Silverman ◽  
J F Chapman
Keyword(s):  
Cerebrospinal Fluid ◽  
Serum Proteins ◽  
Prostatic Acid Phosphatase ◽  
Two Dimensional ◽  
Cibacron Blue ◽  
Two Dimensional Electrophoresis ◽  
Prostatic Fluid ◽  
Creatine Kinase B ◽  
Malignant Effusions ◽  
Dimensional Electrophoresis

Abstract Albumin and other serum-derived proteins were removed from several types of body fluids by affinity chromatography, to facilitate detection of trace or non-serum-derived proteins in two-dimensional electrophoresis patterns. Albumin was removed by the dye Cibacron Blue F3G-A coupled to Sepharose. Two-dimensional patterns of albumin-depleted serum lack the large albumin spot, and several families of spots become visible that ordinarily are partly or totally hidden by it. However, other proteins also bind to Cibacron Blue. Most serum proteins, including albumin, were effectively removed by anti-human serum antibodies coupled to Sepharose. Two-dimensional patterns of serum-depleted cerebrospinal fluid exhibit five clusters of probable nervous-system protein families not detected in serum. One additional family, probably antigenically related to transferrin, was removed by the affinity step. Two-dimensional patterns of serum-depleted prostatic fluid exhibit five major non-serum families, two of which may be creatine kinase B subunits and prostatic acid phosphatase. Two-dimensional patterns of serum-depleted malignant effusions exhibit one or more of three proteins that possibly are tumor products. Pattern matching suggests the presence of one non-serum-derived protein family common to cerebrospinal fluid, prostatic fluid, and malignant effusions. Prostatic fluid and malignant effusions have in common as many as three non-serum families of proteins.

Assessing cerebrospinal fluid rhinorrhea: A two-dimensional electrophoresis approach

2001 ◽  
Vol 22 (9) ◽  
pp. 1826-1833 ◽  
Author(s):  
Pierre R. Burkhard ◽  
Neftali Rodrigo ◽  
Daniel May ◽  
Roman Sztajzel ◽  
Jean-Charles Sanchez ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Two Dimensional ◽  
Cerebrospinal Fluid Rhinorrhea ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Development of a high-throughput method for preparing human urine for two-dimensional electrophoresis

PROTEOMICS ◽  
2005 ◽  
Vol 5 (9) ◽  
pp. 2315-2318 ◽  
Author(s):  
Graeme Smith ◽  
Derek Barratt ◽  
Rachel Rowlinson ◽  
Janice Nickson ◽  
Robert Tonge
Keyword(s):  
High Throughput ◽  
Human Urine ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
High Throughput Method ◽  
Dimensional Electrophoresis
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