Studies on the expression of the human erythrocyte glucose transporter (GLUT1) in the yeast Saccharomyces cerevisiae

1997 ◽  
Vol 25 (3) ◽  
pp. 463S-463S ◽  
Author(s):  
CLAIRE H. SIMONS ◽  
ADAM B. WEINGLASS ◽  
STEPHEN A. BALDWIN
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Human Erythrocyte ◽  
Glucose Transporter ◽  
Yeast Saccharomyces Cerevisiae ◽  
Glucose Transporter Glut1 ◽  
Transporter Glut1

scholarly journals Characterization of functional human erythrocyte-type glucose transporter (GLUT1) expressed in insect cells using a recombinant baculovirus

1992 ◽  
Vol 283 (3) ◽  
pp. 643-646 ◽  
Author(s):  
C K Yi ◽  
B M Charalambous ◽  
V C Emery ◽  
S A Baldwin
Keyword(s):  
Human Erythrocyte ◽  
Binding Sites ◽  
Glucose Transporter ◽  
Cytochalasin B ◽  
Insect Cells ◽  
Recombinant Baculovirus ◽  
Transport Inhibitor ◽  
Glucose Transporter Glut1 ◽  
Transporter Glut1

The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain greater than 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent Mr, probably as a result of glycosylation differences.

Polymorphisms of the glucose transporter (GLUT1) gene are associated with diabetic nephropathy

2001 ◽  
Vol 59 (3) ◽  
pp. 985-989 ◽  
Author(s):  
Andrea D. Hodgkinson ◽  
Beverley A. Millward ◽  
Andrew G. Demaine
Keyword(s):  
Diabetic Nephropathy ◽  
Glucose Transporter ◽  
Glucose Transporter Glut1 ◽  
Transporter Glut1 ◽  
Glut1 Gene

scholarly journals Paroxysmal exercise-induced dyskinesia and epilepsy is due to mutations in SLC2A1, encoding the glucose transporter GLUT1

Brain ◽  
2008 ◽  
Vol 131 (7) ◽  
pp. 1831-1844 ◽  
Author(s):  
A. Suls ◽  
P. Dedeken ◽  
K. Goffin ◽  
H. Van Esch ◽  
P. Dupont ◽  
...  
Keyword(s):  
Glucose Transporter ◽  
Exercise Induced ◽  
Glucose Transporter Glut1 ◽  
Transporter Glut1

scholarly journals Monosomy-3 Alters the Expression Profile of the Glucose Transporters GLUT1-3 in Uveal Melanoma

2020 ◽  
Vol 21 (24) ◽  
pp. 9345
Author(s):  
Tjorge Maaßen ◽  
Siranush Vardanyan ◽  
Anton Brosig ◽  
Hartmut Merz ◽  
Mahdy Ranjbar ◽  
...  
Keyword(s):  
Uveal Melanoma ◽  
Glucose Transporter ◽  
Glucose Transporters ◽  
Clinical Factors ◽  
Gene Encoding ◽  
Primary Tumors ◽  
High Affinity ◽  
Monosomy 3 ◽  
Glucose Transporter Glut1 ◽  
Transporter Glut1

Monosomy-3 in uveal melanoma (UM) cells increases the risk of fatal metastases. The gene encoding the low-affinity glucose transporter GLUT2 resides on chromosome 3q26.2. Here, we analyzed the expression of the glucose transporters GLUT1, GLUT2, and GLUT3 with regard to the histological and clinical factors by performing immunohistochemistry on the primary tumors of n = 33 UM patients. UMs with monosomy-3 exhibited a 57% lower immunoreactivity for GLUT2 and a 1.8×-fold higher ratio of GLUT1 to total GLUT1-3. The combined levels of GLUT1-3 proteins were reduced in the irradiated but not the non-irradiated tumors with monosomy-3. GLUT3 expression was stronger in the irradiated samples with disomy-3 versus monosomy-3, but the ratio of the GLUT3 isoform to total GLUT1-3 did not differ with regard to the monosomy-3 status in the irradiated or non-irradiated subgroups. Systemic metastases were associated with the presence of monosomy-3 in the primary and circulating tumor cells as well as a higher GLUT1 ratio. Upregulation of the high-affinity glucose transporter GLUT1 possibly as a compensation for the low-affinity isoform GLUT2 may be enhancing the basal glucose uptake in the UM cells with monosomy-3. Prevention of hyperglycemia might, therefore, be a valuable approach to delay the lethal UM metastases.

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