An investigation of the role of adenine phosphoribosyltransferase for excision repair following UV irradiation in Friend cells

1997 ◽  
Vol 25 (1) ◽  
pp. 149S-149S
Author(s):  
AILEEN A. NELSON ◽  
PATRICK G. MCKENNA ◽  
YVONNE A. BARNETT
Keyword(s):  
Uv Irradiation ◽  
Excision Repair ◽  
Adenine Phosphoribosyltransferase ◽  
Friend Cells

A mutation-promotive role of nucleotide excision repair in cell cycle-arrested cell populations following UV irradiation

DNA Repair ◽  
2010 ◽  
Vol 9 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Erich Heidenreich ◽  
Herfried Eisler ◽  
Theresia Lengheimer ◽  
Petra Dorninger ◽  
Ferdinand Steinboeck
Keyword(s):  
Cell Cycle ◽  
Nucleotide Excision Repair ◽  
Uv Irradiation ◽  
Excision Repair ◽  
Cell Populations ◽  
Nucleotide Excision

scholarly journals ANALYSIS OF THE ROLE OF RECOMBINATION AND REPAIR IN MUTAGENESIS OF ESCHERICHIA COLI BY UV IRRADIATION

Genetics ◽  
1977 ◽  
Vol 87 (1) ◽  
pp. 1-18
Author(s):  
Takesi Kato ◽  
Robert H Rothman ◽  
Alvin J Clark
Keyword(s):  
Escherichia Coli ◽  
Uv Irradiation ◽  
Excision Repair ◽  
Single Mutation ◽  
Uv Mutagenesis ◽  
Clear Plaque ◽  
Mutant Strains ◽  
Alternative Hypotheses ◽  
Mutagenic Process

ABSTRACT Multiple mutant strains have been tested for their mimicry of the UV-mutagenesis deficiency of a recA single mutant. Revertants to histidine prototrophy and clear plaque mutants of lambda were scored to determine capacity for UV-mutagenesis. Nearly normal capacity was shown by a uvr  +  recB  -  recF  - strain, which shows almost no recA-dependent recombination, by uvr  -  recB  +  recF  - strains, which show almost no recA-dependent repair and by a uvrA  -  recB  -  recF  - strain, which shows neither recA-dependent recombination nor repair. Since the uvr mutants can be assumed to show additionally no excision repair, these results may mean that UV-mutagenesis occurs during processes other than recombination and repair. Alternative hypotheses are discussed. The slight difference in mutagenic capacity was traced to the recF single mutation, which blocks the production of unmixed bursts of clear-plaque lambda mutants. Since this accounts for only about 10% of the mutations leading to clear-plaque mutants, it is suggested that there is more than one UV-mutagenic process.

scholarly journals The role of nucleotide excision repair in protecting embryonic stem cells from genotoxic effects of UV-induced DNA damage

1999 ◽  
Vol 27 (16) ◽  
pp. 3276-3282 ◽  
Author(s):  
P. P. H. Van Sloun ◽  
J. G. Jansen ◽  
G. Weeda ◽  
L. H. F. Mullenders ◽  
A. A. van Zeeland ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dna Damage ◽  
Embryonic Stem Cells ◽  
Nucleotide Excision Repair ◽  
Excision Repair ◽  
Embryonic Stem ◽  
Genotoxic Effects ◽  
Nucleotide Excision

scholarly journals Conversion of Methanol on Rutile TiO2 (110) and Tungsten Oxide Clusters: 2. The Role of Defects and Electron Transfer in Bifunctional Oxidic Photocatalysts

2021 ◽  
Author(s):  
Lars Mohrhusen ◽  
Jessica Kräuter ◽  
Katharina Al-Shamery
Keyword(s):  
Electron Transfer ◽  
Transition Metal ◽  
Metal Oxide ◽  
Tungsten Oxide ◽  
Organic Compounds ◽  
Uv Irradiation ◽  
Organic P ◽  
Rutile Tio2 ◽  
Metal Oxide Surfaces

The photochemical conversion of organic compounds on tailored transition metal oxide surfaces by (UV) irradiation has found wide applications ranging from the production of chemicals to the degradation of organic...

scholarly journals Damage sensor role of UV-DDB during base excision repair

2019 ◽  
Vol 26 (8) ◽  
pp. 695-703 ◽  
Author(s):  
Sunbok Jang ◽  
Namrata Kumar ◽  
Emily C. Beckwitt ◽  
Muwen Kong ◽  
Elise Fouquerel ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Base Excision

scholarly journals Structural studies on M. tuberculosis O6-methylguanine methyltransferase

2014 ◽  
Vol 70 (a1) ◽  
pp. C832-C832
Author(s):  
Menico Rizzi ◽  
Riccardo Miggiano ◽  
Samarpita Lahiri ◽  
Giuseppe Perugino ◽  
Maria Ciaramella ◽  
...  
Keyword(s):  
Excision Repair ◽  
Single Step ◽  
Double Stranded Dna ◽  
Nucleotide Excision ◽  
Enzymatic Systems ◽  
Methylguanine Methyltransferase ◽  
Mutagenic Effects ◽  
Wild Type Form

Mycobacterium tuberculosis (MTB) is an extremely well adapted human pathogen capable to survive for decades inside the hostile environment represented by the host's infected macrophages despite exposure to multiple potential DNA-damaging stresses. In order to maintain a remarkable low level of genetic diversity, MTB deploys different strategies of DNA repair, including multi-enzymatic systems, such as Nucleotide Excision Repair, and single-step repair. In particular, to counteract the mutagenic effects of DNA alkylation, MTB performs the direct alkylated-base reversal by sacrificing one molecule of a DNA-protein alkyltransferase, such as O6-methylguanine methyltransferase (OGT; orf: Rv1316c). We present here the biochemical and structural characterization of recombinant mycobacterial OGT (MtOGT) in its wild-type form along with its mutated variants mimicking the ones occurring in relevant clinical strains (i.e. MtOGT-T15S and MtOGT-R37L). Our studies reveal that MtOGT-R37L is severely impaired in its activity as consequence of its ten-fold lower affinity for modified double-stranded DNA (dsDNA) (1). Further investigations on a new structure-based panel of OGT versions, designed to explore different molecular environment at position 37, allowed us a better understanding of the functional role of the MtOGT Arg37-bearing loop during catalysis. Moreover, we solved the crystal structure of MtOGT in covalent complex with modified dsDNA that reveals an unprecedented MtOGT::DNA architecture, suggesting that the MtOGT monomer performing the catalysis needs assisting unreacted subunits during cooperative DNA binding. This work is supported by European Community FP7 program SYSTEMTB (Health-F4-2010-241587)

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