Antioxidant status, oxidative stress and DNA damage in the aetiology of Malnutrition Related Diabetes Mellitus

1997 ◽  
Vol 25 (1) ◽  
pp. 146S-146S ◽  
Author(s):  
MARGARET MCDONAGH ◽  
LIAQUAT ALI ◽  
AZAD KAHN ◽  
PETER R. FLATT ◽  
YVONNE A. BARNETT ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Dna Damage ◽  
Antioxidant Status

scholarly journals ASSESSMENT OF DNA STRAND BREAKS AND TOTAL ANTIOXIDANT STATUS IN TYPE 2 DIABETIC PATIENTS WITH AND WITHOUT COMPLICATIONS- A CASE-CONTROL STUDY

2017 ◽  
Vol 10 (4) ◽  
pp. 430
Author(s):  
K Nithya ◽  
Isabel W ◽  
Angeline T ◽  
Priscilla As ◽  
Asirvatham Aj
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Patients ◽  
Total Antioxidant

 Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in Type 2 diabetic patients (with and without complications) and controls.Materials and Methods: Blood samples were collected from 200 patients with type 2 diabetes mellitus (n=100 with complications and n=100 without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). Total antioxidant status was assessed by Ferric Reducing Ability of Plasma (FRAP) assay.Results: TAS was found to be significantly lower in type 2 diabetic patients (with and without complications) compared to controls (p< 0.001).  Similarly, patients with complications of type 2 diabetes mellitus had significantly lower TAS when compared to diabetic patients (p= 0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p< 0.001). Fasting glucose and glycosylated haemoglobin level (HbA1c) was found to be significantly higherin diabetic patients than controls (p< 0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA1c, TAS and DNA damage in patients with Type 2 diabetes mellitus.Conclusion: Alterations in TAS and the extent of DNA damage was observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.Key words: Hyperglycemia, Oxidative stress, DNA damage, Total antioxidant status, Type 2 diabetes mellitus, Vascular complication

scholarly journals Oxidative Stress, Antioxidant Status and DNA Damage in a Mercury Exposure Workers

2013 ◽  
Vol 4 (3) ◽  
pp. 80-88 ◽  
Author(s):  
Hasan F. Al-azzawie ◽  
Akram Umran ◽  
Nadhem H. Hyader
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Antioxidant Status ◽  
Mercury Exposure

scholarly journals Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus

2011 ◽  
Vol 34 (3) ◽  
pp. 163 ◽  
Author(s):  
Omur Tabak ◽  
Remise Gelisgen ◽  
Hayriye Erman ◽  
Fusun Erdenen ◽  
Cüneyt Muderrisoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Vascular Complications ◽  
Diabetic Group ◽  
Control Group ◽  
Diabetic Patients ◽  
Serum Samples ◽  
Type 2 Diabetic Patients

Purpose: The purpose of this study was to determine the effects of diabetic complications on oxidation of proteins, lipids, and DNA and to investigate the relationship between oxidative damage markers and clinical parameters. Methods: The study group consisted of 69 type 2 diabetic patients (20 patients without complication, 49 patients with complication) who attended internal medicine outpatient clinics of Istanbul Education and Research Hospital and 19 healthy control subjects. In serum samples of both diabetic patients and healthy subjects, 8-hydroxy-2’deoxyguanosine (8-OHdG), as a marker of oxidative DNA damage, Nε-(hexanoyl)lysine (HEL) and 15-F2t-iso-prostaglandin (15-F2t-IsoP). as products of lipooxidative damage, advanced oxidation protein products (AOPP), as markers of protein damage, and paraoxonase1 (PON1) as antioxidant were studied. Results: 15-F2t-IsoP (p < 0.005) and AOPP (p < 0.001) levels were significantly higher in diabetic group than control group while there were no significant differences in levels of 8-OHdG and HEL between the two groups. AOPP (p < 0.001) and 8-OHdG (p < 0.001) were significantly higher in diabetic group with complications compared to diabetic group without complications. Conclusions: Increased formation of free radicals and oxidative stress, under conditions of hyperglycaemia, is one of the probable causes for evolution of complications in diabetes mellitus. Our study supports the hypothesis that oxidant/antioxidant balance is disturbed in diabetic patients.

Association between genotoxic properties of inhalation anesthetics and oxidative stress biomarkers

2020 ◽  
Vol 36 (6) ◽  
pp. 454-466
Author(s):  
Masoud Neghab ◽  
Fatemeh Kargar-Shouroki ◽  
Hossein Mozdarani ◽  
Saeed Yousefinejad ◽  
Hamzeh Alipour ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Operating Room ◽  
Antioxidant Status ◽  
Venous Blood ◽  
Exposed Group ◽  
Oxidative Stress Biomarkers ◽  
Operating Room Personnel ◽  
Cross Sectional ◽  
Inhalation Anesthetics

Exposure to inhalation anesthetics (IAs) has been associated with DNA damage as reflected in the increased frequency of micronuclei (MN) and chromosomal aberrations (CAs). The present study was undertaken to ascertain whether there was any correlation between increased MN and CA and the extent of oxidative stress as well as the antioxidant status of a group of operating room personnel exposed to a mixture of IAs, including nitrous oxide, isoflurane, and sevoflurane. In this cross-sectional study, 60 operating room personnel (exposed group) in whom the frequencies of MN and CA had already been shown to be significantly higher than those of a referent group, as well as 60 unexposed nurses, were studied. Venous blood samples were taken from all participants, and malondialdehyde (MDA) levels as an index of oxidative stress (OS) and the activity of superoxide dismutase (SOD) and levels of total antioxidant capacity (TAC) as indices of antioxidant status were measured. The level of TAC (1.76 ± 0.59 mM vs. 2.13 ± 0.64 mM, p = 0.001) and the activity of SOD (11.22 ± 5.11 U/ml vs. 13.36 ± 4.12 U/ml, p = 0.01) were significantly lower, while the mean value of MDA was significantly higher (2.46 ± 0.66 µM vs. 2.19 ± 0.68 µM, p = 0.03) in the exposed group than in the nonexposed group. After adjusting for potential confounders, there were statistically significant associations between exposure to IAs, gender, SOD, and TAC with MN frequency and between exposure to IAs and SOD with numbers of CA. The findings of the present study indicated that exposure to IAs was associated with OS, and this, in turn, may be causally linked with DNA damage.

Oxidative stress and antioxidant status in elderly diabetes mellitus and glucose intolerance patients

2004 ◽  
Vol 39 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Teslime Atli ◽  
Kenan Keven ◽  
Aslihan Avci ◽  
Sim Kutlay ◽  
Nuran Turkcapar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Glucose Intolerance ◽  
Antioxidant Status

Antioxidant status and biomarkers of oxidative stress in cats with diabetes mellitus

2009 ◽  
Vol 93 (2) ◽  
pp. 145-145
Author(s):  
J. R. Smith ◽  
S. K. Cox ◽  
S. D. Lauten ◽  
R. C. Hill ◽  
J. W. Bartges ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Antioxidant Status ◽  
Biomarkers Of Oxidative Stress

Oxidative stress and antioxidant status in patients with late-onset gestational diabetes mellitus

2011 ◽  
Vol 50 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Cristina López-Tinoco ◽  
Mar Roca ◽  
Amor García-Valero ◽  
Mora Murri ◽  
Francisco J. Tinahones ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Antioxidant Status ◽  
Late Onset

scholarly journals Lipid Peroxidation and Antioxidant Status in Male and Female Patients with Type 1 and Type 2 Diabetes Mellitus

Med Phoenix ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 10-14
Author(s):  
Nirjala Laxmi Madhikarmi ◽  
Prem Prakash Singh ◽  
Tarannum Khatun
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Activity ◽  
Male And Female ◽  
Total Antioxidant

Background: Free radicals are reactive oxygen species which cause lipid peroxidation precipitating many metabolic diseases including Diabetes Mellitus. However, these free radicals are quenched by substances known as antioxidants like vitamin C, vitamin E and several other compounds. Lipid peroxidation and antioxidant status were investigated in patients with Type 1 and Type 2 Diabetes mellitus- Pokhara, Nepal.Methods: The extent of lipid peroxidation was assessed by thiobarbituric acid reactive substances and the antioxidant parameter estimations were total antioxidant activity, Vitamin C and Vitamin E assessed in Type 1 and 2 diabetes mellitus patients along with matched healthy counterparts.Results: The lipid peroxidation was increased in male Type 1 and 2 diabetic patients whereas female group showed decreased level as compared to its healthy counterparts. Similarly, the total antioxidant activity was found to be decreased in the diabetic group. The lipid peroxidation parameter and antioxidant status were statistically significant at p< 0.05.Conclusion: Oxidative stress and antioxidant status varied in male and female patients suffering from diabetes either Type 1 or Type 2. Apart from gender basis of evaluating oxidative stress, variables based on diet, habitat, socioeconomic status, education, etc. can also be considered.MED Phoenix Volume (1), Issue (1) July 2016, page: 10-14

Intrauterine exposure to flavonoids modifies antioxidant status at adulthood and decreases oxidative stress-induced DNA damage

2013 ◽  
Vol 57 ◽  
pp. 154-161 ◽  
Author(s):  
Kimberly Vanhees ◽  
Frederik J. van Schooten ◽  
Sahar Barjesteh van Waalwijk van Doorn-Khosrovani ◽  
Stefan van Helden ◽  
Armelle Munnia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Antioxidant Status ◽  
Intrauterine Exposure
Sign in / Sign up

Export Citation Format

Share Document