Teratogenic potential of free-radicals and hexokinase isoenzymes in the postimplantational “diabetic” rat conceptus

1996 ◽  
Vol 24 (2) ◽  
pp. 235S-235S ◽  
Author(s):  
REKHA SHARMA ◽  
DUMITRU CONSTANTIN-TEODOSIU ◽  
BRUCE MIDDLETON ◽  
MARTIN WILLIS ◽  
MARGARET K PRATTEN
Keyword(s):  
Free Radicals ◽  
Diabetic Rat ◽  
Teratogenic Potential ◽  
Hexokinase Isoenzymes

Regulation of spontaneous EDRF release in diabetic rat aorta by oxygen free radicals

1992 ◽  
Vol 263 (1) ◽  
pp. H257-H265 ◽  
Author(s):  
P. Langenstroer ◽  
G. M. Pieper
Keyword(s):  
Free Radicals ◽  
Oxygen Free Radicals ◽  
Muscle Tone ◽  
Aortic Ring ◽  
Rat Aorta ◽  
Diabetic Rat ◽  
Relaxing Factor ◽  
Oxygen Derived Free Radicals ◽  
Endothelium Derived Relaxing Factor ◽  
Vascular Smooth Muscle Tone

The interaction of endothelium-derived relaxing factor (EDRF) and oxygen-derived free radicals may potentially play an important role in the pathophysiology of complications associated with diabetes. In the present study, we investigated spontaneous EDRF release in diabetic rat aorta that is unmasked by the addition of superoxide dismutase (SOD). SOD produced a significantly greater relaxation in diabetic aorta compared with control aorta using both aortic ring and bioassay preparations. This relaxation was unaltered by pretreatment with catalase or indomethacin. Removal of the endothelium or pretreatment with either NG-monomethyl-L-arginine or methylene blue eliminated SOD-induced relaxation in both control and diabetic rings. Measurement of antioxidant enzymes revealed an elevation in catalase in diabetic aorta, with no difference in the SOD or glutathione peroxidase activity. The increase in catalase activity suggests increased exposure of diabetic aorta to hydrogen peroxide. Pretreatment of rings with the catalase inhibitor, 3-amino-1,2,4-triazole, attenuated the SOD-induced relaxation in diabetic aortic rings but had no effect in control aortic rings. In summary, our observations suggest that the diabetic rat aorta releases more spontaneous EDRF than control aorta; however, the activity of EDRF on vascular smooth muscle tone is masked by increased destruction by oxygen-derived free radicals.

Oxygen free radicals abolish endothelium-dependent relaxation in diabetic rat aorta

1988 ◽  
Vol 255 (4) ◽  
pp. H825-H833 ◽  
Author(s):  
G. M. Pieper ◽  
G. J. Gross
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Nordihydroguaiaretic Acid ◽  
Rat Aorta ◽  
Diabetic Rat ◽  
Lipoxygenase Inhibitor ◽  
Specificity And Sensitivity ◽  
Endothelium Dependent Relaxation ◽  
Generating System ◽  
Selective Impairment

The present experiments were conducted to determine whether endothelium-dependent relaxation is impaired in chronic (10-12 wk), streptozotocin-induced diabetic rat aortas and to determine the specificity and sensitivity of diabetic vasculature to oxygen-derived free radicals. Endothelium-dependent relaxation by acetylcholine and ADP was severely impaired in diabetic rat aorta, whereas endothelium-independent relaxation by nitroglycerin or papaverine was not impaired. Exposure to a free radical-generating system of xanthine plus xanthine oxidase caused a marked and prolonged relaxation in diabetic but not control vessels. Relaxation could not be prevented by the cyclooxygenase inhibitor indomethacin or the lipoxygenase inhibitor nordihydroguaiaretic acid but was attenuated or blocked by catalase. After free radical exposure, aortic rings were washed, reequilibrated, and contracted with a submaximal concentration of norepinephrine. In free radical-exposed vessels, endothelium-dependent relaxation by acetylcholine was reduced by 50% in nondiabetic vessels and abolished in diabetic vessels. Nevertheless, diabetic vessels could still be fully relaxed by nitroglycerin or papaverine. These results suggest selective impairment of endothelium-dependent relaxation in chronic diabetic rat aortas with particular sensitivity to free radical-induced damage.

scholarly journals Effect of Kembang Bulan Leaf (Tithonia diversifolia) Ethanolic Extract to SGOT and SGPT Levels in Diabetic Rats (Rattus norvegicus)

2021 ◽  
Vol 2 (1) ◽  
pp. 29-36
Author(s):  
Achmad Firdaus Pamudya Wardhana ◽  
Arifa Mustika ◽  
Citrawati Dyah Kenconowungu
Keyword(s):  
Free Radicals ◽  
Cell Damage ◽  
The Body ◽  
Diabetic Rat ◽  
Control Group ◽  
P Value ◽  
Tithonia Diversifolia ◽  
Control Group Design ◽  
Anova Test ◽  
Significant Difference

Introduction: Kembang Bulan (Tithonia diversifolia) is one of Indonesian traditional medicine that contains saponins, flavonoids, and, tannins and is predicted has a lowering effect on blood-glucose. Diabetes mellitus (DM) is a disease caused by insufficient insulin secretory by the pancreas or ineffective usage of insulin that produced by the body thus cause a hyperglycemia condition. Hyperglycemia can cause an increase in Reactive Oxygen Species which can cause free radicals. Free radicals can cause damage to liver cells. Method: This was an experiment with posttest control group design. Samples were 40 rats, divided into five different groups (P1, P2, P3, P4 and P5). Each group were induced by multiple low doses STZ. P1 (give the extract of Tithonia diversifolia at a dose 50 mg/100 gr BB), P2 (give the extract of Tithonia diversifolia at a dose 150 mg/100 gr BB), and P3 (give the extract of Tithonia diversifolia at a dose 450 mg/100 gr BB), P4 was treated with metformin, P5 was not treated. After STZ induction and give the extract to diabetic rat model, the resulting liver cell damage will be measured using the SGOT and SGPT measurement methods. Result: On the average results of SGOT levels in the five treatments, using the ANOVA test p value was 0.877 (p> 0.05) which means there were no significant differences. Furthermore, on the average results of SGPT levels in all five treatments, using the ANOVA test p value was 0.822 (p> 0.05) which means there were no significant differences. But from all results obtained P4 (given metformin) with the results closest to normal levels (SGOT: 197.5 ± 37.25; SGPT: 90.33 ± 29.36). From the treatment given Tithonia diversifolia extract obtained P1 (with a dose of 50 mg / 200gr BB) with the results closest to normal levels compared to P2 and P3. Conclusion: The Extract of kembang Bulan Leaf (Tithonia diversifolia) obtained less significant results compared to the untreated group. However, there is no significant difference. And giving ethanol extract of the leaves of kembang Bulan (Tithonia diversifolia)  at a dose of 50 mg / 100gr BB is the most effective way to reduce levels of SGOT and SGPT.

Troglitazone prevents falty changes of the liver in obese diabetic rat

2001 ◽  
Vol 120 (5) ◽  
pp. A207-A208
Author(s):  
D JIA ◽  
T AKIYAMA ◽  
K FUKUMITSU ◽  
M YAMAMOTO ◽  
S ABE ◽  
...  
Keyword(s):  
Diabetic Rat

Effects of empagliflozin on oxidative stress and endothelial dysfunction in STZ-induced Type 1 diabetic rat

2014 ◽  
Vol 9 (S 01) ◽  
Author(s):  
M Oelze ◽  
S Kröller-Schön ◽  
M Mader ◽  
E Zinßius ◽  
P Stamm ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Diabetic Rat

STRESS PADA LANSIA MENJADI FAKTOR PENYEBAB DAN AKIBAT TERJADINYA PENYAKIT

2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Nur Hidaayah
Keyword(s):  
Drinking Water ◽  
Free Radicals ◽  
The Elderly ◽  
The State ◽  
Young Age ◽  
Fresh Fruit ◽  
Stress Conditions ◽  
Harmful Substances ◽  
Available Resources

Stress conditions in the elderly means an imbalance condition of biological, psychological, and social are closely related to the response to the threats and dangers faced by the elderly. Pressure or interference that is not fun is usually created when the elderly see a mismatch between the state and the 3 systems available resources. Maintenance actions that need to be done there are 2 types, namely : prevention of exposure to a stressor (precipitation factor) and serious treatment of the imbalance condition/ illness (precipitation factor). Prevention includes: sports, hobbies, friendship, avoid eating foods high in free radicals and harmful substances, sex and setting arrangements adequate rest. Habits of the above if done at a young age to avoid exposure to stress in the elderly. Treatment of the imbalance condition / illness, include : drinking water, meditation, eating fresh fruit, and adequate rest.

Sign in / Sign up

Export Citation Format

Share Document