Stimulation of signal transduction pathways by MCP-1 in human monocytes and THP-1 cells

1996 ◽  
Vol 24 (1) ◽  
pp. 69S-69S ◽  
Author(s):  
Sarah J. Turner ◽  
Stephen G. Ward ◽  
Murray McKinnon ◽  
John Westwick
Keyword(s):  
Signal Transduction ◽  
Signal Transduction Pathways ◽  
Human Monocytes ◽  
Stimulation Of

scholarly journals Bovine glycomacropeptide induces cytokine production in human monocytes through the stimulation of the MAPK and the NF-κB signal transduction pathways

2009 ◽  
Vol 157 (7) ◽  
pp. 1232-1240 ◽  
Author(s):  
Pilar Requena ◽  
Abdelali Daddaoua ◽  
Emilia Guadix ◽  
Antonio Zarzuelo ◽  
María Dolores Suárez ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cytokine Production ◽  
Signal Transduction Pathways ◽  
Human Monocytes ◽  
Stimulation Of

scholarly journals Protein Kinase G Induces an Immune Response in Cows Exposed toMycobacterium aviumSubsp.paratuberculosis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Horacio Bach ◽  
Melissa Richard-Greenblatt ◽  
Eviatar Bach ◽  
Marcelo Chaffer ◽  
Wanika Lai ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Immune Response ◽  
Protein Kinase ◽  
Virulence Factors ◽  
Interferon Gamma ◽  
Host Cells ◽  
Protein Kinase G ◽  
Signal Transduction Pathways ◽  
Mycobacterial Species ◽  
Stimulation Of

To establish infection, pathogens secrete virulence factors, such as protein kinases and phosphatases, to modulate the signal transduction pathways used by host cells to initiate immune response. The protein MAP3893c is annotated in the genome sequence ofMycobacterium aviumsubspeciesparatuberculosis(MAP), the causative agent of Johne’s disease, as the serine/threonine protein kinase G (PknG). In this work, we report that PknG is a functional kinase that is secreted within macrophages at early stages of infection. The antigen is able to induce an immune response from cattle exposed to MAP in the form of interferon gamma production after stimulation of whole blood with PknG. These findings suggest that PknG may contribute to the pathogenesis of MAP by phosphorylating macrophage signalling and/or adaptor molecules as observed with other pathogenic mycobacterial species.

scholarly journals Characterization of Signal Transduction Pathways in Human Bone Marrow Endothelial Cells

Blood ◽  
1997 ◽  
Vol 90 (6) ◽  
pp. 2253-2259 ◽  
Author(s):  
Zhong-Ying Liu ◽  
Ramesh K. Ganju ◽  
Jian-Feng Wang ◽  
Karin Schweitzer ◽  
Babette Weksler ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Bone Marrow ◽  
Endothelial Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
T Antigen ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Signal Transduction Pathways ◽  
Stimulation Of

Human bone marrow endothelial cells immortalized with the T antigen of SV40 (TrHBMEC) have previously been characterized by us with regard to their properties that are similar to primary marrow endothelial cells and their utility as a model system. We now report that TrHBMEC express a recently discovered signal transduction molecule termed RAFTK (related adhesion focal tyrosine kinase), also called Pyk2 or CAK-β. RAFTK, the second member of the focal adhesion kinase (FAK) family, is known to be activated in response to calcium flux in neuronal cells and integrin stimulation in megakaryocytes and B cells. We have studied the effects of cytokines on RAFTK activation in TrHBMEC. Treatment of TrHBMEC with the vascular endothelial growth factor (VEGF ), as well as the VEGF-related protein (VRP), the recently identified ligand for the FLT-4 receptor, resulted in enhanced tyrosine phosphorylation of RAFTK. Similar changes in RAFTK phosphorylation were observed upon stimulation of TrHBMEC with basic fibroblast growth factor (bFGF ) or oncostatin M (OSM). Stimulation of these cells with growth factors also resulted in an increase in RAFTK activity and the c-Jun NH2 -terminal kinase (JNK). RAFTK coimmunoprecipitated with the cytoskeletal protein paxillin through its C-terminal proline-rich domain in TrHBMEC. These results suggest that, in marrow endothelium, activation of RAFTK by VEGF, VRP, OSM, and bFGF represents a new element in the signal transduction pathways used by these growth factors and likely acts to coordinate signaling from their surface receptors to the cytoskeleton, thereby modulating cell growth and function.

Signal transduction pathways in directed migration of human monocytes induced by human growth hormone in vitro

2001 ◽  
Vol 1 (7) ◽  
pp. 1351-1361 ◽  
Author(s):  
Christian M. Kähler ◽  
Andreas B. Pischel ◽  
Thomas Haller ◽  
Christian Meierhofer ◽  
Angela Djanani ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Signal Transduction Pathways ◽  
Human Monocytes ◽  
Directed Migration
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