Skeletal muscle development and the role of the myogenic regulatory factors

1996 ◽  
Vol 24 (2) ◽  
pp. 506-509 ◽  
Author(s):  
M. Buckingham
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Myogenic Regulatory Factors ◽  
Skeletal Muscle Development ◽  
Regulatory Factors

Myogenic regulatory factors transactivate the Tceal7 gene and modulate muscle differentiation

2010 ◽  
Vol 428 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Xiaozhong Shi ◽  
Daniel J. Garry
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Muscle Development ◽  
Cellular Proliferation ◽  
Elongation Factor ◽  
Muscle Differentiation ◽  
Myogenic Regulatory Factors ◽  
Mobility Shift ◽  
Regulatory Factors ◽  
E Box

Recurrent injuries eventually exhaust the capacity of skeletal muscle to fully restore or regenerate its cellular architecture. Therefore a comprehensive understanding of the muscle regeneration programme is needed to provide a platform for new therapies for devastating diseases such as Duchenne muscular dystrophy. To begin to decipher the molecular programme that directs muscle regeneration, we undertook an unbiased strategy using microarray analysis of cardiotoxin-injured skeletal muscle at defined time periods in the adult mouse. Using this strategy, we identified Tceal7 [transcription elongation factor A (SII)-like 7], which was dynamically regulated during muscle regeneration. Our studies revealed that Tceal7 was restricted to the skeletal muscle lineage during embryogenesis. Using transgenic technologies and transcriptional assays, we defined an upstream 0.7 kb fragment of the Tceal7 gene that directed the LacZ reporter to the developing skeletal muscle lineage. Analysis of the Tceal7 promoter revealed evolutionarily conserved E-box motifs within the 0.7 kb upstream fragment that were essential for promoter activity, as mutation of the E-box motifs resulted in the loss of reporter expression in the somites of transgenic embryos. Furthermore, we demonstrated that MRFs (myogenic regulatory factors) were Tceal7 upstream transactivators using transcriptional assays, EMSAs (electrophoretic mobility-shift assays), and ChIP (chromatin immunoprecipitation) assays. Overexpression of Tceal7 in C2C12 myoblasts decreased cellular proliferation and enhanced differentiation. Further studies revealed that p27 expression was up-regulated following Tceal7 overexpression. These studies support the hypothesis that MRFs transactivate Tceal7 gene expression and promote muscle differentiation during muscle development and regeneration.

scholarly journals The Role of Six1 in the Genesis of Muscle Cell and Skeletal Muscle Development

2014 ◽  
Vol 10 (9) ◽  
pp. 983-989 ◽  
Author(s):  
Wangjun Wu ◽  
Ruihua Huang ◽  
Qinghua Wu ◽  
Pinghua Li ◽  
Jie Chen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cell ◽  
Muscle Development ◽  
Skeletal Muscle Development

Dual role of delta-like 1 homolog (DLK1) in skeletal muscle development and adult muscle regeneration

Development ◽  
2013 ◽  
Vol 140 (18) ◽  
pp. 3743-3753 ◽  
Author(s):  
D. C. Andersen ◽  
J. Laborda ◽  
V. Baladron ◽  
M. Kassem ◽  
S. P. Sheikh ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Muscle Development ◽  
Dual Role ◽  
Skeletal Muscle Development ◽  
Adult Muscle

scholarly journals Transcriptome analysis reveals the long intergenic noncoding RNAs contributed to skeletal muscle differences between Yorkshire and Tibetan pig

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ziying Huang ◽  
Qianqian Li ◽  
Mengxun Li ◽  
Changchun Li
Keyword(s):  
Skeletal Muscle ◽  
Noncoding Rna ◽  
Muscle Development ◽  
Target Genes ◽  
Muscle Growth ◽  
Skeletal Muscle Development ◽  
Tibetan Pig ◽  
The Difference ◽  
Long Intergenic Noncoding Rna

AbstractThe difference between the skeletal muscle growth rates of Western and domestic breeds is remarkable, but the potential regulatory mechanism involved is still unclear. Numerous studies have pointed out that long intergenic noncoding RNA (lincRNA) plays a key role in skeletal muscle development. This study used published Yorkshire (LW) and Tibetan pig (TP) transcriptome data to explore the possible role of lincRNA in the difference in skeletal muscle development between the two breeds. 138 differentially expressed lincRNAs (DELs) were obtained between the two breeds, and their potential target genes (PTGs) were predicted. The results of GO and KEGG analysis revealed that PTGs are involved in multiple biological processes and pathways related to muscle development. The quantitative trait loci (QTLs) of DELs were predicted, and the results showed that most QTLs are related to muscle development. Finally, we constructed a co-expression network between muscle development related PTGs (MDRPTGs) and their corresponding DELs on the basis of their expression levels. The expression of DELs was significantly correlated with the corresponding MDRPTGs. Also, multiple MDRPTGs are involved in the key regulatory pathway of muscle fiber hypertrophy, which is the IGF-1-AKT-mTOR pathway. In summary, multiple lincRNAs that may cause differences in skeletal muscle development between the two breeds were identified, and their possible regulatory roles were explored. The findings of this study may provide a valuable reference for further research on the role of lincRNA in skeletal muscle development.

scholarly journals Differential physiological role of BIN1 isoforms in skeletal muscle development, function and regeneration

2018 ◽  
Author(s):  
Ivana Prokic ◽  
Belinda Cowling ◽  
Candice Kutchukian ◽  
Christine Kretz ◽  
Hichem Tasfaout ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Muscle Development ◽  
Physiological Role ◽  
Membrane Remodeling ◽  
Skeletal Muscle Development ◽  
Developmental Defects ◽  
Intracellular Organization ◽  
And Function

AbstractSkeletal muscle development and regeneration are tightly regulated processes. How the intracellular organization of muscle fibers is achieved during these steps is unclear. Here we focus on the cellular and physiological roles of amphiphysin 2 (BIN1), a membrane remodeling protein mutated in both congenital and adult centronuclear myopathies, that is ubiquitously expressed and has skeletal muscle-specific isoforms. We created and characterized constitutive, muscle-specific and inducible Bin1 homozygous and heterozygous knockout mice targeting either ubiquitous or muscle-specific isoforms. Constitutive Bin1-deficient mice died at birth from lack of feeding due to a skeletal muscle defect. T-tubules and other organelles were misplaced and altered, supporting a general early role of BIN1 on intracellular organization in addition to membrane remodeling. Whereas restricted deletion of Bin1 in unchallenged adult muscles had no impact, the forced switch from the muscle-specific isoforms to the ubiquitous isoforms through deletion of the in-frame muscle–specific exon delayed muscle regeneration. Thus, BIN1 ubiquitous function is necessary for muscle development and function while its muscle-specific isoforms fine-tune muscle regeneration in adulthood, supporting that BIN1 centronuclear myopathy with congenital onset are due to developmental defects while later onset may be due to regeneration defects.

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