Comparison of the lipolysis of chylomicron remnants derived from corn oil or olive oil by hepatic lipase in vitro

1995 ◽  
Vol 23 (2) ◽  
pp. 284S-284S ◽  
Author(s):  
KATHLEEN M. BOTHAM ◽  
PETER A. MAYES ◽  
MICHAEL AVELLA ◽  
ALFREDO CANTAFORA ◽  
ELENA BRAVO
Keyword(s):  
Olive Oil ◽  
Corn Oil ◽  
Hepatic Lipase ◽  
Chylomicron Remnants

scholarly journals Chylomicron-remnant uptake by freshly isolated hepatocytes. Effect of heparin and of hepatic triacylglycerol lipase

1989 ◽  
Vol 258 (2) ◽  
pp. 587-594 ◽  
Author(s):  
F Sultan ◽  
D Lagrange ◽  
X Le Liepvre ◽  
S Griglio
Keyword(s):  
Cholesteryl Ester ◽  
Hepatic Lipase ◽  
Isolated Hepatocytes ◽  
Chylomicron Remnant ◽  
Triacylglycerol Lipase ◽  
Cell Pellet ◽  
Chylomicron Remnants ◽  
Dose Response Study ◽  
Cholesteryl Ester Accumulation

Chylomicron remnants labelled biologically with [3H]cholesterol were efficiently taken up by freshly isolated hepatocytes during a 3 h incubation in Krebs bicarbonate medium. Their [3H]cholesteryl ester was hydrolysed (74% net hydrolysis), and 0.1 mM-chloroquine could partially inhibit this hydrolysis, provided that hepatocytes were first preincubated for 2 h 30 min at 37 degrees C. This hydrolysis was also measured in preincubated cells with remnants double-labelled (3H and 14C) on their free cholesterol moiety; [3H]cholesterol arising from [3H]cholesteryl ester hydrolysis was recovered in the free [3H]cholesterol pool. A dose-response study showed saturation of remnant uptake at 180 micrograms of remnant protein/10(7) cells. Heparin (10 units/ml) increased remnant uptake by 63% (P less than 0.01), [3H]cholesteryl ester accumulation in the cell pellet by 110% (P less than 0.025) and hepatic lipase activity secreted in the medium by 2.4-fold (P less than 0.01) and by 3.3-fold (P less than 0.01) at the end of the preincubation and incubation periods respectively. Addition of 100 munits of semi-purified hepatic lipase preparation/flask stimulated remnant uptake by 44-69%, and [3H]cholesteryl ester accumulation in the presence of chloroquine by 2.1-fold (P less than 0.025). When hepatic lipase was incubated solely with the remnants, it decreased their triacylglycerol and phospholipid contents by 24% and 26% respectively. Thus freshly isolated hepatocytes may be used to study chylomicron-remnant uptake. Hepatic lipase, which seems to underly the stimulating effect of heparin, facilitates remnant uptake in vitro, and this could be mediated by at least one (or both) of its hydrolytic properties.

scholarly journals Study of the In Vitro Digestion of Olive Oil Enriched or Not with Antioxidant Phenolic Compounds. Relationships between Bioaccessibility of Main Components of Different Oils and Their Composition

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 543 ◽  
Author(s):  
Jon Alberdi-Cedeño ◽  
María L. Ibargoitia ◽  
María D. Guillén
Keyword(s):  
Phenolic Compounds ◽  
Olive Oil ◽  
Corn Oil ◽  
Solid Phase ◽  
In Vitro Digestion ◽  
Proton Nuclear Magnetic Resonance ◽  
Gas Chromatography Mass Spectrometry ◽  
In Vitro Bioaccessibility ◽  
Main Components

The changes provoked by in vitro digestion in the lipids of olive oil enriched or not with different phenolic compounds were studied by proton nuclear magnetic resonance (1H NMR) and solid phase microextraction followed by gas chromatography/mass spectrometry (SPME-GC/MS). These changes were compared with those provoked in the lipids of corn oil and of virgin flaxseed oil submitted to the same digestive conditions. Lipolysis and oxidation were the two reactions under consideration. The bioaccessibility of main and minor components of olive oil, of phenolic compounds added, and of compounds formed as consequence of the oxidation, if any, were matters of attention. Enrichment of olive oil with antioxidant phenolic compounds does not affect the extent of lipolysis, but reduces the oxidation degree to minimum values or avoids it almost entirely. The in vitro bioaccessibility of nutritional and bioactive compounds was greater in the olive oil digestate than in those of other oils, whereas that of compounds formed in oxidation was minimal, if any. Very close quantitative relationships were found between the composition of the oils in main components and their in vitro bioaccessibility. These relationships, some of which have predictive value, can help to design lipid diets for different nutritional purposes.

Effects of vegetable oils on biochemical and biophysical properties of membrane retinal pigment epithelium cells

2013 ◽  
Vol 91 (10) ◽  
pp. 812-817 ◽  
Author(s):  
Toihiri Said ◽  
Jennifer Tremblay-Mercier ◽  
Hicham Berrougui ◽  
Patrice Rat ◽  
Abdelouahed Khalil
Keyword(s):  
Olive Oil ◽  
Membrane Fluidity ◽  
Vegetable Oils ◽  
Corn Oil ◽  
Retinal Pigment ◽  
Omega 3 ◽  
Biophysical Properties ◽  
Rpe Cells ◽  
Camelina Oil

The aim of this study was to investigate the effect of vegetable oil enrichment of retinal pigment epithelial (RPE) cells on their biochemical and biophysical properties. For this, RPE cells were incubated with 4 different vegetables oils (olive oil, corn oil, argan oil, and camelina oil). The cytotoxicity of these vegetable oils was assessed in vivo on 8-week-old mice and in vitro by using the neutral red and YO-PRO-1 tests. Membrane fluidity was evaluated by fluorescence anisotropy using the fluorescent probe diphenylhexatriene, and membrane fatty acid composition was assessed by gas chromatography. None of the oils tested displayed cytotoxic effects. In vitro, omega-3 rich oils improved membrane fluidity by 47% compared with the control cells. The omega-3 PUFA content within membranes decreased by 38% to 55% when cells were incubated separately with olive oil, corn oil, or argan oil, and increased when cells were incubated with a mixture of those oils, or with camelina oil alone (50% and 103% increase, respectively). Our results show that the fatty acids in vegetable oil incorporate into retinal cells and increase the plasma membrane fluidity.

scholarly journals Olive Fruit and Leaf Wastes as Bioactive Ingredients for Cosmetics—A Preliminary Study

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 245
Author(s):  
María de la Luz Cádiz-Gurrea ◽  
Diana Pinto ◽  
Cristina Delerue-Matos ◽  
Francisca Rodrigues
Keyword(s):  
Olive Oil ◽  
High Performance ◽  
Value Added ◽  
Bioactive Compound ◽  
In Vitro Assays ◽  
Oil Consumption ◽  
Cosmetic Ingredients ◽  
Bioactive Ingredients ◽  
Olive Industry

Olea europaea cultivar, native in the Mediterranean basin, has expanded worldwide, mainly due to the olive oil industry. This expansion is attributed to the benefits of olive oil consumption, since this product is rich in nutritional and bioactive compounds. However, the olive industry generates high amounts of wastes, which could be related to polluting effects on soil and water. To minimize the environmental impact, different strategies of revalorization have been proposed. In this sense, the aim of this work was to develop high cosmetic value added oleuropein-enriched extracts (O20 and O30), a bioactive compound from olive byproducts, performing a comprehensive characterization using high performance liquid chromatography coupled to mass spectrometry and evaluate their bioactivity by in vitro assays. A total of 49 compounds were detected, with oleuropein and its derivatives widely found in O30 extract, whereas iridoids were mainly detected in O20 extract. Moreover, 10 compounds were detected for the first time in olive leaves. Both extracts demonstrated strong antioxidant and antiradical activities, although O30 showed higher values. In addition, radical oxygen and nitrogen species scavenging and enzyme inhibition values were higher in O30, with the exception of HOCl and hyaluronidase inhibition assays. Regarding cell viability, olive byproduct extracts did not lead to a decrease in keratinocytes viability until 100 µg/mL. All data reported by the present study reflect the potential of industrial byproducts as cosmetic ingredients.

scholarly journals Novel effects of diets enriched with corn oil or with an olive oil/sunflower oil mixture on vitamin K metabolism and vitamin K-dependent proteins in young men

2002 ◽  
Vol 43 (6) ◽  
pp. 878-884
Author(s):  
Leon J. Schurgers ◽  
Martin J. Shearer ◽  
Berry A.M. Soute ◽  
Ibrahim Elmadfa ◽  
Julia Harvey ◽  
...  
Keyword(s):  
Olive Oil ◽  
Vitamin K ◽  
Sunflower Oil ◽  
Corn Oil ◽  
Young Men ◽  
Oil Sunflower

scholarly journals Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver.

1994 ◽  
Vol 35 (4) ◽  
pp. 709-720
Author(s):  
S Shafi ◽  
S E Brady ◽  
A Bensadoun ◽  
R J Havel
Keyword(s):  
Rat Liver ◽  
Hepatic Lipase ◽  
Chylomicron Remnants
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