The effects of dietary lipid manipulation upon the production of reactive oxygen species by murine peritoneal macrophages

1995 ◽  
Vol 23 (2) ◽  
pp. 280S-280S ◽  
Author(s):  
Philip C. Calder
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Peritoneal Macrophages ◽  
Dietary Lipid ◽  
Oxygen Species ◽  
Murine Peritoneal Macrophages

Role of distinct subpopulations of peritoneal macrophages in the regulation of reactive oxygen species release

1999 ◽  
Vol 27 (7-8) ◽  
pp. 797-809 ◽  
Author(s):  
M.Laura Fernández ◽  
Hebe A Durán ◽  
Silvia E O’Connor ◽  
Rómulo L Cabrini ◽  
Beatriz L Molinari
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Peritoneal Macrophages ◽  
Oxygen Species ◽  
Reactive Oxygen Species Release

Dietary Glutamine Supplementation Partly Reverses Impaired Macrophage Function Resulting From Overload Training in Rats

2015 ◽  
Vol 25 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Weihua Xiao ◽  
Peijie Chen ◽  
Jingmei Dong ◽  
Ru Wang ◽  
Beibei Luo
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Serum Testosterone ◽  
Training Group ◽  
Peritoneal Macrophages ◽  
Glutamine Supplementation ◽  
Control Group ◽  
Oxygen Species ◽  
Macrophage Function ◽  
Overload Training

The aim of this study was to evaluate the effect of overload training on the function of peritoneal macrophages in rats, and to test the hypothesis that glutamine in vivo supplementation would partly reverse the eventual functional alterations induced by overload training in these cells. Forty male Wistar rats were randomly divided into 5 groups: control group (C), overload training group (E1), overload training and restore one week group (E2), glutamine-supplementation group (EG1), and glutamine-supplementation and restore 1-week group (EG2). All rats, except those placed on sedentary control were subjected to 11 weeks of overload training protocol. Blood hemoglobin, serum testosterone, and corticosterone of rats were measured. Moreover, the functions (chemotaxis, phagocytosis, cytokines synthesis, reactive oxygen species generation) of peritoneal macrophages were determined. Data showed that blood hemoglobin, serum testosterone, corticosterone and body weight in the overload training group decreased significantly as compared with the control group. Meanwhile, the chemotaxis capacity (decreased by 31%, p = .003), the phagocytosis capacity (decreased by 27%, p = .005), the reactive oxygen species (ROS) generation (decreased by 35%, p = .003) and the cytokines response capability of macrophages were inhibited by overload training. However, the hindering of phagocytosis and the cytokines response capability of macrophages induced by overload training could be ameliorated and reversed respectively, by dietary glutamine supplementation. These results suggest that overload training impairs the function of peritoneal macrophages, which is essential for the microbicidal actions of macrophages. This may represent a novel mechanism of immunodepression induced by overload training. Nonetheless, dietary glutamine supplementation could partly reverse the impaired macrophage function resulting from overload training.

A functional folate receptor is induced during macrophage activation and can be used to target drugs to activated macrophages

Blood ◽  
2009 ◽  
Vol 113 (2) ◽  
pp. 438-446 ◽  
Author(s):  
Wei Xia ◽  
Andrew R. Hilgenbrink ◽  
Eric L. Matteson ◽  
Michael B. Lockwood ◽  
Ji-Xin Cheng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Macrophage Activation ◽  
Folate Receptor ◽  
Reactive Oxygen ◽  
Peritoneal Macrophages ◽  
Drug Uptake ◽  
Oxygen Species ◽  
Activation Markers ◽  
Activated State ◽  
Factor Α

Abstract Previous work has demonstrated that a subset of macrophages expresses a folate receptor (FR) that can mediate internalization of folate-linked molecules, including imaging and therapeutic agents. To characterize this subset, macrophages were collected from peritoneal cavities of mice injected with saline, thioglycolate, zymosan, heat-killed or live bacteria, and cell-surface markers that coexpress with FR were identified. Virtually no F4/80+ peritoneal macrophages from saline-injected mice expressed FR, whereas numerous macrophages from mice injected with each inflammatory stimulus expressed FR. Examination of cell differentiation antigens that are up-regulated in FR+ macrophages revealed markers characteristic of an activated state (CD80, CD86, Ly-6C/G), whereas macrophages lacking these activation markers expressed few or no FR. FR+ macrophages also produced tumor necrosis factor-α (TNF-α) and reactive oxygen species, and production of reactive oxygen species correlated linearly with expression of FR. Synovial macrophages collected from arthritic patients were found to bind and internalize folate-linked dyes. Moreover, a folate-linked radioimaging agent was shown to image inflamed joints of rheumatoid arthritic patients. These results suggest that FR constitutes a marker for macrophage activation and that FR+ macrophages can be targeted with folate-linked drugs without promoting drug uptake by nonactivated macrophages. This trial was registered at www.clinicaltrials.gov as #NCT00588393.

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