Codon bias in Escherichia coli may modulate translation initiation

1995 ◽  
Vol 23 (1) ◽  
pp. 76S-76S ◽  
Author(s):  
RICHARD F. COLLINS ◽  
MARTIN ROBERTS ◽  
DAVID A. PHOENIX
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Codon Bias

Codon bias at the 3′-side of the initiation codon is correlated with translation initiation efficiency in Escherichia coli

Gene ◽  
2001 ◽  
Vol 263 (1-2) ◽  
pp. 273-284 ◽  
Author(s):  
C.Magnus Stenström ◽  
Haining Jin ◽  
Louise L Major ◽  
Warren P Tate ◽  
Leif A Isaksson
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Codon Bias ◽  
Initiation Codon

scholarly journals Mutually exclusive RNA secondary structures regulate translation initiation of DinQ in Escherichia coli

RNA ◽  
2016 ◽  
Vol 22 (11) ◽  
pp. 1739-1749 ◽  
Author(s):  
Knut I. Kristiansen ◽  
Ragnhild Weel-Sneve ◽  
James A. Booth ◽  
Magnar Bjørås
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Secondary Structures ◽  
Rna Secondary Structures

scholarly journals Leaderless mRNAs Bind 70S Ribosomes More Strongly than 30S Ribosomal Subunits in Escherichia coli

2002 ◽  
Vol 184 (23) ◽  
pp. 6730-6733 ◽  
Author(s):  
Sean M. O'Donnell ◽  
Gary R. Janssen
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Primer Extension ◽  
Ribosomal Subunits ◽  
Ribosome Binding ◽  
Initiation Factors ◽  
Translation Initiation Factors

ABSTRACT By primer extension inhibition assays, 70S ribosomes bound with higher affinity, or stability, than did 30S subunits to leaderless mRNAs containing AUG or GUG start codons. Addition of translation initiation factors affected ribosome binding to leaderless mRNAs. Our results suggest that translation of leaderless mRNAs might initiate through a pathway involving 70S ribosomes or 30S subunits lacking IF3.

scholarly journals POLARITY IN SEGMENTS OF THE ESCHERICHIA COLI trp OPERON WITH DELETED INTRAOPERONIC TRANSLATIONAL INITIATION SIGNALS

Genetics ◽  
1975 ◽  
Vol 80 (4) ◽  
pp. 651-666
Author(s):  
Yasunobu Kano ◽  
Fumio Imamoto
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Nonsense Mutation ◽  
Messenger Rna ◽  
Distal Part ◽  
High Efficiency ◽  
Translational Initiation ◽  
Transcriptional Termination ◽  
Trp Operon

ABSTRACT The effect of deletion of the operator-distal genes of the trp operon, including the trpE-trpD intercistronic punctuation point, on the degree of transcriptional polarity (in this case the effect of a nonsense mutation on the level of mRNA from the distal part of the very gene where the mutation is located) was investigated. Double mutants which contain a nonsense mutation and a deletion in trpE were constructed, and the degree of transcriptional polarity was estimated by the decrease in messenger RNA for the operator-distal trpE beyond the nonsense mutation, as well as by the production of truncated messenger RNA for the region of trpE proximal to the nonsense mutation. The content of mRNA of operator-distal trpE and the size of the mRNA of operator-proximal trpE of the double mutants show that transcriptional polarity is not relaxed as a function of distance of the nonsense mutation from the operator-distal end of the trpE segment (at which the subsequent high efficiency translational initiation signal has been deleted). These findings are consistent with the conclusion that the degree of polarity depends on the distance of the nonsense mutation fro mthe subsequent translation initiation signal, but not on its distance from the operator-distal end, including possible translational or transcriptional termination signals

scholarly journals Escherichia coli Ribosomal Protein S1 Unfolds Structured mRNAs Onto the Ribosome for Active Translation Initiation

PLoS Biology ◽  
2013 ◽  
Vol 11 (12) ◽  
pp. e1001731 ◽  
Author(s):  
Mélodie Duval ◽  
Alexey Korepanov ◽  
Olivier Fuchsbauer ◽  
Pierre Fechter ◽  
Andrea Haller ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Translation Initiation ◽  
Ribosomal Protein S1

scholarly journals The Cryo-EM Structure of a Translation Initiation Complex from Escherichia coli

Cell ◽  
2005 ◽  
Vol 121 (5) ◽  
pp. 703-712 ◽  
Author(s):  
Gregory S. Allen ◽  
Andrey Zavialov ◽  
Richard Gursky ◽  
Måns Ehrenberg ◽  
Joachim Frank
Keyword(s):  
Escherichia Coli ◽  
Translation Initiation ◽  
Initiation Complex

scholarly journals Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli

2019 ◽  
Vol 294 (48) ◽  
pp. 18046-18056 ◽  
Author(s):  
Erik M. Leith ◽  
William B. O'Dell ◽  
Na Ke ◽  
Colleen McClung ◽  
Mehmet Berkmen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Translation Initiation ◽  
Translation Initiation Region ◽  
Internal Translation
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