Prostaglandins which elevate cyclic AMP increase low density lipoprotein receptor mRNA and activity in human extra-hepatic cells

1995 ◽  
Vol 23 (1) ◽  
pp. 5S-5S ◽  
Author(s):  
HEATHER NIELD ◽  
BRUCE MIDDLETON
Keyword(s):  
Cyclic Amp ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Receptor Mrna ◽  
Hepatic Cells ◽  
Density Lipoprotein Receptor

Involvement of second messengers in regulation of the low-density lipoprotein receptor gene

1989 ◽  
Vol 9 (6) ◽  
pp. 2298-2302
Author(s):  
J H Auwerx ◽  
A Chait ◽  
G Wolfbauer ◽  
S S Deeb
Keyword(s):  
Cyclic Amp ◽  
Cell Line ◽  
Second Messengers ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cell Surface Receptor ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Density Lipoprotein Receptor

Transcription of the low-density lipoprotein receptor (LDL-R) gene in the human monocytic leukemic cell line THP-1 and in the human hepatocarcinoma cell line Hep-G2 is regulated by second messengers of the diacylglycerol-protein kinase C (DAG-PKC), inositol 1,4,5-triphosphate-Ca2+, and cyclic AMP pathways. Exogenous phospholipase C (which releases DAG and inositol 1,4,5-triphosphate), PKC activators (phorbol esters and DAG), Ca2+ ionophores, and a cyclic AMP analog all transiently induced accumulation of LDL-R mRNA. The effects of these three signal-transducing pathways were to a large extent additive. Furthermore, PKC stimulation effected an increase in LDL binding, which suggested that the increase in LDL-R mRNA resulted in an increase in functional cell surface receptor activity. These results suggest that uptake of cholesterol by these cells is under control of both intracellular cholesterol levels and external signals.

Low density lipoprotein receptor mRNA in rat liver is affected by resistant starch of beans

Lipids ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Michihiro Fukushima ◽  
Tetsu Ohashi ◽  
Michiyuki Kojima ◽  
Kiyoshi Ohba ◽  
Hideki Shimizu ◽  
...  
Keyword(s):  
Rat Liver ◽  
Resistant Starch ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Receptor Mrna ◽  
Density Lipoprotein Receptor

scholarly journals Involvement of second messengers in regulation of the low-density lipoprotein receptor gene.

1989 ◽  
Vol 9 (6) ◽  
pp. 2298-2302 ◽  
Author(s):  
J H Auwerx ◽  
A Chait ◽  
G Wolfbauer ◽  
S S Deeb
Keyword(s):  
Cyclic Amp ◽  
Cell Line ◽  
Second Messengers ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Cell Surface Receptor ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Low Density Lipoprotein Receptor ◽  
Density Lipoprotein Receptor

Transcription of the low-density lipoprotein receptor (LDL-R) gene in the human monocytic leukemic cell line THP-1 and in the human hepatocarcinoma cell line Hep-G2 is regulated by second messengers of the diacylglycerol-protein kinase C (DAG-PKC), inositol 1,4,5-triphosphate-Ca2+, and cyclic AMP pathways. Exogenous phospholipase C (which releases DAG and inositol 1,4,5-triphosphate), PKC activators (phorbol esters and DAG), Ca2+ ionophores, and a cyclic AMP analog all transiently induced accumulation of LDL-R mRNA. The effects of these three signal-transducing pathways were to a large extent additive. Furthermore, PKC stimulation effected an increase in LDL binding, which suggested that the increase in LDL-R mRNA resulted in an increase in functional cell surface receptor activity. These results suggest that uptake of cholesterol by these cells is under control of both intracellular cholesterol levels and external signals.

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