Storage effects on the ligand binding properties of bovine heart cytochrome c oxidase

1994 ◽  
Vol 22 (3) ◽  
pp. 286S-286S ◽  
Author(s):  
A. JOHN MOODY ◽  
M. RICHARDSON ◽  
PETER R. RICH
Keyword(s):  
Cytochrome C ◽  
Ligand Binding ◽  
Cytochrome C Oxidase ◽  
Binding Properties ◽  
Bovine Heart ◽  
Storage Effects

scholarly journals ‘CO2-ligated’ cytochrome c oxidase: characterization and comparison with the Cl- -ligated enzyme

1994 ◽  
Vol 302 (3) ◽  
pp. 821-826 ◽  
Author(s):  
A J Moody ◽  
M Richardson ◽  
J P Spencer ◽  
U Brandt ◽  
P R Rich
Keyword(s):  
Electron Transfer ◽  
Cytochrome C ◽  
Ligand Binding ◽  
Cytochrome C Oxidase ◽  
Absorption Spectra ◽  
Intramolecular Electron Transfer ◽  
Endogenous Ligand ◽  
Binding Properties ◽  
Visible Absorption ◽  
Bovine Heart

A form of fully oxidized bovine heart cytochrome c oxidase that is induced by CO2/HCO3- is described. The ligand-binding properties of this form are similar to those of Cl(-)-ligated oxidase [Moody, Cooper and Rich (1991) Biochim. Biophys. Acta 1059, 189-207]. Both bind cyanide at a rate (0.2 M-1.s-1 at pH 6.5) intermediate between the rate of binding to the fast and slow forms of the enzyme, and binding of formate to both is almost undetectable. They are also similar in showing poor reactivity with H2O2, or with CO in the presence of O2, which, with fast oxidase, induce the formation of the ‘ferryl’ and ‘peroxy’ states respectively. However, there is a clear difference in the near-u.v./visible absorption spectra of the two forms; that induced by CO2/HCO3- has a Soret maximum at 427 nm whereas Cl(-)-ligated oxidase has a Soret maximum similar to that of fast oxidase at about 424 nm. It appears that both CO2/HCO3- and Cl- are members of a class of ligands that lowers the reactivity of the binuclear centre but does not impede intramolecular electron transfer from haem a to the binuclear centre, unlike the putative endogenous ligand responsible for slow oxidase.

Ligand binding properties of bacterial oxidases in relation to cytochrome-c oxidase

1992 ◽  
Vol 20 (3) ◽  
pp. 240S-240S ◽  
Author(s):  
A. JOHN MOODY ◽  
ROBERT B. GENNIS ◽  
DAVID B. HICKS ◽  
W. JOHN INGLEDEW ◽  
TERRY ANN KRULWICH ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Ligand Binding ◽  
Cytochrome C Oxidase ◽  
Binding Properties

The isolation of bovine-heart cytochrome c oxidase subunits Dependence on phospholipid and cholate content

1979 ◽  
Vol 548 (2) ◽  
pp. 397-416 ◽  
Author(s):  
F.E.A.M. Verheul ◽  
J.C.P. Boonman ◽  
J.W. Draijer ◽  
A.O. Muijsers ◽  
D. Borden ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Bovine Heart
Sign in / Sign up

Export Citation Format

Share Document