Elucidation of the role of glutathione reductase using transgenic plants

1994 ◽  
Vol 22 (4) ◽  
pp. 931-936 ◽  
Author(s):  
Philip Mullineaux ◽  
Gary Creissen ◽  
Pamela Broadbent ◽  
Helen Reynolds ◽  
Baldeep Kular ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Glutathione Reductase

Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells

2013 ◽  
Vol 34 (24) ◽  
pp. 3305-3314 ◽  
Author(s):  
Chiao-Yuan Fan ◽  
Hsiu-Chuan Chou ◽  
Yi-Wen Lo ◽  
Yueh-Feng Wen ◽  
Yi-Chih Tsai ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Glutathione Reductase ◽  
Cancer Cells ◽  
Human Lung ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Human Lung Cancer Cells ◽  
Proteomic Study

Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition

2006 ◽  
Vol 33 (2) ◽  
pp. 153 ◽  
Author(s):  
Mohammad S. Hoque ◽  
Josette Masle ◽  
Michael K. Udvardi ◽  
Peter R. Ryan ◽  
Narayana M. Upadhyaya
Keyword(s):  
Transgenic Plants ◽  
Ammonium Assimilation ◽  
Ammonium Transporter ◽  
Ammonium Uptake ◽  
Vegetative Stage ◽  
Over Expression ◽  
Transgenic Lines ◽  
Ammonium Nutrition ◽  
Maize Ubiquitin Promoter

A transgenic approach was undertaken to investigate the role of a rice ammonium transporter (OsAMT1-1) in ammonium uptake and consequent ammonium assimilation under different nitrogen regimes. Transgenic lines overexpressing OsAMT1-1 were produced by Agrobacterium-mediated transformation of two rice cultivars, Taipei 309 and Jarrah, with an OsAMT1-1 cDNA gene construct driven by the maize ubiquitin promoter. Transcript levels of OsAMT1-1 in both Taipei 309 and Jarrah transgenic lines correlated positively with transgene copy number. Shoot and root biomass of some transgenic lines decreased during seedling and early vegetative stage compared to the wild type, especially when grown under high (2 mm) ammonium nutrition. Transgenic plants, particularly those of cv. Jarrah recovered in the mid-vegetative stage under high ammonium nutrition. Roots of the transgenic plants showed increased ammonium uptake and ammonium content. We conclude that the decreased biomass of the transgenic lines at early stages of growth might be caused by the accumulation of ammonium in the roots owing to the inability of ammonium assimilation to match the greater ammonium uptake.

scholarly journals PREVALENCE, ASSOCIATED FACTORS AND ROLE OF VITAMIN D AND GLUTATHIONE REDUCTASE OF PRE-ECLAMPSIA IN PREGNANT WOMEN

2020 ◽  
Vol 4 (5) ◽  
Author(s):  
Simerpreet Kukreja
Keyword(s):  
Fatty Acids ◽  
Vitamin D ◽  
Pregnant Women ◽  
Glutathione Reductase ◽  
Gestational Hypertension ◽  
Multiple Pregnancy ◽  
Vitamin D Supplementation ◽  
Research Centre ◽  
Biochemical Basis

Introduction: In maternal and neonatal cases, preeclampsia is a multi-organ, heterogeneous pregnancy condition associated with significant morbidity and mortality. Since preeclampsia is a progressive disease, in some cases, delivery is necessary to stop the progression to the benefit of the mother and foetus. However, the need for early delivery has adverse effects on significant neonatal outcomes that are not limited to the most premature babies. The results include oxidative stress in the disease and invoke the biochemical basis for antioxidant clinical trials to prevent and treat hypertension caused by pregnancy. In the management of preeclampsia, supplementation of antioxidants along with polyunsaturated fatty acids, particularly omega-3 fatty acids, may be useful. This describes vitamin Ds potential role in the pathogenesis of preeclampsia. However, the role of vitamin D supplementation and dosing is controversial in preventing preeclampsia. Method: The study was carried out from March 2019 to April 2020 at Shalinitai Meghe Hospital and Research Centre using institutional-based cross-sectional study design among women whose age was greater than or equal to eighteen. Data were collected using a standardised and pretested questionnaire from 150 participants by face-to - face interview technique. Using Chemiluminiscent Immunoassay (CLIA), vitamin D estimation was performed. The behaviour of Glutathione Reductase was calculated according to the Goldberg et al 1983 procedure. To classify the factors associated with the development of preeclampsia, logistic regression analysis was used. Result: With a mean age of 30.28, a total of 150 participants were enrolled in the study. Evaluation of vitamin D and glutathione levels The prevalence of preeclampsia among current pregnant women attending ANC at Shalinitai Meghe Hospital was 16 with a 95 % CI. The current preeclampsia was significantly correlated with predictive variables such as the age of the respondents, current multiple pregnancy, and history of diabetes mellitus. Conclusion: The findings of this study showed that preeclampsia was present in a large proportion of women. For both urban and rural residents, health seeking actions towards pregnant women should be promoted, offering an opportunity to detect preeclampsia as early as possible and preventing the coming complication of preeclampsia. The role of antioxidants is controversial in the prevention of preeclampsia. Vitamin D deficiency is associated with preeclampsia in a major way. To document the role of vitamin D supplementation in the prevention of preeclampsia, further studies are required.  Keywords: Gestational hypertension, Pre-eclampsia, Vitamin D, GSH

Evidence of a significant role of glutathione reductase in the sulfur assimilation pathway

2020 ◽  
Vol 102 (2) ◽  
pp. 246-261
Author(s):  
Anner Cohen ◽  
Yael Hacham ◽  
Yochai Welfe ◽  
Soliman Khatib ◽  
Jean‐Christophe Avice ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Significant Role ◽  
Sulfur Assimilation

The role of glutathione reductase in paraquat neurotoxicity

2012 ◽  
Vol 211 ◽  
pp. S165-S166
Author(s):  
Mirjana Djukic ◽  
Marina Jovanovic ◽  
Milica Ninkovic ◽  
Ivana Stevanovic
Keyword(s):  
Glutathione Reductase

Role of Glutathione Reductase in Plant Abiotic Stress

2011 ◽  
pp. 149-158 ◽  
Author(s):  
Peerzada Yasir Yousuf ◽  
Khalid Ul Rehman Hakeem ◽  
Ruby Chandna ◽  
Parvaiz Ahmad
Keyword(s):  
Abiotic Stress ◽  
Glutathione Reductase ◽  
Plant Abiotic Stress

Cloning and characterization of a thermostable glutathione reductase from a psychrophilic Arctic bacterium Sphingomonas sp.

2019 ◽  
Author(s):  
Hai VuThi ◽  
Sei-Heon Jang ◽  
ChangWoo Lee
Keyword(s):  
Thermal Stability ◽  
Glutathione Reductase ◽  
Redox Homeostasis ◽  
Residual Activity ◽  
Reducing Power ◽  
Composition Analysis ◽  
Psychrophilic Bacterium ◽  
Tertiary Structures

Abstract Glutathione reductase is an important oxidoreductase that helps maintain redox homeostasis by catalyzing the conversion of glutathione disulfide to glutathione using NADPH as a cofactor. In this study, we cloned and characterized a glutathione reductase (referred hereafter to as SpGR) from Sphingomonas sp. PAMC 26621, an Arctic bacterium. SpGR comprises 449 amino acids, and functions as a dimer. Surprisingly, SpGR exhibits characteristics of thermophilic enzymes, showing optimum activity at 60°C and thermal stability up to 70 °C with approximately 50% residual activity at 70 °C for 2 h. The amino acid composition analysis of SpGR showed a 1.9-fold higher Arg content (6%) and a 2.7-fold lower Lys/Arg ratio (0.75) compared to the Arg content (3.15%) and the Lys/Arg ratio (2.01) of known psychrophilic glutathione reductases. SpGR also exhibits its activity at 4°C, and circular dichroism and fluorescence spectroscopy results indicate that SpGR maintains its secondary and tertiary structures within the temperature range 4–70°C. Taken together, the results of this study indicate that despite its origin from a psychrophilic bacterium, SpGR has high thermal stability. Our study provides an insight into the role of glutathione reductase in maintaining the reducing power of an Arctic bacterium in a broad range of temperatures.

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