Detection of protein kinase C subspecies expression at the mRNA level using a reverse transcriptase-polymerase chain reaction approach

1993 ◽  
Vol 21 (4) ◽  
pp. 379S-379S
Author(s):  
Leigh M. Eastwood ◽  
Martin G. Rumsby ◽  
F. Arthur McMorris
Keyword(s):  
Polymerase Chain Reaction ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Reverse Transcriptase ◽  
Mrna Level ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Approach ◽  
Kinase C ◽  
Polymerase Chain

Differential Expression of Kinase Genes in Primary Hyperparathyroidism: Adenoma Versus Normal and Hyperplastic Parathyroid Tissue

2007 ◽  
Vol 131 (1) ◽  
pp. 126-130
Author(s):  
Pinhas P. Schachter ◽  
Suhail Ayesh ◽  
Imad Matouk ◽  
Tamar Schneider ◽  
Abraham Czerniak ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Protein Kinase ◽  
Reverse Transcriptase ◽  
Parathyroid Adenoma ◽  
Parathyroid Tissue ◽  
Cellular Growth ◽  
Mitogen Activated Protein Kinase ◽  
Calcium Handling ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Context.—Differentiation between adenoma and hyperplasia or even normal parathyroid tissue is difficult and based mainly on the surgeon's skill. Exploration of genes that express differentially in these various tissues using microarrays and other sophisticated research tools will enable identification and perhaps development of new methods of perioperative diagnosis. Objective.—To assemble a panel of kinase genes to differentiate parathyroid adenoma from normal and hyperplastic parathyroid tissue. Design.—RNA was extracted from adenoma, hyperplasia, and normal parathyroid tissue and hybridized to a microarray containing 359 human cDNAs of known kinase genes. Signals of exposure were scanned and quantified with software for digital image analysis. Semiquantitative reverse transcriptase polymerase chain reaction analysis of sample genes was performed, up-regulated or down-regulated, to validate the microarray results. Results.—The ratio values considered significant (<0.5 or >1.5) suggest that genes up-regulated in parathyroid adenoma are those responsible for blood vessel angiogenesis and genes belonging to the cyclin-dependent kinase inhibitor groups. Genes down-regulated in parathyroid adenoma are related to cellular growth and apoptosis—genes from the mitogen-activated protein kinase group and DNA-dependent protein kinase group. An interesting gene down-regulated in the parathyroid adenoma samples is related to the serine/threonine protein kinases that exert a key function in calcium handling. A panel of 5 genes was defined: p19, p21 and the gene for vascular endothelial growth factor from the up-regulated group, and the gene for protein kinase C and SGK from the down-regulated group. Reverse transcriptase polymerase chain reaction confirmed the microarray results for these genes. Conclusions.—The kinase genes panel presented can be used to differentiate parathyroid adenoma from normal and hyperplastic parathyroid tissue in particular when histopathology fails to provide a decisive diagnosis.

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