Ribonucleotide reductases: Radical enzymes with suicidal tendencies

1993 ◽  
Vol 21 (3) ◽  
pp. 727-730 ◽  
Author(s):  
S. Booker ◽  
J. Broderick ◽  
J. Stubbe
Keyword(s):  
Ribonucleotide Reductases ◽  
Radical Enzymes

scholarly journals Ribonucleotide reductases: radical enzymes with suicidal tendencies

1995 ◽  
Vol 2 (12) ◽  
pp. 793-801 ◽  
Author(s):  
JoAnne Stubbe ◽  
Wilfred A. van der Donk
Keyword(s):  
Ribonucleotide Reductases ◽  
Radical Enzymes

Synthesis of a C-8 Modified Adenosine Analogue as a Potential Mechanistic Probe for Ribonucleotide Reductases

1999 ◽  
Vol 18 (4-5) ◽  
pp. 611-612 ◽  
Author(s):  
G. C. Magnin ◽  
J. Dauvergne ◽  
A. Burger ◽  
J-F. Biellmann
Keyword(s):  
Ribonucleotide Reductases ◽  
Mechanistic Probe ◽  
Adenosine Analogue

Identification and isolation of the gene encoding the small subunit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mitotic viability

1987 ◽  
Vol 7 (8) ◽  
pp. 2783-2793
Author(s):  
S J Elledge ◽  
R W Davis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Dna Damage ◽  
Ribonucleotide Reductase ◽  
Small Subunit ◽  
Cross Reaction ◽  
Expression Library ◽  
Gene Encoding ◽  
Ribonucleotide Reductases ◽  
Colony Color

Ribonucleotide reductase catalyzes the first step in the pathway for the production of deoxyribonucleotides needed for DNA synthesis. The gene encoding the small subunit of ribonucleotide reductase was isolated from a Saccharomyces cerevisiae genomic DNA expression library in lambda gt11 by a fortuitous cross-reaction with anti-RecA antibodies. The cross-reaction was due to an identity between the last four amino acids of each protein. The gene has been named RNR2 and is centromere linked on chromosome X. The nucleotide sequence was determined, and the deduced amino acid sequence, 399 amino acids, shows extensive homology with other eucaryotic ribonucleotide reductases. Transplason mutagenesis was used to disrupt the RNR2 gene. A novel assay using colony color sectoring was developed to demonstrate visually that RNR2 is essential for mitotic viability. RNR2 encodes a 1.5-kilobase mRNA whose levels increase 18-fold after treatment with the DNA-damaging agent 4-nitroquinoline 1-oxide. CDC8 was also found to be inducible by DNA damage, but POL1 and URA3 were not inducible by 4-nitroquinoline 1-oxide. The expression of these genes defines a new mode of regulation for enzymes involved in DNA biosynthesis and sharpens our picture of the events leading to DNA repair in eucaryotic cells.

De Novo Proteins as Models of Radical Enzymes†

Biochemistry ◽  
1999 ◽  
Vol 38 (29) ◽  
pp. 9495-9507 ◽  
Author(s):  
Cecilia Tommos ◽  
Jack J. Skalicky ◽  
Denis L. Pilloud ◽  
A. Joshua Wand ◽  
P. Leslie Dutton
Keyword(s):  
De Novo ◽  
De Novo Proteins ◽  
Radical Enzymes
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