The generation of free acid SBFI within the matrix of isolated rat heart mitochondria and its use to estimate matrix [Na+]

1992 ◽  
Vol 20 (1) ◽  
pp. 14S-14S
Author(s):  
RACHAEL L. DANIEL ◽  
NICOLA J. OSBALDESTON ◽  
JAMES G. McCORMACK
Keyword(s):  
Rat Heart ◽  
Free Acid ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
The Matrix ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

scholarly journals Measurement of matrix free Mg2+ concentration in rat heart mitochondria by using entrapped fluorescent probes

1990 ◽  
Vol 271 (3) ◽  
pp. 627-634 ◽  
Author(s):  
G A Rutter ◽  
N J Osbaldeston ◽  
J G McCormack ◽  
R M Denton
Keyword(s):  
Rat Heart ◽  
Free Acid ◽  
Magnesium Content ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Fura 2 ◽  
Kd Values ◽  
Carbonyl Cyanide ◽  
The Matrix ◽  
Rat Heart Mitochondria

1. The concentration of free Mg2+ ([Mg2+]m) within the matrix of isolated rat heart mitochondria was measured after loading of the mitochondria with the fluorescent Mg2+ indicators mag-indo-1 and mag-fura-2. No detectable change in total mitochondrial magnesium content occurred during loading with the indicators. Apparent Kd values for Mg2+ of 3.7 mM and 2.3 mM were obtained for mag-indo-1 and mag-fura-2 respectively within mitochondria permeabilized to bivalent cations with ionomycin and the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone. These values are 2.7- and 1.8-fold greater respectively than those obtained for the free acid forms of the dyes in incubation medium. 2. Based on the above Kd values, mitochondrial matrix Mg2+ concentrations were found to lie in the range 0.8-1.5 mM in the absence, or immediately after the addition, of a respiratory substrate. 3. Incubation of mitochondria in the presence of respiratory substrate, but in the absence of external Mg2+, led to a time-dependent decline in [Mg2+]m to about half the initial values after 5 min. This was accompanied by a fall in the total mitochondrial magnesium content from 12.7 to 7.0 nmol/mg of protein. 4. ADP (0.5 mM), ATP (0.5 mM) or 10 mM-NaCl had no significant effect on the fall in [Mg2+], whereas 1 microM-nigericin blocked, and 0.3 microM-valinomycin accelerated, the fall. 5. External Mg2+ concentrations above 1 mM progressively inhibited and reversed the decline in free and total mitochondrial Mg2+.

Effects of pinacidil and calcium on isolated rat heart mitochondria

2012 ◽  
Vol 443 (1) ◽  
pp. 113-117 ◽  
Author(s):  
S. M. Korotkov ◽  
L. V. Emel’yanova ◽  
I. V. Brailovskaya ◽  
V. P. Nesterov
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Uncoupling and Antioxidant Effects of Ursolic Acid in Isolated Rat Heart Mitochondria

2011 ◽  
Vol 74 (7) ◽  
pp. 1640-1644 ◽  
Author(s):  
Julius Liobikas ◽  
Daiva Majiene ◽  
Sonata Trumbeckaite ◽  
Lolita Kursvietiene ◽  
Ruta Masteikova ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Heart Mitochondria ◽  
Rat Heart Mitochondria ◽  
Antioxidant Effects ◽  
Isolated Rat

scholarly journals Celecoxib Decreases Mitochondrial Complex IV Activity and Induces Oxidative Stress in Isolated Rat Heart Mitochondria: An Analysis for its Cardiotoxic Adverse Effect

2020 ◽  
Author(s):  
Saman Atashbar ◽  
Elham Mohammad Khanlou ◽  
Saleh Khezri ◽  
Peyman Kurdpour ◽  
Ahmad Salimi
Keyword(s):  
Lipid Peroxidation ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Mitochondrial Swelling ◽  
Heart Mitochondria ◽  
Isolated Mitochondria ◽  
Biochemical Assays ◽  
Ros Formation ◽  
Rat Heart Mitochondria ◽  
Isolated Rat

Abstract Background In spite of the cardiotoxic effect of selective cyclooxygenase-2 inhibitors, they are most widely used as anti-inflammatory and analgesic drugs. Today, valdecoxib and rofecoxib have been withdrawn on the market but celecoxib remains. In this study, we focused on an analysis of celecoxib toxic effects on isolated mitochondrial. Methods isolated rat heart mitochondria were obtained using differential centrifugation. Using flowcytometry and biochemical assays we searched succinate dehydrogenases (SDH), mitochondrial membrane potential (MMP), reactive oxygen species (ROS) formation, mitochondrial swelling, lipid peroxidation and mitochondrial complexes activity in rat heart isolated mitochondria. Results In here our results indicated a significant decrease in activity of complexes IV after exposure with celecoxib (16 µg/ml). This decrease in activity of complexes IV is paralleled by the MMP collapse, ROS formation, mitochondrial swelling and lipid peroxidation. Conclusion For the first time, this introductory study has showed a significant decrease in activity of complexes IV and mitochondrial dysfunction after exposure with celecoxib in rat heart isolated mitochondria.

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