The determination of γ-glutamyl transferase (EC 2.3.2.2) with oxidized glutathione, glutamate dehydrogenase, NAD and tetrazolium salts. A method appropriate to kinetic measurements and enzymic staining after electrophoresis

1991 ◽  
Vol 19 (1) ◽  
pp. 51S-51S
Author(s):  
PETER C. NICHOLAS ◽  
DANIEL L. THOMAS
Keyword(s):  
Glutamate Dehydrogenase ◽  
Oxidized Glutathione ◽  
Kinetic Measurements ◽  
Tetrazolium Salts ◽  
Glutamyl Transferase

Formation of sodium disulfite in a solid-gas system

1991 ◽  
Vol 56 (8) ◽  
pp. 1575-1579 ◽  
Author(s):  
Jiří Vobiš ◽  
Karel Mocek ◽  
Emerich Erdös
Keyword(s):  
Sodium Sulfite ◽  
Heterogeneous Reaction ◽  
Optimum Conditions ◽  
Kinetic Measurements ◽  
Dissociation Pressure ◽  
Partial Pressures ◽  
Gas System ◽  
Gaseous Sulfur ◽  
Equilibrium Dissociation

The formation of sodium disulfite by the heterogeneous reaction of solid active sodium sulfite with gaseous sulfur dioxide in the presence of water vapour was investigated over the temperature range of 293 to 393 K at SO2. H2O and O2 partial pressures of 1.2-7.4, 1.2-6.4 and 0-11.3 kPa, respectively. The effect of the reaction time was also examined. Kinetic measurements were supplemented with the determination of the equilibrium dissociation pressure of SO2 in contact with sodium sulfite at 373.15 K. The major aim of the work was to establish the optimum conditions for attaining the maximum degree of conversion of the solid reactant to sodium disulfite. The conditions for the formation of virtually pure sodium disulfite were found.

scholarly journals Alanine Aminopeptidase, γ-Glutamyl Transferase and β2-microglobulin as Diagnostic Markers in Patients with Rheumatoid Arthritis

2008 ◽  
Vol 27 (1) ◽  
pp. 59-63
Author(s):  
Dejan Spasovski ◽  
Todor Gruev ◽  
Nada Marina ◽  
Jordan Calovski ◽  
Ljubinka Rajčevska ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Diagnostic Markers ◽  
Border Region ◽  
Healthy Individuals ◽  
Kinetic Assay ◽  
Renal Lesions ◽  
Alanine Aminopeptidase ◽  
Glutamyl Transferase ◽  
Higher Sensitivity

Alanine Aminopeptidase, γ-Glutamyl Transferase and β2-microglobulin as Diagnostic Markers in Patients with Rheumatoid Arthritis The purpose of this research is to evaluate the values of alanine aminopeptidase (AAP), γ-glutamyl transferase (γ-GT), and β2-Microglobulin in urine (β2-M), in untreated rheumatoid arthritis (RA) and to define the effect of untreated rheumatoid arthritis on the tubular function and brush border region. We used a kinetic assay for AAP, standard methods by the IFCC for γ-glutamyl transferase and MEIA for the determination of β2-Microglobulin in urine in 70 participants (35 untreated RA patients, 35 healthy individuals). From the total of 35 RA patients, 24 patients had AAP (sensitivity of the test 68.57%), 16 patients had γ-GT enzymuria (sensitivity of the test 45.71%), while the presence of β2-Microglobulin in urine was found in a very low percentage. Out of 18 RF negative patients, 14 patients are AAP positive, 10 patients were γ-GT positive, while the presence of β2-Microglobulin in urine was not detected. Among 17 RF positive RA patients, the presence of AAP was noticed in 10, the presence of γ-GT in 6 patients, while the presence of β2-Microglobulin in urine was not detected. AAP has higher sensitivity than γ-GT and β2-Microglobulin in the detection of asymptomatic renal lesions in untreated RA.

Studies of Glutamate Dehydrogenase: Chemical Modification and Quantitative Determination of Tryptophan Residues

1974 ◽  
Vol 43 (2) ◽  
pp. 319-325 ◽  
Author(s):  
Veit Witzemann ◽  
Rudolf Koberstein ◽  
Horst Sund ◽  
Ihab Rasched ◽  
Hans Jornvall ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Quantitative Determination ◽  
Glutamate Dehydrogenase ◽  
Tryptophan Residues

Gamma-Glutamyl Transferase: Applications in Forensic Pathology: I: Study of Blood Serum Recovered from Human Bodies

1987 ◽  
Vol 27 (3) ◽  
pp. 152-160 ◽  
Author(s):  
M. Piette ◽  
G. De Schrijver
Keyword(s):  
Body Fluid ◽  
Sampling Site ◽  
Chronic Alcoholism ◽  
Gamma Glutamyl Transferase ◽  
Post Mortem ◽  
Chronic Alcohol ◽  
Gamma Glutamyl ◽  
Liver Degeneration ◽  
Glutamyl Transferase

In order to appreciate more adequately chronic alcohol use by the deceased in the course of a medico-legal autopsy we studied the usefulness of a determination of gamma-glutamyl transferase (GGT) activity in cadaver serum. Some preliminary tests were performed to check the post-mortem stability of this enzyme, the influence of post-mortem haemolysis upon enzyme determination, the influence of the sampling site upon the enzyme activity and the suitability of vitreous humour as an alternative body fluid for GGT determination. Secondly, correlations between GGT activity and the degree of fatty liver degeneration and fibrosis (both possibly alcohol-induced tissue lesions) were searched for. Finally, a comparison was made between strictly selected groups of alcoholics and non-alcoholics. We concluded that, keeping in mind the necessary precautions to avoid typical post-mortem fallacies, a GGT determination in cadaver serum provides a useful parameter for detecting chronic alcoholism at the forensic autopsy.

Determination of the chemical mechanism of glutamate dehydrogenase from pH studies

Biochemistry ◽  
1980 ◽  
Vol 19 (11) ◽  
pp. 2328-2333 ◽  
Author(s):  
James E. Rife ◽  
W. W. Cleland
Keyword(s):  
Glutamate Dehydrogenase ◽  
Chemical Mechanism

scholarly journals Determination of Blood Total, Reduced, and Oxidized Glutathione in Pediatric Subjects

2001 ◽  
Vol 47 (8) ◽  
pp. 1467-1469 ◽  
Author(s):  
Anna Pastore ◽  
Fiorella Piemonte ◽  
Mattia Locatelli ◽  
Anna Lo Russo ◽  
Laura Maria Gaeta ◽  
...  
Keyword(s):  
Oxidized Glutathione ◽  
Reduced And Oxidized Glutathione

Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer.

1976 ◽  
Vol 22 (10) ◽  
pp. 1614-1617 ◽  
Author(s):  
J P Bretaudiere ◽  
H T Phung ◽  
M Bailly
Keyword(s):  
Glutamate Dehydrogenase ◽  
Continuous Flow ◽  
Kinetic Scheme ◽  
Fixed Time ◽  
Sample Volume ◽  
Enzymatic Determination ◽  
Coupled Reactions ◽  
Continuous Flow Method ◽  
Enzymatic Micromethod

Abstract A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed-time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.

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