The polymerase chain reaction applied to identification of specific alleles of the bovine milk protein genes

1990 ◽  
Vol 18 (4) ◽  
pp. 675-676 ◽  
Author(s):  
STEPHEN J. PINDER ◽  
BARRY N. PERRY ◽  
DEMETRIS SAVVA ◽  
CHRISTOPHER J. SKIDMORE
Keyword(s):  
Polymerase Chain Reaction ◽  
Milk Protein ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Protein Genes ◽  
Polymerase Chain

scholarly journals Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

2013 ◽  
Vol 96 (11) ◽  
pp. 6955-6964 ◽  
Author(s):  
B.G. Botaro ◽  
C.S. Cortinhas ◽  
L.V. Março ◽  
J.F.G. Moreno ◽  
L.F.P. Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

scholarly journals Detection of Shigella in raw bovine milk by polymerase chain reaction

2020 ◽  
Vol 34 (1) ◽  
pp. 9-16
Author(s):  
Noor Soulieman ◽  
Aemaan Al-Mariri ◽  
Faizah Al-Atrash
Keyword(s):  
Polymerase Chain Reaction ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Polymerase Chain

Detection and Enumeration of Streptococcus agalactiae from Bovine Milk Samples by Real-Time Polymerase Chain Reaction

2015 ◽  
Vol 71 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Nara Ladeira de Carvalho ◽  
Juliano Leonel Gonçalves ◽  
Bruno Garcia Botaro ◽  
Luis Felipe de Prada e Silva ◽  
Marcos Veiga dos Santos
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

Polymerase Chain Reaction-Based DNA Fingerprinting for Identification of Streptococcus and Enterococcus Species Isolated from Bovine Milk

1994 ◽  
Vol 57 (3) ◽  
pp. 240-245 ◽  
Author(s):  
B. M. JAYARAO ◽  
S. P. OLIVER
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Fingerprinting ◽  
Optical Density ◽  
Bovine Milk ◽  
Oligonucleotide Primer ◽  
Dna Fragments ◽  
Enterococcus Species ◽  
Chain Reaction ◽  
Short Oligonucleotide ◽  
Polymerase Chain

A total of 353 isolates including 304 isolates from bovine milk belonging to 8 Streptococcus and Enterococcus species, 14 American Type Culture Collection reference strains, 10 isolates from bovine milk that had atypical biochemical profiles, and 25 bovine milk isolates from Vermont were examined by polymerase chain reaction-based DNA fingerprinting. A single short oligonucleotide primer of eight bases (5′-GTAACGCC-3′) was used to produce a characteristic spectrum of amplified DNA fragments. Amplified DNA fragments were visualized following agarose gel electrophoresis and analyzed by scanning laser densitometry. Amplified DNA fragments were categorized as either primary, secondary, or variable fragments. DNA fragments observed in all isolates within a species were designated as primary [optical density > 0.3 absorbance units (AU)] and secondary fragments (optical density > 0.15 AU), while infrequent DNA fragments were designated as variable fragments (optical density >0.15 AU). Examination of fingerprint patterns revealed that each of the species evaluated possessed a unique fingerprint profile. A simple identification scheme was developed based on the occurrence of primary and secondary DNA fragments for a species. The polymerase chain reaction-based DNA fingerprinting assay was simple to perform, reliable, and reproducible. Results of this study indicate that DNA fingerprinting using polymerase chain reaction has the potential to be developed as a routine method for species identification of bacteria.

scholarly journals Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction

2010 ◽  
Vol 79 (2) ◽  
pp. 277-280 ◽  
Author(s):  
Ayman Al-Mariri ◽  
Nermeen Haj-Mahmoud
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Brucella Abortus ◽  
Bovine Milk ◽  
Ring Test ◽  
Chain Reaction ◽  
Purification System ◽  
Polymerase Chain ◽  
Direct Dna Extraction ◽  
Milk Ring Test

This study compares milk ring test and three different polymerase chain reaction techniques (direct DNA extraction by column purification system, alkaline DNA extraction, and filtrated milk), in order to identifyBrucella abortusinfection in bovine milk. Milk ring test sensitivity and specificity were 72% and 80%, respectively. While specificity of the three polymerase chain reaction techniques was 100%; sensitivity was 92%, 88% and 100%, respectively, for the three polymerase chain reaction procedures. We conclude that the filtered animal’s milk polymerase chain reaction is the best procedure to make the diagnosis ofB. abortusinfections.

Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese

2001 ◽  
Vol 68 (4) ◽  
pp. 689-698 ◽  
Author(s):  
STEFANO REA ◽  
KOICHI CHIKUNI ◽  
RAFFAELLA BRANCIARI ◽  
RAM SUKASI SANGAMAYYA ◽  
DAVID RANUCCI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Species Identification ◽  
Water Buffalo ◽  
Bovine Milk ◽  
Buffalo Milk ◽  
Animal Origin ◽  
Duplex Pcr ◽  
Mozzarella Cheese ◽  
Chain Reaction ◽  
Polymerase Chain

Molecular biology techniques have been used for species identification in food of animal origin in relatively recent years. A polymerase chain reaction (PCR) based method, the multiplex PCR, was recently applied to species identification in meat and meat products. It allows co-amplification of separate regions of a single gene or specific fragments, each typical of a different animal species in a single PCR reaction, using different pairs of primers in the same reaction mix. In the present paper, the duplex-PCR technique is proposed to identify bovine and water buffalo DNA in a single PCR assay in milk and mozzarella cheese (a typical Italian cheese, originally made from pure water buffalo milk). Because of its lower cost, undeclared bovine milk is added to water buffalo milk for making different kinds of mozzarella cheese. The results of this experiment indicate the applicability of this method, which showed an absolute specificity for the two species and a high sensitivity even down to low DNA concentrations (1 pg). In bovine and water buffalo mixtures of both milk and mozzarella cheese, the minimum concentration tested was 1% of bovine in water buffalo milk and water buffalo in bovine milk. The importance of the somatic cell content in raw milk is also discussed with special reference to the evaluation of mixtures (milk or cheese) of the two species.

scholarly journals Detection of Listeria monocytogenes by the polymerase chain reaction (PCR) technique in samples of raw bovine milk

2012 ◽  
Vol 67 (389) ◽  
pp. 15-20
Author(s):  
Camila Agostini ◽  
Caroline Schwertner Kreling ◽  
Ivan Cunha Bustamante-Filho ◽  
Cláucia Fernanda Volken de Souza ◽  
Vanderlei Biolchi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction

2015 ◽  
Vol 8 (7) ◽  
pp. 913-917 ◽  
Author(s):  
Eyman Y. Osman ◽  
A. M. S. El-Eragi ◽  
Abuobeida M. Musa ◽  
Salma B. El-Magboul ◽  
Magdi B. A/Rahman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Helicobacter Pylori ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain
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