Plasma lipoprotein profiles and the distribution of high-density lipoprotein subfractions in the elderly: the effect of Alzheimer's disease and multi-infarct dementia

1990 ◽  
Vol 18 (2) ◽  
pp. 324-324 ◽  
Author(s):  
ANTON VAN RHIJN ◽  
FIONA MACINTYRE ◽  
FRANK M. CORRIGAN ◽  
CAROLYN WATT ◽  
GEOFFREY IJOMAH ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
The Elderly ◽  
High Density ◽  
Plasma Lipoprotein ◽  
Lipoprotein Subfractions ◽  
High Density Lipoprotein Subfractions ◽  
Lipoprotein Profiles

High-density lipoprotein subfractions measured in stored serum

1994 ◽  
Vol 40 (9) ◽  
pp. 1713-1716 ◽  
Author(s):  
L L Bausserman ◽  
A L Saritelli ◽  
D Milosavljevic
Keyword(s):  
High Density Lipoprotein ◽  
Dextran Sulfate ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Manganese Chloride ◽  
Lipoprotein Subfractions ◽  
Hdl Subfractions ◽  
High Density Lipoprotein Subfractions

Abstract We compared the effects of freezing serum on the determination of high-density lipoprotein (HDL) subfractions by two dual-precipitation methods, heparin and manganese chloride/dextran sulfate (HM/DS) (Gidez et al., J Lipid Res 1982;23:1206-23) and DS/DS (Warnick et al., Clin Chem 1982;28:1574), and by ultracentrifugation. Storing serum for 1 month at -70 degrees C resulted in reduced HDL3-cholesterol by ultracentrifugation and reduced total and HDL3-cholesterol by the DS/DS method. There was no change in either total HDL-cholesterol or HDL3-cholesterol with the HM/DS method. Additional studies involving only HM/DS indicated that total HDL-cholesterol in serum stored at 4 degrees C begins to decline after 3 days (-3.1 +/- 3.5%, P < 0.1). HDL was stable at -20 degrees C for 2 weeks but both total and HDL3-cholesterol decreased significantly after 1 month. Storage of serum at -70 degrees C resulted in no changes for 1 year; however, at 18 months, HDL3-cholesterol was reduced 13% (P = 0.002). We conclude that HDL subfractions can be determined accurately in serum as well as in plasma after storage at -70 degrees C for up to 1 year.

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