Observations of the fusion and haemolytic activity of influenza virus by digital fluorescence imaging

1989 ◽  
Vol 17 (6) ◽  
pp. 1099-1100 ◽  
Author(s):  
GEORGE N. GIORGIOU ◽  
IAN E. G. MORRISON ◽  
RICHARD J. CHERRY
Keyword(s):  
Influenza Virus ◽  
Fluorescence Imaging ◽  
Haemolytic Activity ◽  
Digital Fluorescence Imaging

scholarly journals Digital fluorescence imaging of fusion of influenza virus with erythrocytes

FEBS Letters ◽  
1989 ◽  
Vol 250 (2) ◽  
pp. 487-492 ◽  
Author(s):  
George N. Georgiou ◽  
Ian E.G. Morrison ◽  
Richard J. Cherry
Keyword(s):  
Influenza Virus ◽  
Fluorescence Imaging ◽  
Digital Fluorescence Imaging

scholarly journals Lateral Distribution of the Transmembrane Domain of Influenza Virus Hemagglutinin Revealed by Time-resolved Fluorescence Imaging

2009 ◽  
Vol 284 (23) ◽  
pp. 15708-15716 ◽  
Author(s):  
Silvia Scolari ◽  
Stephanie Engel ◽  
Nils Krebs ◽  
Anna Pia Plazzo ◽  
Rodrigo F. M. De Almeida ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Fluorescence Imaging ◽  
Transmembrane Domain ◽  
Lateral Distribution ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Influenza Virus Hemagglutinin

Digital Fluorescence Imaging of Trafficking of Endosomes Containing Low-Density Lipoprotein in Brain Astroglial Cells

2000 ◽  
Vol 269 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Tomomitsu Ichikawa ◽  
Makoto Yamada ◽  
Daisaku Homma ◽  
Richard J. Cherry ◽  
Ian E.G. Morrison ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Astroglial Cells ◽  
Digital Fluorescence Imaging

scholarly journals Fluorescence Imaging of Influenza Virus H1N1 mRNA in Living Infected Cells using Single Chromophore FIT-PNA

2011 ◽  
Vol 100 (3) ◽  
pp. 182a-183a ◽  
Author(s):  
Susann Kummer ◽  
Andrea Knoll ◽  
Elke Socher ◽  
Lucas Bethge ◽  
Andreas Herrmann ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Fluorescence Imaging ◽  
Virus H1n1 ◽  
Infected Cells

DIGITAL FLUORESCENCE IMAGING OF ORGANIC CATION TRANSPORT IN FRESHLY ISOLATED RAT PROXIMAL TUBULES

2005 ◽  
Vol 34 (3) ◽  
pp. 339-342 ◽  
Author(s):  
Frank Pietruck ◽  
Markus Horbelt ◽  
Thorsten Feldkamp ◽  
Katrin Engeln ◽  
Stefan Herget-Rosenthal ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Organic Cation ◽  
Cation Transport ◽  
Proximal Tubules ◽  
Organic Cation Transport ◽  
Digital Fluorescence Imaging ◽  
Isolated Rat

Comparitive analysis of MHC class I and class II cell surface organisation using digital fluorescence imaging

1996 ◽  
Vol 47 (1-2) ◽  
pp. 96
Author(s):  
Patricia R. Smith ◽  
Keith M. Wilson ◽  
Ian E.G. Morrison ◽  
Nelson Fernandez ◽  
Richard J. Cherry
Keyword(s):  
Cell Surface ◽  
Fluorescence Imaging ◽  
Mhc Class I ◽  
Class Ii ◽  
Class I ◽  
Digital Fluorescence Imaging

scholarly journals Cellular uptake and intracellular localization of benzo(a)pyrene by digital fluorescence imaging microscopy.

1985 ◽  
Vol 100 (4) ◽  
pp. 1295-1308 ◽  
Author(s):  
A L Plant ◽  
D M Benson ◽  
L C Smith
Keyword(s):  
Fluorescence Imaging ◽  
Cellular Uptake ◽  
Intracellular Localization ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Intracellular Location ◽  
Fluorescence Imaging Microscopy ◽  
Digital Fluorescence Imaging

Uptake of benzo(a)pyrene by living cultured cells has been visualized in real time using digital fluorescence-imaging microscopy. Benzo(a)pyrene was noncovalently associated with lipoproteins, as a physiologic mode of presentation of the carcinogen to cells. When incubated with either human fibroblasts or murine P388D1 macrophages, benzo(a)pyrene uptake occurred in the absence of endocytosis, with a halftime of approximately 2 min, irrespective of the identity of the delivery vehicles, which were high density lipoproteins, low density lipoproteins, very low density lipoproteins, and 1-palmitoyl-2-oleoylphosphatidylcholine single-walled vesicles. Thus, cellular uptake of benzo(a)pyrene from these hydrophobic donors occurs by spontaneous transfer through the aqueous phase. Moreover, the rate constant for uptake, the extent of uptake, and the intracellular localization of benzo(a)pyrene were identical for both living and fixed cells. Similar rate constants for benzo(a)pyrene efflux from cells to extracellular lipoproteins suggests the involvement of the plasma membrane in the rate-limiting step. The intracellular location of benzo(a)pyrene at equilibrium was coincident with a fluorescent cholesterol analog, N-(7-nitrobenz-2-oxa-1,3-diazole)-23,24-dinor-5-cholen-22-amine-3 beta-ol. Benzo(a)pyrene did not accumulate in acidic compartments, based on acridine orange fluorescence, or in mitochondria, based on rhodamine-123 fluorescence. When the intracellular lipid volume of isolated mouse peritoneal macrophages was increased by prior incubation of these cells with either acetylated low density lipoproteins or with very low density lipoproteins from a hypertriglyceridemic individual, cellular accumulation of benzo(a)pyrene increased proportionately with increased [1-14C]oleate incorporation into cellular triglycerides and cholesteryl esters. Thus, benzo(a)pyrene uptake by cells is a simple partitioning phenomenon, controlled by the relative lipid volumes of extracellular donor lipoproteins and of cells, and does not involve lipoprotein endocytosis as an obligatory step.

Digital fluorescence imaging of elementary steps of neurosteroid synthesis in rat brain glial cells

1997 ◽  
Vol 15 (9-10) ◽  
pp. 1231-1240 ◽  
Author(s):  
Tetsuya Kimoto ◽  
Hiroaki Asou ◽  
Yoshihiro Ohta ◽  
Hideo Mukai ◽  
Alexey A. Chernogolov ◽  
...  
Keyword(s):  
Rat Brain ◽  
Fluorescence Imaging ◽  
Glial Cells ◽  
Elementary Steps ◽  
Digital Fluorescence Imaging

Detection and Isolation of a Drug-Resistant Influenza Virus Using a Sialidase Fluorescence Imaging Technique

2020 ◽  
Vol 32 (186) ◽  
pp. J35-J41
Author(s):  
Yuuki Kurebayashi ◽  
Tadanobu Takahashi ◽  
Tadamune Otsubo ◽  
Akira Minami ◽  
Kiyoshi Ikeda ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Fluorescence Imaging ◽  
Imaging Technique ◽  
Drug Resistant
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