Rat liver protein synthesis after partial hepatectomy

1989 ◽  
Vol 17 (6) ◽  
pp. 1047-1047 ◽  
Author(s):  
V. R. PREEDY ◽  
L. PASKA ◽  
P. H. SUGDEN ◽  
P. S. SCHOFIELD ◽  
M. C. SUGDEN
Keyword(s):  
Protein Synthesis ◽  
Partial Hepatectomy ◽  
Rat Liver ◽  
Liver Protein

scholarly journals Polyribosomes in isolated liver cells. Preparative procedures, effects of incubation and correlation with protein synthesis

1980 ◽  
Vol 186 (1) ◽  
pp. 35-45 ◽  
Author(s):  
A J Dickson ◽  
C I Pogson
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Isolated Hepatocytes ◽  
Liver Cells ◽  
Liver Protein ◽  
Isolated Cells ◽  
Isolated Liver Cells ◽  
Rat Liver Cells ◽  
Isolated Liver

Methods have been derived which permit the isolation of undergraded polyribosomes from isolated rat liver cells. Under the conditions used the polyribosome profile of hepatocytes immediately after isolation was essentially identical with that from intact liver. However, during incubation of cells in complex physiological media there was a progressive dissociation of polyribosomes. The addition of a variety of factors that produce reaggregation of polyribosomes in rat liver in vivo did not prevent dissociation during cell incubations. Although large polyribosomes were lost most rapidly, the albumin-synthesizing capacity of isolated cells was not selectively lost when compared with total protein synthesis. The significance of these results for the use of isolated hepatocytes in the study of liver protein synthesis is discussed.

scholarly journals Inhibition of polyamine accumulation and deoxyribonucleic acid synthesis in regenerating rat liver

1976 ◽  
Vol 158 (2) ◽  
pp. 485-488 ◽  
Author(s):  
H Pösö ◽  
J Jänne
Keyword(s):  
Partial Hepatectomy ◽  
Ornithine Decarboxylase ◽  
Rat Liver ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Liver Protein ◽  
Polyamine Synthesis ◽  
Total Liver ◽  
Regenerating Rat Liver ◽  
Deoxyribonucleic Acid Synthesis

Repeated injections of 1,3-diaminopropane into rats after partial hepatectomy caused a repression-type inhibiton of liver ornithine decarboxylase (EC 4.1.1.17) and totally prevented the marked increases in liver putrescine and spermidine concentrations that normally occur in response to partial hepatectomy. The inhibition of polyamine synthesis by diaminopropane was accompanied by a profound decrease (about 80%) in the synthesis of DNA in the regenerating rat liver without any changes in the synthesis of RNA and total liver protein.

scholarly journals The effect of starvation on the rate of protein synthesis in rat liver and small intestine

1979 ◽  
Vol 178 (2) ◽  
pp. 373-379 ◽  
Author(s):  
M A McNurlan ◽  
A M Tomkins ◽  
P J Garlick
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Small Intestine ◽  
Rat Liver ◽  
Specific Radioactivity ◽  
Jejunal Mucosa ◽  
Liver Protein ◽  
Total Liver

1. A method is described that allows for measurement of protein synthesis in liver and intestine in the rat. By injecting a massive amount of [14C]leucine (100 mumol/100 g body wt.) an attempt has been made to over come problems of precursor specific radioactivity and problems arising from the breakdown of labelled protein that are encountered when tracer amounts of amino acids are used. 2. Starvation for 2 days resulted in decline in the rate of total liver protein synthesis from 87%/day to 62%/day. 3. In jejunal mucosa the rate of protein synthesis was 136%/day. This declined to 105%/day after 2 days of starvation.

scholarly journals The effect of dietary protein deficiency on albumin synthesis and on the concentration of active albumin messenger ribonucleic acid in rat liver

1978 ◽  
Vol 172 (1) ◽  
pp. 129-135 ◽  
Author(s):  
V M Pain ◽  
M J Clemens ◽  
P J Garlick
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Dietary Protein ◽  
Liver Protein ◽  
Albumin Synthesis ◽  
Deficient Diet ◽  
Messenger Ribonucleic Acid ◽  
Cytoplasmic Rna ◽  
Reticulocyte Lysate ◽  
Albumin Mrna

In rats fed on a protein-deficient diet, albumin synthesis as a percentage of total liver protein synthesis falls from the normal value of approx. 15% to about 8%. We have extracted total cytoplasmic RNA from individual rat livers and measured the concentration of active albumin mRNA by translation in a reticulocyte lysate system from which the endogenous mRNA had been removed [Pelham & Jackson (1976) Eur. J. Biochem. 67, 247-256]. In this messenger-dependent system it is possible to measure the synthesis of albumin as a proportion of the overall protein synthesis promoted by the addition of the hepatic RNA. The results show that the concentration of translatable albumin mRNA in samples of total cytoplasmic RNA from livers of protein-deficient rats is decreased markedly. These findings suggest that dietary protein supply affects selectively the synthesis and/or functional stability of albumin mRNA in rat liver.

scholarly journals Liver Protein Synthesis after Partial Hepatectomy and Acute Stress

1967 ◽  
Vol 242 (4) ◽  
pp. 700-704 ◽  
Author(s):  
C. Majumdar ◽  
Kinji Tsukada ◽  
Irving Lieberman
Keyword(s):  
Protein Synthesis ◽  
Partial Hepatectomy ◽  
Acute Stress ◽  
Liver Protein

Glucagon effect on rat liver protein synthesis in vivo

1973 ◽  
Vol 52 (2) ◽  
pp. 582-587 ◽  
Author(s):  
Matilde S. Ayuso-Parrilla ◽  
Roberto Parrilla
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Liver Protein

The acute effects of N-hydroxy-acetylaminofluorene on rat liver protein synthesis

Life Sciences ◽  
1979 ◽  
Vol 25 (17) ◽  
pp. 1477-1487 ◽  
Author(s):  
Geoffrey H. Moyer ◽  
Garth E. Austin
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Liver Protein ◽  
Acute Effects
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