β-Ketoacyl-acyl-carrier protein synthase from developing seeds of oilseed rape (Brassica napus)

1989 ◽  
Vol 17 (4) ◽  
pp. 686-687 ◽  
Author(s):  
ROBERT W. MACKINTOSH ◽  
D. GRAHAME HARDIE ◽  
ANTONI R. SLABAS
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Developing Seeds

scholarly journals Common themes in redox chemistry emerge from the X-ray structure of oilseed rape (Brassica napus) enoyl acyl carrier protein reductase

Structure ◽  
1995 ◽  
Vol 3 (9) ◽  
pp. 927-938 ◽  
Author(s):  
John B Rafferty ◽  
J.William Simon ◽  
Clair Baldock ◽  
Peter J Artymiuk ◽  
Patrick J Baker ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Acyl Carrier Protein ◽  
Redox Chemistry ◽  
Carrier Protein ◽  
X Ray

Acyl–acyl carrier protein (ACP)-thioesterase from developing seeds of Brassica campestris cv. B-54 (Agrani)

Plant Science ◽  
2004 ◽  
Vol 166 (1) ◽  
pp. 191-198 ◽  
Author(s):  
Manas K Pathak ◽  
Ashish Bhattacharjee ◽  
Dolly Ghosh ◽  
Sudhamoy Ghosh
Keyword(s):  
Brassica Campestris ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Developing Seeds

cDNA cloning and expression of Brassica napus enoyl-acyl carrier protein reductase in Escherichia coli

1991 ◽  
Vol 17 (4) ◽  
pp. 895-909 ◽  
Author(s):  
Martin M. Kater ◽  
Gregory M. Koningstein ◽  
H. John J. Nijkamp ◽  
Antoine R. Stuitje
Keyword(s):  
Escherichia Coli ◽  
Brassica Napus ◽  
Cdna Cloning ◽  
Acyl Carrier Protein ◽  
Cloning And Expression ◽  
Carrier Protein

scholarly journals Overexpression of Acyl-ACP Thioesterases, CpFatB4 and CpFatB5, Induce Distinct Gene Expression Reprogramming in Developing Seeds of Brassica napus

2019 ◽  
Vol 20 (13) ◽  
pp. 3334 ◽  
Author(s):  
Jeong-Won Nam ◽  
Jinouk Yeon ◽  
Jiseong Jeong ◽  
Eunyoung Cho ◽  
Ho Bang Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Transgenic Plants ◽  
Palmitic Acid ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Rna Seq ◽  
Oleate Desaturase ◽  
Fa Composition ◽  
Genetic Modifications

We examined the substrate preference of Cuphea paucipetala acyl-ACP thioesterases, CpFatB4 and CpFatB5, and gene expression changes associated with the modification of lipid composition in the seed, using Brassica napus transgenic plants overexpressing CpFatB4 or CpFatB5 under the control of a seed-specific promoter. CpFatB4 seeds contained a higher level of total saturated fatty acid (FA) content, with 4.3 times increase in 16:0 palmitic acid, whereas CpFatB5 seeds showed approximately 3% accumulation of 10:0 and 12:0 medium-chain FAs, and a small increase in other saturated FAs, resulting in higher levels of total saturated FAs. RNA-Seq analysis using entire developing pods at 8, 25, and 45 days after flowering (DAF) showed up-regulation of genes for β-ketoacyl-acyl carrier protein synthase I/II, stearoyl-ACP desaturase, oleate desaturase, and linoleate desaturase, which could increase unsaturated FAs and possibly compensate for the increase in 16:0 palmitic acid at 45 DAF in CpFatB4 transgenic plants. In CpFatB5 transgenic plants, many putative chloroplast- or mitochondria-encoded genes were identified as differentially expressed. Our results report comprehensive gene expression changes induced by alterations of seed FA composition and reveal potential targets for further genetic modifications.

Recombinant antibody fragments that detect enoyl acyl carrier protein reductase in Brassica napus

Lipids ◽  
1997 ◽  
Vol 32 (8) ◽  
pp. 805-809 ◽  
Author(s):  
Angelika Ziegler ◽  
Sybil M. Macintosh ◽  
Lesley Torrance ◽  
William Simon ◽  
Antony R. Slabas
Keyword(s):  
Brassica Napus ◽  
Acyl Carrier Protein ◽  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Carrier Protein ◽  
Recombinant Antibody Fragments
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