Plant host-pathogen interaction: elicitation of phenylalanine ammonia lyase activity and its mediation by Ca2+

1988 ◽  
Vol 16 (6) ◽  
pp. 1069-1070 ◽  
Author(s):  
CHRISTOPHER J. SMITH ◽  
RUSSELL P. NEWTON ◽  
CAROL J. MULLINS ◽  
TERENCE J. WALTON
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Plant Host ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
Lyase Activity ◽  
Phenylalanine Ammonia Lyase Activity

Effects of Herbicides on Growth and Extractable Phenylalanine Ammonia-Lyase Activity in Light- and Dark-Grown Soybean (Glycine max) Seedlings

Weed Science ◽  
1981 ◽  
Vol 29 (4) ◽  
pp. 433-439 ◽  
Author(s):  
Robert E. Hoagland ◽  
Stephen O. Duke
Keyword(s):  
Glycine Max ◽  
Phenylalanine Ammonia Lyase ◽  
Dry Weight ◽  
Root Tip ◽  
Continuous Darkness ◽  
Soybean Seedlings ◽  
Pal Activity ◽  
Lyase Activity ◽  
Phenylalanine Ammonia Lyase Activity

Effects of 16 herbicides representing 14 herbicide classes on growth and extractable phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) were examined in light- and dark-grown soybean [Glycine max(L.) Merr. ‘Hill’] seedlings. High purity (96 to 100%) herbicides were supplied via aqueous culture at various concentrations: 0.5 mM amitrole (3-amino-s-triazole), 0.1 mM atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine], 0.07 mM diclofop-methyl {methyl ester of 2-[4-(2,4-dichlorophenoxy)phenoxy] propanoicacid}, 0.5 mM DSMA (disodium methanearsonate), 0.2 mM fenuron (1,1-dimethyl-3-phenylurea), 0.05 mM fluridone {1-methyl-3-phenyl-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone}, 0.5 mM MH (1,2-dihydro-3,6-pyridazinedione), 0.5 mM metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one], 1.8 μM nitralin [4-(methylsulfonyl)-2,6-dinitro-N,N-dipropylaniline], 0.5 mM norflurazon [4-chloro-5-(methylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], 0.05 mM paraquat (1,1′-dimethyl-4,4′-bipyridinium ion), 0.15 mM perfluidone {1,1,1-trifluoro-N-[2-methyl-4-(phenylsulfonyl)phenyl] methanesulfonamide}, 0.2 mM propanil (3′,4′-dichloropropionanilide), 0.1 mM propham (isopropyl carbanilate), 0.5 mM TCA (trichloroacetic acid), and 0.05 mM 2,4-D [(2,4-dichlorophenoxy)acetic acid]. Dark-grown soybean seedlings (3-day-old) were transferred to control solutions (2 mM CaSO4) or to herbicide solutions (in 2 mM CaSO4) and grown at 25 C in continuous white light (200 μE•m-2•s-1) or continuous darkness until harvested 24 or 48 h after transfer. After 48 h, growth (fresh weight, dry weight, elongation) was inhibited by most of the chemicals. Other signs of toxicity (necrosis, secondary root stunting, and root tip swelling) were noted for some treatments. Roots were most affected, although hypocotyls were generally not changed. Hypocotyl elongation was stimulated by atrazine, fluridone, and norflurazon after 48 h light. Extractable PAL activity from soybean axes was decreased by atrazine, fenuron, metribuzin, norflurazon, propanil, propham, and 2,4-D. Amitrole and paraquat were the only herbicides that increased extractable PAL activity. Other compounds tested had no effect on the enzyme. None of the herbicides significantly affected in vitro PAL activity.

scholarly journals Changes in phenols composition and activity of phenylalanine-ammonia lyase in apples after fungal infections

2011 ◽  
Vol 38 (No. 1) ◽  
pp. 1-10 ◽  
Author(s):  
J. Schovánková ◽  
H. Opatová
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Fungal Infections ◽  
Penicillium Expansum ◽  
Total Phenol Content ◽  
Fruit Peel ◽  
Defensive Reaction ◽  
Monilinia Fructigena ◽  
Lyase Activity ◽  
Phenylalanine Ammonia Lyase Activity ◽  
Healthy Part

The defensive reaction of apple cultivar Idared (Malus domestica Borkh.) was studied after inoculation with three different pathogens (Penicillium expansum, Monilinia fructigena, and Gloeosporium spp.). Changes in phenolic content and activity of phenylalanine-ammonia lyase were determined after 7, 14, and 21 days after the inoculation. The significant differences were discovered in the progress of rotting after the inoculation. The increase in phenols concentration and in phenylalanine-ammonia lyase activity varied in the place of fungal attack, in the tissues around rotten zone and in the healthy part. The response to the infection was different in the fruit peel and flesh. Very good correlation was found between the activity of phenylalanine-ammonia lyase and total phenol content (r = 0.76–0.98).

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