Differences in the degradation in vivo and in vitro of phaseolin, the major storage protein of Phaseolus vulgaris seeds

1988 ◽  
Vol 16 (4) ◽  
pp. 612-613 ◽  
Author(s):  
LUIZ G. SANTORO ◽  
GEORGE GRANT ◽  
ARPAD PUSZTAI
Keyword(s):  
Phaseolus Vulgaris ◽  
Storage Protein ◽  
Major Storage Protein

THE MAJOR STORAGE PROTEIN OF FRENCH BEAN SEEDS: CHARACTERIZATION IN VIVO AND TRANSLATION IN VITRO

1979 ◽  
pp. 3-26 ◽  
Author(s):  
T.C. Hall ◽  
S.M. Sun ◽  
Y. Ma ◽  
R.C. McLeester ◽  
J.W. Pyne ◽  
...  
Keyword(s):  
Storage Protein ◽  
French Bean ◽  
Major Storage Protein

scholarly journals Cell-free Synthesis of the Major Storage Protein of the Bean, Phaseolus vulgaris L.

1975 ◽  
Vol 56 (6) ◽  
pp. 780-785 ◽  
Author(s):  
Sai-Ming M. Sun ◽  
Barry U. Buchbinder ◽  
Timothy C. Hall
Keyword(s):  
Phaseolus Vulgaris ◽  
Storage Protein ◽  
Phaseolus Vulgaris L ◽  
Major Storage Protein

scholarly journals Identification of glycinin in vivo as a polyamine-conjugated protein via a γ-glutamyl linkage

1998 ◽  
Vol 332 (2) ◽  
pp. 467-473 ◽  
Author(s):  
Hyeog KANG ◽  
Seung Gwan LEE ◽  
Young Dong CHO
Keyword(s):  
Storage Protein ◽  
Specific Activity ◽  
Catalytic Action ◽  
Soybean Seeds ◽  
Free Polyamines ◽  
Major Storage Protein

To identify a polyamine-conjugated protein by the action of transglutaminase in the absence of radiolabelled polyamine, extracts prepared from the leaves and developing soybean seeds were investigated for the specific activity of transglutaminase and the content of free polyamines. We identified the major storage protein, glycinin, as a polyamine-conjugated protein. This was established by the following procedures: (1) immunolocalization with antibody against putrescine prepared in rabbit against putrescine–BSA conjugate; (2) immunocross-reactivity on nitrocellulose transblot of the purified glycinin subunits by using antibody against putrescine; (3) identification of polyamines in acid hydrolysates of purified glycinin; (4) release of polyamines in proteolytic digests through the catalytic action of γ-glutamylamine cyclotransferase, an enzyme specific for the disassembly of γ-glutamylamines. The activity of γ-glutamylamine cyclotransferase was also identified in soybean seeds.

The Metabolism of Estrogens in vivo and in vitro by Phaseolus vulgaris

1979 ◽  
Vol 94 (4) ◽  
pp. 307-316 ◽  
Author(s):  
I.J. Young ◽  
B.A. Knights ◽  
J.R. Hillman
Keyword(s):  
Phaseolus Vulgaris

scholarly journals Heritable Variation in a Polypeptide Subunit of the Major Storage Protein of the Bean, Phaseolus vulgaris L.

1975 ◽  
Vol 56 (6) ◽  
pp. 776-779 ◽  
Author(s):  
Jeanne Romero ◽  
Sai-Ming M. Sun ◽  
Robert C. McLeester ◽  
Fredick A. Bliss ◽  
Timothy C. Hall
Keyword(s):  
Phaseolus Vulgaris ◽  
Storage Protein ◽  
Phaseolus Vulgaris L ◽  
Heritable Variation ◽  
Major Storage Protein
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