Effects of Ruthenium Red on O2 uptake and lactate and CO2 production by the perfused rat heart in the absence and presence of isoprenaline

1988 ◽  
Vol 16 (4) ◽  
pp. 591-592
Author(s):  
JOHN F. UNITT ◽  
JAMES G. McCORMACK ◽  
PAUL J. ENGLAND
Keyword(s):  
Rat Heart ◽  
Ruthenium Red ◽  
Co2 Production ◽  
O2 Uptake ◽  
Perfused Rat Heart

scholarly journals Ruthenium Red inhibits the activation of pyruvate dehydrogenase caused by positive inotropic agents in the perfused rat heart

1983 ◽  
Vol 214 (2) ◽  
pp. 581-585 ◽  
Author(s):  
J G McCormack ◽  
P J England
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Rat Heart ◽  
Contractile Force ◽  
Ruthenium Red ◽  
Inotropic Agents ◽  
Similar Increase ◽  
Perfused Rat Heart ◽  
Control Value ◽  
Positive Inotropic Agents ◽  
Total Enzyme

The increases in the amount of active, non-phosphorylated, pyruvate dehydrogenase caused by positive inotropic agents (from a control value of about 10%, to 40% of total enzyme) in the perfused rat heart could be completely blocked by prior perfusion with 2.5 micrograms of Ruthenium Red/ml. A similar increase caused by 5 mM-pyruvate was not blocked. This concentration of Ruthenium Red caused a 25% decrease in contractile force of hearts perfused in the absence of positive inotropic agents; however, in their presence the contractile force reached the same value in the absence or presence of Ruthenium Red. Neither control nor stimulated phosphorylase a content was affected by Ruthenium Red. Verapamil (0.1 microM) also decreased control contraction (by 40%), but did not block the activation of pyruvate dehydrogenase caused by a rise in extracellular [Ca2+]. The results support the hypothesis that positive inotropic agents activate pyruvate dehydrogenase in rat heart by increasing intramitochondrial [Ca2+].

scholarly journals Pseudoketogenesis in the perfused rat heart.

1988 ◽  
Vol 263 (34) ◽  
pp. 18036-18042 ◽  
Author(s):  
G Fink ◽  
S Desrochers ◽  
C Des Rosiers ◽  
M Garneau ◽  
F David ◽  
...  
Keyword(s):  
Rat Heart ◽  
Perfused Rat Heart
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