Bile acid synthesis by human hepatoma cell line Hep-G2 is stimulated by low density lipoprotein

1988 ◽  
Vol 16 (4) ◽  
pp. 566-567
Author(s):  
HERMAN JAN KEMPEN ◽  
ELLEN LOMMERSE ◽  
RINA VAN SCHIE ◽  
ELLEN HEPP ◽  
PIET MEYER ◽  
...  
Keyword(s):  
Hepatoma Cell ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Acid Synthesis ◽  
Bile Acid Synthesis ◽  
Hepatoma Cell Line ◽  
Low Density ◽  
Human Hepatoma ◽  
Hep G2 ◽  
Human Hepatoma Cell

scholarly journals Low Density Lipoprotein (LDL) Receptors Expressed on a Human Hepatoma Cell Line, Mahlavu

1989 ◽  
Vol 17 (6) ◽  
pp. 925-930
Author(s):  
Naohiko SAKAI ◽  
Shizuya YAMASHITA ◽  
Yuhya UEYAMA ◽  
Masakazu MENJU ◽  
Toshiharu KAWAMOTO ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatoma Cell ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hepatoma Cell Line ◽  
Low Density ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Ldl Receptors ◽  
Human Hepatoma Cell Line

scholarly journals Effects of compactin, mevalonate and low-density lipoprotein on 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and low-density-lipoprotein-receptor activity in the human hepatoma cell line Hep G2

1984 ◽  
Vol 222 (1) ◽  
pp. 35-39 ◽  
Author(s):  
L H Cohen ◽  
M Griffioen ◽  
L Havekes ◽  
D Schouten ◽  
V van Hinsbergh ◽  
...  
Keyword(s):  
Reductase Activity ◽  
Hepatoma Cell ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hepatoma Cell Line ◽  
Low Density ◽  
Hep G2 ◽  
Human Hepatoma Cell ◽  
Hmg Coa Reductase ◽  
Coa Reductase

Compactin, an inhibitor of HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase, decreased cholesterol synthesis in intact Hep G2 cells. However, after the inhibitor was washed away, the HMG-CoA-reductase activity determined in the cell homogenate was found to be increased. Also the high-affinity association of LDL (low-density lipoprotein) to Hep G2 cells was elevated after incubation with compactin. Lipoprotein-depleted serum, present in the incubation medium, potentiated the compactin effect compared with incubation in the presence of human serum albumin. Addition of either mevalonate or LDL prevented the compactin-induced rise in activities of both HMG-CoA reductase and LDL receptor in a comparable manner. It is concluded that in this human hepatoma cell line, as in non-transformed cells, both endogenous mevalonate or mevalonate-derived products and exogenous cholesterol are able to modulate the HMG-CoA reductase activity as well as the LDL-receptor activity.

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