A semi-automated method for the determination of pepsin activity in gastric juice using the Tecan liquid-handling system

1988 ◽  
Vol 16 (1) ◽  
pp. 26-27
Author(s):  
ANDREW G. LYNN ◽  
ANTHONY C. LANE ◽  
LYNDA A. SMEATON
Keyword(s):  
Gastric Juice ◽  
Pepsin Activity ◽  
Handling System ◽  
Liquid Handling ◽  
Automated Method

An Automated Method for the Determination of Serum Calcium with Glyoxal Bis (2-Hydroxyanil)

1967 ◽  
Vol 13 (6) ◽  
pp. 515-520 ◽  
Author(s):  
Genevieve Farese ◽  
Janice L Schmidt ◽  
Milton Mager
Keyword(s):  
Serum Calcium ◽  
Automated Analysis ◽  
Automated Method ◽  
Good Agreement

Abstract A completely automated analysis is described for the determination of serum calcium with glyoxal bis (2-hydroxyanil) solution (GBHA). The method is simple and precise, and the data obtained are in good agreement with results obtained by the manual GBHA procedure.

scholarly journals Automated method for the colorimetric determination of acyl esters in serum

1965 ◽  
Vol 6 (2) ◽  
pp. 301-306
Author(s):  
Arnold Antonis ◽  
D.S. Platt ◽  
J.M. Thorp
Keyword(s):  
Colorimetric Determination ◽  
Automated Method

scholarly journals An HPLC-automated Derivatization for Glutathione and Related Thiols Analysis in Brassica rapa L.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1157
Author(s):  
Francesco Nacca ◽  
Concetta Cozzolino ◽  
Petronia Carillo ◽  
Pasqualina Woodrow ◽  
Amodio Fuggi ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Performance ◽  
Internal Standard ◽  
Plant Tissues ◽  
Cellular Functions ◽  
Sulphur Compounds ◽  
Stress Protection ◽  
Automated Method ◽  
Reduced Forms

The high content of glucosinolates and glutathione makes the Brassicaceae an important healthy food. Thiols and especially glutathione and γ-Glu-Cys-Gly tripeptide are involved in many fundamental cellular functions such as oxidative stress protection. Although several methods for sulphur compounds analysis in biological samples are actually used, the determination of glutathione and other sulphur derivatives in plant tissues is rather problematic due to their extreme susceptibility to oxidation, which can lead to their overestimation. The aim of this work was the improvement and validation of an automated method for determination of reduced and oxidised glutathione, cysteine and γ-glutamylcysteine in plant tissues. The method consists of a fully automated pre-column derivatization of thiols based on monobromobimane reagent, a high-performance liquid chromatography derivatives separation, and a fluorimetric detection and quantification. The method was successfully applied for determination of the oxidized and reduced forms of Cys, γ-GC and GSH content in leaves, petioles, inflorescences and roots of Brassica rapa L. subsp. Sylvestris. At harvest, in freshly cut plants, the average contents of GSH/2GSSG were 840/45, 345/70 and 150/70 nmol g−1 FW for the florets, leaf blades and stems, respectively; those of Cys/2Cys were 80/12, 29/12 and 24/6 nmol g-1 FW; while those of γ-GC/γ-GCCG-γ were 8.0/4.0, and 6.0/3.0, 3.0/2.0 nmol g−1 FW, respectively. Such amounts were lower in low-sulphur-grown plants at harvest. The very low coefficient of variation between repeated tests (maximum 1.6%), the high recovery of internal standard (>96%) and the linear correlation coefficient of the calibration (R2 > 0.99) support the efficiency of this method that allowed analysing about 50 samples/die in a totally automated manner with no operator intervention. Our results show that the reported method integrations can significantly improve thiols detection via HPLC.

Aspects of the determination of free bile acids in gastric juice

1987 ◽  
Vol 327 (7) ◽  
pp. 729-730 ◽  
Author(s):  
T. O'Reilly ◽  
D. Thorburn Burns
Keyword(s):  
Bile Acids ◽  
Gastric Juice ◽  
Free Bile Acids

Automated Fluoride Ion Determination

1977 ◽  
Vol 14 (1-6) ◽  
pp. 269-274 ◽  
Author(s):  
David C. Cowell
Keyword(s):  
Continuous Flow ◽  
Ion Concentration ◽  
Fluoride Ion ◽  
Selective Electrode ◽  
Automated Method ◽  
Electrode Response ◽  
Kinetics Of ◽  
Fluoride Ion Selective Electrode ◽  
Flow Techniques

An automated method is described, using standard continuous flow techniques, for the determination of urine fluoride ion concentration using a fluoride ion selective electrode. It is shown that the kinetics of the electrode response to changes in fluoride ion can be used for the accurate measurement of fluoride ion concentration in urine, and that equilibration of the electrode response is not a prerequisite for the measurement of fluoride ion. Recovery experiments are in the range 83 to 90%; in-batch precision is between 0·9 and 1·6% and carryover 2·5% or less.

An automated method for the determination of N-acetylamino sugars

1965 ◽  
Vol 12 (3) ◽  
pp. 565-570 ◽  
Author(s):  
David A. Swann ◽  
Endre A. Balazs
Keyword(s):  
Automated Method
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