Lipid phase behaviour and lipid-protein interactions in the chloroplast photosynthetic membrane

1987 ◽  
Vol 15 (1) ◽  
pp. 86-91 ◽  
Author(s):  
PETER J. QUINN
Keyword(s):  
Protein Interactions ◽  
Phase Behaviour ◽  
Lipid Phase ◽  
Photosynthetic Membrane ◽  
Lipid Protein Interactions

Reconstitution of lipoproteins. II. Lipid–protein interaction between dimyristoyl-lecithin and dimyristoyl-lecithin:cholesterol vesicles and purified apolipoprotein C-I and C-III2 studied by isomeric spin-labelled lecithins

1980 ◽  
Vol 58 (7) ◽  
pp. 592-598 ◽  
Author(s):  
D. J. Vaughan ◽  
W. C. Breckenridge ◽  
N. Z. Stanacev
Keyword(s):  
Protein Interactions ◽  
Phase Changes ◽  
Phospholipid Bilayer ◽  
Head Group ◽  
Lipid Phase ◽  
Resonance Spectroscopy ◽  
Apolipoprotein C ◽  
Lipid Protein Interaction ◽  
Lipid Protein Interactions ◽  
Induced Changes

The reconstitution of purified apolipoprotein C-I and C-III2 with sn-3-dimyristoyl-lecithin and sn-3-dimyristoyl-lecithin:cholesterol (10:1) vesicles was studied by electron spin resonance spectroscopy using isomeric 5′-, 12′-, and 16′-(N-oxyl-4″,4″-dimethyloxazolidine)stearoyl spin-labelled lecithin probes. Results obtained from the temperature-induced changes of lipoprotein recombinants showed the hydrophilic nature of the lipid–protein interactions. The temperature-induced phospholipid phase transition, as measured by 5′-(N-oxyl-4″,4″-dimethyloxazolidine)stearoyl spin-labelled lecithin probe in recombinants containing apoprotein C-1 or apoprotein C-III2, is very broad and has a small cooperative unit indicative of extensive lipid–protein interactions occurring at the head group region of the phospholipid bilayer. When 12′- and 16′-(N-oxyl-4″,4″-dimethyloxazolidine)stearoyl spin-labelled lecithins are used as probes in the same system, similar sharper and more cooperative lipid phase changes are detected. These results indicate a surface location for both apoprotein C-I and apoprotein C-III2 with respect to the phospholipid bilayer in lipoprotein recombinants with and without cholesterol.

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