13C nuclear magnetic resonance studies in vivo on the metabolism of [1-13C]benzoate by mutants of Pseudomonas putida

1986 ◽  
Vol 14 (6) ◽  
pp. 1268-1269 ◽  
Author(s):  
ANTHONY E. G. CASS ◽  
DOUGLAS W. RIBBONS ◽  
JOHN T. ROSSITER ◽  
STEVE R. WILLIAMS
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Pseudomonas Putida ◽  
Magnetic Resonance Studies ◽  
13C Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

X-Ray crystallographic and 13C nuclear magnetic resonance studies of 3,4,5,6-tetrahydro-2H-1,3,4-oxadiazin-2-ones derived from ephedrine and pseudoephedrine

Tetrahedron ◽  
2001 ◽  
Vol 57 (49) ◽  
pp. 9789-9798 ◽  
Author(s):  
Shawn R Hitchcock ◽  
George P Nora ◽  
David M Casper ◽  
Michael D Squire ◽  
Christopher D Maroules ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Studies ◽  
X Ray ◽  
13C Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

scholarly journals Interactions between Pyruvate and Lactate Metabolism in Propionibacterium freudenreichii subsp.shermanii: In Vivo 13C Nuclear Magnetic Resonance Studies

2000 ◽  
Vol 66 (5) ◽  
pp. 2012-2020 ◽  
Author(s):  
Catherine Deborde ◽  
Patrick Boyaval
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Tricarboxylic Acid Cycle ◽  
Dry Weight ◽  
Resonance Spectroscopy ◽  
Propionibacterium Freudenreichii ◽  
Pyruvate Metabolism ◽  
13C Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

ABSTRACT In vivo 13C nuclear magnetic resonance spectroscopy was used to elucidate the pathways and the regulation of pyruvate metabolism and pyruvate-lactate cometabolism noninvasively in living-cell suspensions of Propionibacterium freudenreichiisubsp. shermanii. The most important result of this work concerns the modification of fluxes of pyruvate metabolism induced by the presence of lactate. Pyruvate was temporarily converted to lactate and alanine; the flux to acetate synthesis was maintained, but the flux to propionate synthesis was increased; and the reverse flux of the first part of the Wood-Werkman cycle, up to acetate synthesis, was decreased. Pyruvate was consumed at apparent initial rates of 148 and 90 μmol · min−1 · g−1 (cell dry weight) when it was the sole substrate or cometabolized with lactate, respectively. Lactate was consumed at an apparent initial rate of 157 μmol · min−1 · g−1when it was cometabolized with pyruvate. P. shermanii used several pathways, namely, the Wood-Werkman cycle, synthesis of acetate and CO2, succinate synthesis, gluconeogenesis, the tricarboxylic acid cycle, and alanine synthesis, to manage its pyruvate pool sharply. In both types of experiments, acetate synthesis and the Wood-Werkman cycle were the metabolic pathways used most.

Sign in / Sign up

Export Citation Format

Share Document