Mitogenic activity of foetal calf serum is completely removed by affinity chromatography using Blue Sepharose

1986 ◽  
Vol 14 (6) ◽  
pp. 1088-1088
Author(s):  
PAMELA O. LAMINA ◽  
PETER KNOX
Keyword(s):  
Affinity Chromatography ◽  
Calf Serum ◽  
Mitogenic Activity ◽  
Foetal Calf Serum ◽  
Blue Sepharose

Potassium and calcium currents activated by foetal calf serum in Balb-c 3T3 fibroblasts

1992 ◽  
Vol 1112 (2) ◽  
pp. 241-245 ◽  
Author(s):  
Davide Lovisolo ◽  
Luca Munaron ◽  
Francesco M. Baccino ◽  
Gabriella Bonelli
Keyword(s):  
Calf Serum ◽  
Calcium Currents ◽  
Foetal Calf Serum ◽  
3T3 Fibroblasts

scholarly journals Rotavirus Persistence in Cell Cultures: Selection of Resistant Cells in the Presence of Foetal Calf Serum

1983 ◽  
Vol 64 (5) ◽  
pp. 1101-1110 ◽  
Author(s):  
A. Chiarini ◽  
S. Arista ◽  
A. Giammanco ◽  
A. Sinatra
Keyword(s):  
Cell Cultures ◽  
Calf Serum ◽  
Foetal Calf Serum ◽  
Selection Of ◽  
Resistant Cells

Restriction of foetal calf serum-induced cytotoxicity by human T-cells

1981 ◽  
Vol 3 (2) ◽  
pp. 81-85 ◽  
Author(s):  
I.S. Misko ◽  
R.G. Kane ◽  
J.H. Pope
Keyword(s):  
T Cells ◽  
Calf Serum ◽  
Foetal Calf Serum ◽  
Human T Cells

Drug activity against Onchocerca gutturosa males in vitro: a model for chemotherapeutic research on onchocerciasis

1987 ◽  
Vol 61 (4) ◽  
pp. 271-281 ◽  
Author(s):  
Simon Townson ◽  
C. Connelly ◽  
A. Dobinson ◽  
R. Muller
Keyword(s):  
Culture System ◽  
Serum Proteins ◽  
Good Condition ◽  
Calf Serum ◽  
New Drugs ◽  
Foetal Calf Serum ◽  
Partial Recovery ◽  
Feeder Cells ◽  
Compound Identification

ABSTRACTAn in vitro system for chemotherapeutic research using adult male Onchocerca gutturosa has been developed as a model for O. volvulus. Using a culture system consisting of medium MEM+10% heat inactivated foetal calf serum (IFCS)+LLCMK2 (monkey kidney) feeder cells in an atmosphere of 5% CO2 in air, we examined the effects of a range of antiparasitic drugs on worm motility. Ivermectin, levamisole, furapyrimidone, Mel W, chloroquine, metrifonate, flubendazole, amoscanate and the Ciba-Geigy compounds CGP 6140, CGP 20′376 and CGI 17658 either immobilized or significantly reduced motility levels at a concentration of 5x10−5M or less within a 7-day period. Worms were affected at very low concentrations by ivermectin (effective conc. to reduce motility levels to 50% of controls, 3.14x10−8M), levamisole (7.95x10−8M), CGP 6140 (8.87x10−9M) and CGP 20′376 (2.78x10−8M). Difficulties were experienced in accurately repeating the immotile endpoint for levamisole due to an inconsistent partial recovery of motility. Over a 7-day period diethylcarbamazine had little effect on motility levels, while suramin caused a slight increase in activity compared to controls at some timepoints. Subsequent experiments demonstrated some differences in drug efficacy depending on the presence or absence of serum and feeder cells in the culture system probably because of drug avidly binding to serum proteins. However, serum and cells were found to be essential ingredients of the culture system to maintain worms in good condition, indicating that new drugs should be evaluated both in the presence and absence of serum and cells. Comparisons were made between the responses of O. gutturosa and Brugia pahangi to certain drugs and these species were found to significantly differ in their sensitivities to ivermectin and a novel compound (Wellcome), indicating that Onchocerca parasites should be used wherever possible for compound identification and development intended for the treatment of onchocerciasis. The in vitro system described here, using male O. gutturosa, provides a basis for further research and a practical alternative to O. volvulus.

‘Spontaneous’ sex reversal in organ cultures of the embryonic male gonad of the bird

Development ◽  
1974 ◽  
Vol 31 (3) ◽  
pp. 611-620
Author(s):  
Gregory F. Erickson
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Calf Serum ◽  
Sex Reversal ◽  
Biologically Active ◽  
Foetal Calf Serum ◽  
Developmental Sequence ◽  
Organ Cultures ◽  
Male Gonad ◽  
Ovarian Cortex

The left embryonic testis of the bird (4–8 days of incubation) was organ cultured in medium that contained 10% foetal calf serum. Under these conditions, the germinal epithelium (GE) of the 4-day gonad differentiates into an ovarian cortex and the male primordial germ cells (PGCs) complete a developmental sequence similar to normal oocytes, i.e. they divide mitotically, develop a Balbiani body, divide synchronously in groups of two, four, and eight germ cells, and some enter pre-leptotene. No medullary tissue develops in the 4-day explants. The pieces of 6- and 8-day gonad differentiate into true ovotestes in which the GE develops into a cortex and the medulla develops into seminiferous cords. The PGCs in the cortex differentiate as oocytes and those in the seminiferous cords differentiate as spermatogonia. The possibility that biologically active oestrogens are present in the growth medium is discussed.

Substitution of transferrin by FeCl3 in the development of a low foetal calf serum concentration medium for KB-26.5 hybridoma cell line

1993 ◽  
Vol 13 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Bo Damgaard ◽  
Anna Sanfeliu ◽  
Jordi Joan Cair� ◽  
Carles Casas ◽  
Carles Sol� ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Cell Line ◽  
Calf Serum ◽  
Foetal Calf Serum

scholarly journals Molecular analysis of bovine viral diarrhoea virus isolates from South Africa

2003 ◽  
Vol 70 (4) ◽  
Author(s):  
N. Kabongo ◽  
C. Baule ◽  
M. Van Vuuren
Keyword(s):  
South Africa ◽  
Southern Africa ◽  
Calf Serum ◽  
Bovine Viral Diarrhoea Virus ◽  
Border Disease Virus ◽  
Foetal Calf Serum ◽  
Type I ◽  
Bovine Viral Diarrhoea ◽  
Border Disease ◽  
Diarrhoea Virus

The presence of bovine viral diarrhoea virus in South Africa has been confirmed by several serological surveys. However, little is known about its biological properties. Twenty five isolates obtained by isolation in tissue culture and detected by means of the antigen capture ELISA from clinically sick cattle and from foetal calf serum in South Africa were characterized on the basis of analysis of the 5' non-translated (NTR) region of the genome. A reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify specific sequences from the 5'NTR of the genome. The oligonucleotide primers corresponding to positions 105-125 and 399-378, respectively, in the sequence of BVDV strain NADL were used to generate the PCR products. Both strands were sequenced directly with these primers and fluorescence-labelled dideoxynucleotides in an automated nucleic acid sequencer. Reference strains of pestiviruses [(BVDV type I, BVDV type II, border disease virus (BDV) and hog cholera virus (HCV)] and isolates from a previous investigation on BVDV in southern Africa were included for comparative purposes. All the BVDV strains obtained during this study belong to subgroups of BVDV genotype I. No association could be demonstrated between the geographic origin of the isolates. A number of isolates formed another branch separate from the existing branches Ia, Ib and Ic. These findings suggest that extensive genetic diversity can be found within BVDV type I isolates from southern Africa. Isolates that group with the classical BVDV type I strains, particularly of American origin, coexist with variants that appear to represent a local genetic pool and or variants evolving from the classical strains.

Maturation of porcine oocytes for 33 or 44 hours in vitro in the presence or absence of serum

1998 ◽  
Vol 1998 ◽  
pp. 180-180
Author(s):  
K. Rust ◽  
M.E. Staines ◽  
GJ. McCallum ◽  
N.S. Prathalingam ◽  
S.A. Edwards ◽  
...  
Keyword(s):  
Disease Transmission ◽  
Calf Serum ◽  
International Markets ◽  
Foetal Calf Serum ◽  
Maturation Time ◽  
Nuclear Maturation ◽  
Defined Media ◽  
Porcine Embryo ◽  
Disease Risks

Porcine embryo production in vitrois providing the impetus for the development of cryopreservation strategies aimed at welfare-friendly domestic and international marketing and movement of stock in a manner that minimises risks of disease transmission. In the context of disease risks, defined media, which avoid the use of serum and other biohazardous products, are likely to become essential in the production of embryos for international markets. In preparing for this situation, the present comparative study investigated in vitronuclear maturation of porcine oocytes in the presence of either foetal calf serum (FCS) or polyvinyl alcohol (PVA). In addition, the effect of restricting the maturation time to 33 rather than 44 hours was examined.

Sign in / Sign up

Export Citation Format

Share Document