Transfer RNA genes in Pseudomonas aeruginosa

1986 ◽  
Vol 14 (4) ◽  
pp. 725-726
Author(s):  
MARGARET A. HUGHES ◽  
DAVID S. JONES
1999 ◽  
Vol 45 (9) ◽  
pp. 791-796 ◽  
Author(s):  
Andrew M Kropinski ◽  
Mary Jo Sibbald

Using tRNAscan-SE and FAStRNA we have identified four tRNA genes in the delayed early region of the bacteriophage D3 genome (GenBank accession No. AF077308). These are specific for methionine (AUG), glycine (GGA), asparagine (AAC), and threonine (ACA). The D3 Thr- and Gly-tRNAs recognize codons, which are rarely used in Pseudomonas aeruginosa and presumably, influence the rate of translation of phage proteins. BLASTN searches revealed that the D3 tRNA genes have homology to tRNA genes from Gram-positive bacteria. Analysis of codon usage in the 91 ORFs discovered in D3 indicates patterns of codon usage reminiscent of Escherichia coli or P. aeruginosa.Key words: bacteriophage, Pseudomonas, D3, tRNA, codon usage.


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
J. Antonio Baeza ◽  
Fabiola A. Sepúlveda ◽  
M. Teresa González

Abstract Background The monogenean Benedenia seriolae parasitizes fishes belonging to the genus Seriola, represents a species complex, and causes substantial impact on fish welfare in aquaculture systems worldwide. This study reports, for the first time, the complete mitochondrial genome of B. humboldti n. sp., a new cryptic species from the South-East Pacific (SEP). Methods The mitogenome of B. humboldti n. sp. was assembled from short Illumina 150 bp pair-end reads. The phylogenetic position of B. humboldti n. sp. among other closely related congeneric and confamiliar capsalids was examined using mitochondrial protein-coding genes (PCGs). Morphology of B. humboldti n. sp. was examined based on fixed and stained specimens. Results The AT-rich mitochondrial genome of B. humboldti is 13,455 bp in length and comprises 12 PCGs (atp8 was absent as in other monogenean genomes), 2 ribosomal RNA genes, and 22 transfer RNA genes. All protein-coding, ribosomal RNA, and transfer RNA genes are encoded on the H-strand. The gene order observed in the mitochondrial genome of B. humboldti n. sp. was identical to that of B. seriolae from Japan but different from that of B. seriolae from Australia. The genetic distance between B. humboldti n. sp. and B. seriolae from Japan was high. Minor but reliable differences in the shape of the penis were observed between Benedenia humboldti n. sp. and congeneric species. Conclusions Phylogenetic analyses based on PCGs in association with differences in the shape of the penis permitted us to conclude that the material from the South-East Pacific represents a new species of Benedenia infecting S. lalandi off the coast of Chile. The discovery of this parasite represents the first step to improving our understanding of infestation dynamics and to develop control strategies for this pathogen infecting the farmed yellowtail kingfish, Seriola lalandi, in the South-East Pacific.


1986 ◽  
Vol 188 (3) ◽  
pp. 343-353 ◽  
Author(s):  
Wolf-Dietrich Heyer ◽  
Peter Münz ◽  
Hanspeter Amstutz ◽  
Riccardo Aebi ◽  
Cristoph Gysler ◽  
...  

1979 ◽  
Vol 170 (3) ◽  
pp. 261-275 ◽  
Author(s):  
Micheline Wesolowski ◽  
Hiroshi Fukuhara

1976 ◽  
Vol 3 (9) ◽  
pp. 2379-2386 ◽  
Author(s):  
H. Feldman
Keyword(s):  

EMBO Reports ◽  
2008 ◽  
Vol 9 (9) ◽  
pp. 820-820 ◽  
Author(s):  
Massimo Di Giulio
Keyword(s):  

1983 ◽  
Vol 93 (4) ◽  
pp. 1101-1108 ◽  
Author(s):  
Atsushi MIYAJTMA ◽  
Takashi YOKOTA ◽  
Yutaka TAKEBE ◽  
Moriluiko NAKAMURA ◽  
Yoshito KAZIRO

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