Influence of vitamin D metabolites on proteoglycan synthesis by chick growth cartilage in organ culture

1986 ◽  
Vol 14 (1) ◽  
pp. 113-114 ◽  
Author(s):  
I. R. DICKSON
Keyword(s):  
Vitamin D ◽  
Organ Culture ◽  
Proteoglycan Synthesis ◽  
Vitamin D Metabolites ◽  
Chick Growth ◽  
Growth Cartilage

The influence of vitamin D metabolites on collagen synthesis by chick cartilage in organ culture

1985 ◽  
Vol 105 (1) ◽  
pp. 79-85 ◽  
Author(s):  
I. R. Dickson ◽  
P. M. Maher
Keyword(s):  
Vitamin D ◽  
Collagen Synthesis ◽  
Bone Cells ◽  
Endochondral Bone Formation ◽  
Primary Role ◽  
Vitamin D Metabolites ◽  
Minimum Concentration ◽  
Dihydroxyvitamin D ◽  
Growth Cartilage ◽  
1,25 Dihydroxyvitamin D

ABSTRACT When growth cartilage from rachitic chicks was cultured in the presence of the calcium-regulating hormone 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), collagen resorption was increased and collagen synthesis decreased compared to control cultures containing no hormone. The minimum concentration of the hormone that caused a statistically significant inhibition of collagen synthesis was 10 −8 mol/l. Collagen synthesis by growth cartilage from normal chicks was also reduced by 1,25-(OH)2D3, showing that it was not an abnormal response of vitamin D-depleted tissue. 25-Hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3 also inhibited collagen synthesis by cultures of growth cartilage but only at higher metabolite concentrations. 1,25-Dihydroxyvitamin D3 (10−7 mol/l) did not significantly inhibit collagen synthesis by cultures of articular fibrocartilage and of sternal cartilage, tissues that do not calcify physiologically. The minimum concentration of 1,25-(OH)2D3 (10−9 mol/l) necessary to cause decreased collagen synthesis by embryonic chick calvaria was lower than the value obtained with growth cartilage; this suggests that bone cells may be more sensitive to the hormone in this respect than are growth cartilage chondrocytes. These findings provide evidence of a direct role of 1,25-(OH)2D3 in the control of endochondral bone formation which is consistent with its primary role in the maintenance of plasma calcium homeostasis. J. Endocr. (1985) 105, 79–85

Comparison of the effects of vitamin D metabolites on collagen synthesis and resportion of fetal rat bone in organ culture

1980 ◽  
Vol 32 (1) ◽  
pp. 135-138 ◽  
Author(s):  
Lawrence G. Raisz ◽  
Barbara E. Kream ◽  
Mark D. Smith ◽  
Hollis A. Simmons
Keyword(s):  
Vitamin D ◽  
Organ Culture ◽  
Collagen Synthesis ◽  
Vitamin D Metabolites ◽  
Fetal Rat ◽  
Rat Bone

Pathology of Experimental Vitamin D Deficiency in Chickens and Effects of Treatment with Vitamin D Metabolites

1981 ◽  
Vol 18 (5) ◽  
pp. 638-651 ◽  
Author(s):  
N. F. Cheville ◽  
R. L. Horst
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Structural Changes ◽  
Bone Lesions ◽  
Vitamin D Metabolites ◽  
Deficient Diet ◽  
Ultimobranchial Body ◽  
Growth Cartilage ◽  
Bone Deformities

Structural changes in bone, parathyroid, and ultimobranchial body were examined in three groups of chicks fed a vitamin D-deficient diet; one group was treated with vitamin D3 and another with 1,25(OH)2D3. Diets were fed from day of hatching until 5 weeks old, when deficient chicks were near death due to hypocalcemic tetany, loss of fat and muscle, and marked bone deformities. In deficient chicks, parathyroid mass increased linearly to 7.5 times normal at 5 weeks. Parathyroid cells were irregular and vacuolated, with few granules. Vitamin D3 treatment (daily from hatching) prevented these changes. Chicks treated with 1,25(OH)2D3 had normal parathyroids until the fifth week, when parathyroid mass increased greatly. There were few differences in length of growth cartilage, but marked changes in length of metaphyses. Deficient chicks had metaphyses nearly five times longer than vitamin D3-treated chicks. Metaphyses in chicks given 1,25(OH)2D3 were twice as long as those of vitamin D-treated chicks at 5 weeks. Both osteoblasts and osteoclasts were more numerous in deficient chicks. These studies suggest that vitamin D3 is more effective than 1,25(OH)2D3 in preventing parathyroid and bone lesions of vitamin D deficiency.

2223-PUB: Association of Vitamin D Metabolites with Type 2 Diabetes Complications in Qatar

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2223-PUB
Author(s):  
STEPHEN ATKIN ◽  
ALEXANDRA E. BUTLER
Keyword(s):  
Type 2 Diabetes ◽  
Vitamin D ◽  
Diabetes Complications ◽  
Vitamin D Metabolites

Vitamin D metabolites in diabetic patients: Decreased serum concentration of 24,25-dihydroxyvitamin D

1982 ◽  
Vol 42 (6) ◽  
pp. 487-491 ◽  
Author(s):  
Claus Christiansen ◽  
Merete Sanvig Christensen ◽  
Peter McNair ◽  
Britta Nielsen ◽  
Steen Madsbad
Keyword(s):  
Vitamin D ◽  
Serum Concentration ◽  
Diabetic Patients ◽  
Vitamin D Metabolites ◽  
Dihydroxyvitamin D
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