A study of [U-14C]ketoisoleucine degradation by isolated rat liver mitochondria using reverse-phase high-performance liquid chromatography of intact acyl coenzyme A esters with on-line radiochemical detection

1985 ◽  
Vol 13 (6) ◽  
pp. 1220-1221 ◽  
Author(s):  
ANTHONY G. CAUSEY ◽  
LORANNE AGIUS ◽  
KIM BARTLETT
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Reverse Phase ◽  
Isolated Rat Liver ◽  
On Line ◽  
Acyl Coenzyme A ◽  
Coenzyme A Esters ◽  
Isolated Rat

scholarly journals A study of the metabolism of [U-14C]3-methyl-2-oxopentanoate by rat liver mitochondria using h.p.l.c. with continuous on-line monitoring of radioactive intact acyl-coenzyme A intermediates

1986 ◽  
Vol 235 (2) ◽  
pp. 343-350 ◽  
Author(s):  
A G Causey ◽  
B Middleton ◽  
K Bartlett
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Intermediary Metabolism ◽  
Rat Liver Mitochondria ◽  
Powerful Method ◽  
Reverse Phase ◽  
Mitochondrial Fractions ◽  
On Line ◽  
Riboflavin Deficient ◽  
Acyl Coenzyme A

A reverse-phase h.p.l.c. system for the resolution of the acyl-CoA intermediates of the degradation of 3-methyl-2-oxopentanoate is described. The validation of a method for the measurement of radioactive intermediates produced by the incubation of [U-14C]3-methyl-2-oxopentanoate with rat liver mitochondrial fractions is described. The absence of bicarbonate caused the accumulation of [14C]propionyl-CoA. The accumulation of [14C]2-methylbutyryl-CoA was observed in incubations with mitochondrial fractions derived from riboflavin-deficient animals. Studies of the accumulation of labelled intermediates with time suggest that there is regulation of the pathway of isoleucine degradation at methylmalonyl-CoA mutase, as suggested for valine [Corkey, Martin-Requero, Walajtys-Rode, Williams & Williamson (1982) J. Biol. Chem. 257, 9668-9676]. These studies demonstrate that h.p.l.c. with on-line continuous radiochemical detection is a powerful method for the investigation of the control of intermediary metabolism.

Interaction of aromatic aldehydes with isolated rat liver mitochondria

1982 ◽  
Vol 31 (11) ◽  
pp. 2025-2029 ◽  
Author(s):  
Charles R. Wolf ◽  
Helen Harmon ◽  
Carol M. Schiller
Keyword(s):  
Rat Liver ◽  
Aromatic Aldehydes ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Isolated Rat Liver ◽  
Isolated Rat
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